Anti-Choline Acetyltransferase antibody (ab18736)

Overview

Properties

Applications

Our Abpromise guarantee covers the use of ab18736 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-FoFr
IHC-P
WB
ICC/IF
  • Application notesICC/IF: 1/100 (PMID 18393628).
    IHC-P: Use at an assay dependent dilution; (see recommended protocol). Use on perfusion fixed tissue (1% sodium Nitrite in 0.1M TBS, pH, 7.4 for 20 min then 4% formaldehyde containing 15% picric acid in 0.1M TBS,pH, 7.4 for 20min).
    IHC-FoFr: Use at a concentration of 1 µg/ml (see recommended protocol). Use on perfusion fixed tissue (1% sodium Nitrite in 0.1M TBS, pH, 7.4 for 20 min then 4% formaldehyde containing 15% picric acid in 0.1M TBS,pH, 7.4 for 20min). Please note sections must be post fixed if the tissue is cut freshly.
    WB: Use at an assay dependent dilution. Predicted molecular weight: 83 kDa.


    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • Target

    • FunctionCatalyzes the reversible synthesis of acetylcholine (ACh) from acetyl CoA and choline at cholinergic synapses.
    • Involvement in diseaseDefects in CHAT are the cause of congenital myasthenic syndrome with episodic apnea (CMSEA) [MIM:254210]; formerly known as familial infantile myasthenia gravis 2 (FIMG2). CMSEA is an autosomal recessive congenital myasthenic syndrome. Patients have myasthenic symptoms since birth or early infancy, negative tests for anti-AChR antibodies, and abrupt episodic crises with increased weakness, bulbar paralysis, and apnea precipitated by undue exertion, fever, or excitement.
    • Sequence similaritiesBelongs to the carnitine/choline acetyltransferase family.
    • Information by UniProt
    • Database links
    • Alternative names
      • Acetyl CoA choline O acetyltransferase antibody
      • acetyl CoA:choline O acetyltransferase antibody
      • ChAT antibody
      • CHOACTase antibody
      • Choline acetylase antibody
      • choline acetyltransferase antibody
      • Choline O acetyltransferase antibody
      • Choline O acetyltransferase antibody
      • Choline O-acetyltransferase antibody
      • CLAT_HUMAN antibody
      • CMS1A antibody
      • CMS1A2 antibody
      • EC 2.3.1.6 antibody
      • OTTHUMP00000019583 antibody
      • OTTHUMP00000019584 antibody
      see all

    Anti-Choline Acetyltransferase antibody images

    • ab18736 staining Choline Acetyltransferase in mouse brain tissue sections by Immunohistochemistry (PFA perfusion fixed frozen sections). Tissue samples were fixed by perfusion with paraformaldehyde, permeablized with 0.3% Triton X-100 and blocked with 3% donkey serum in 0.3% Triton X-100/TBS for 1 hour at 21°C. The sample was incubated with primary antibody (1/1000 in 1% donkey serum + 0.3% Triton X-100/TBS) at 4°C for 24 hours. An Alexa Fluor® 594-conjugated donkey anti-sheep polyclonal (1/200) was used as the secondary antibody.

      See Abreview

    • Immunohistochemical detection of ChAT in rat basal forebrain using Sheep anti ChAT (ab18736).

      Rat was perfused with 1% sodium Nitrite in 0.1M TBS, pH, 7.4 for 20 min. and then fixed with 4% formaldehyde containing 15% picric acid in 0.1M TBS, pH, 7.4 for 30 min. The brain was then cryoprotected in 30% overnight and cut into 40 nm free-floating sections. Sections were washed 2x with TBS for 20 min. and passed through asending ethanol and finally transferred to 0.1 M TBS for rehydration for 1h.

    References for Anti-Choline Acetyltransferase antibody (ab18736)

    This product has been referenced in:
    • Sims RE  et al. Sleep-wake sensitive mechanisms of adenosine release in the basal forebrain of rodents: an in vitro study. PLoS One 8:e53814 (2013). ICC/IF ; Rat . Read more (PubMed: 23326515) »
    • Baracchi F  et al. Sepsis-induced alterations in sleep of rats. Am J Physiol Regul Integr Comp Physiol 301:R1467-78 (2011). IHC-FoFr ; Rat . Read more (PubMed: 21900639) »

    See all 5 Publications for this product

    Product Wall

    Application Immunohistochemistry (PFA perfusion fixed frozen sections)
    Blocking step Donkey Serum in 0.3% Triton X-100/TBS as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 21°C
    Antigen retrieval step None
    Sample Mouse Tissue sections (Brain)
    Specification Brain
    Permeabilization Yes - 0.3% Triton X-100
    Fixative Paraformaldehyde
    Username

    Ms. Carla Cabral

    Verified customer

    Submitted Mar 28 2014

    Thank you very much for your interest in our products. The following antibodies are tested and guaranteed for IHC-P in rat. Unfortunately, we do not have blocking peptide for these antibodies in our catalogue. ab68779 Click here (or use...

    Read More
    Abcam guarantees this product to work in the species/application used in this Abreview.
    Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Sample Rat Tissue sections (brain CA1)
    Specification brain CA1
    Fixative Paraformaldehyde
    Antigen retrieval step Heat mediated - Buffer/Enzyme Used: EDTA
    Permeabilization Yes - 0.1% IGEPAL CA630
    Blocking step BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 37°C
    Username

    Abcam user community

    Verified customer

    Submitted Apr 10 2009

    Thank you for your enquiry. Please find below the answers to your questions: Q1. was the fix of 4% formaldehyde containing 15% picric acid in 0.1M TBS,pH, 7.4 for 30 min done after the brain was extracted, and if so was the whole brain simply immer...

    Read More

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"