Anti-Choline Acetyltransferase antibody (ab18736)


  • Product nameAnti-Choline Acetyltransferase antibody
    See all Choline Acetyltransferase primary antibodies
  • Description
    Sheep polyclonal to Choline Acetyltransferase
  • Tested applicationsIHC-FoFr, IHC-P, WB, ICC/IFmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Rabbit, Guinea pig, Human, Pig
    Predicted to work with: Chicken
  • Immunogen

    Synthetic peptide:


    conjugated to KLH, corresponding to amino acids 167-188 of Pig Choline Acetyltransferase.



Our Abpromise guarantee covers the use of ab18736 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-FoFr 1/1000.

Use on perfusion fixed tissue (1% sodium Nitrite in 0.1M TBS, pH, 7.4 for 20 min then 4% formaldehyde containing 15% picric acid in 0.1M TBS,pH, 7.4 for 20min). Please note sections must be post fixed if the tissue is cut freshly.

IHC-P Use at an assay dependent concentration.
WB Use at an assay dependent concentration. Predicted molecular weight: 83 kDa.
ICC/IF 1/100. PubMed: 18393628


  • FunctionCatalyzes the reversible synthesis of acetylcholine (ACh) from acetyl CoA and choline at cholinergic synapses.
  • Involvement in diseaseDefects in CHAT are the cause of congenital myasthenic syndrome with episodic apnea (CMSEA) [MIM:254210]; formerly known as familial infantile myasthenia gravis 2 (FIMG2). CMSEA is an autosomal recessive congenital myasthenic syndrome. Patients have myasthenic symptoms since birth or early infancy, negative tests for anti-AChR antibodies, and abrupt episodic crises with increased weakness, bulbar paralysis, and apnea precipitated by undue exertion, fever, or excitement.
  • Sequence similaritiesBelongs to the carnitine/choline acetyltransferase family.
  • Information by UniProt
  • Database links
  • Alternative names
    • Acetyl CoA choline O acetyltransferase antibody
    • Acetyl CoA:choline O acetyltransferase antibody
    • ChAT antibody
    • CHOACTase antibody
    • Choline acetylase antibody
    • choline acetyltransferase antibody
    • Choline O acetyltransferase antibody
    • Choline O-acetyltransferase antibody
    • CLAT_HUMAN antibody
    • CMS1A antibody
    • CMS1A2 antibody
    • EC antibody
    • OTTHUMP00000019583 antibody
    • OTTHUMP00000019584 antibody
    see all

Anti-Choline Acetyltransferase antibody images

  • ab18736 staining Choline Acetyltransferase in mouse brain tissue sections by Immunohistochemistry (PFA perfusion fixed frozen sections). Tissue samples were fixed by perfusion with paraformaldehyde, permeablized with 0.3% Triton X-100 and blocked with 3% donkey serum in 0.3% Triton X-100/TBS for 1 hour at 21°C. The sample was incubated with primary antibody (1/1000 in 1% donkey serum + 0.3% Triton X-100/TBS) at 4°C for 24 hours. An Alexa Fluor® 594-conjugated donkey anti-sheep polyclonal (1/200) was used as the secondary antibody.

    See Abreview

  • Immunohistochemical detection of ChAT in rat basal forebrain using Sheep anti ChAT (ab18736).

    Rat was perfused with 1% sodium Nitrite in 0.1M TBS, pH, 7.4 for 20 min. and then fixed with 4% formaldehyde containing 15% picric acid in 0.1M TBS, pH, 7.4 for 30 min. The brain was then cryoprotected in 30% overnight and cut into 40 nm free-floating sections. Sections were washed 2x with TBS for 20 min. and passed through asending ethanol and finally transferred to 0.1 M TBS for rehydration for 1h.

References for Anti-Choline Acetyltransferase antibody (ab18736)

This product has been referenced in:
  • Yu B  et al. Mechanisms Underlying Footshock and Psychological Stress-Induced Abrupt Awakening From Posttraumatic "Nightmares". Int J Neuropsychopharmacol N/A:N/A (2015). Rat . Read more (PubMed: 26591007) »
  • Hadzipasic M  et al. Selective degeneration of a physiological subtype of spinal motor neuron in mice with SOD1-linked ALS. Proc Natl Acad Sci U S A 111:16883-8 (2014). IHC ; Mouse . Read more (PubMed: 25385594) »

See all 7 Publications for this product

Product Wall

Application Immunohistochemistry (PFA perfusion fixed frozen sections)
Blocking step Donkey Serum in 0.3% Triton X-100/TBS as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 21°C
Antigen retrieval step None
Sample Mouse Tissue sections (Brain)
Specification Brain
Permeabilization Yes - 0.3% Triton X-100
Fixative Paraformaldehyde

Ms. Carla Cabral

Verified customer

Submitted Mar 28 2014

Thank you very much for your interest in our products. The following antibodies are tested and guaranteed for IHC-P in rat. Unfortunately, we do not have blocking peptide for these antibodies in our catalogue. ab68779 Click here (or use...

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Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample Rat Tissue sections (brain CA1)
Specification brain CA1
Fixative Paraformaldehyde
Antigen retrieval step Heat mediated - Buffer/Enzyme Used: EDTA
Permeabilization Yes - 0.1% IGEPAL CA630
Blocking step BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 37°C

Abcam user community

Verified customer

Submitted Apr 10 2009

Thank you for your enquiry. Please find below the answers to your questions: Q1. was the fix of 4% formaldehyde containing 15% picric acid in 0.1M TBS,pH, 7.4 for 30 min done after the brain was extracted, and if so was the whole brain simply immer...

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