For the best experience on the Abcam website please upgrade to a modern browser such as Google Chrome.
Synthetic peptide: GLFSSYRLPGHTQDTLVAQKSS conjugated to KLH, corresponding to amino acids 167-188 of Pig Choline Acetyltransferase.
Our Abpromise guarantee covers the use of ab18736 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Immunohistochemical detection of ChAT in rat basal forebrain using Sheep anti ChAT (ab18736).
Rat was perfused with 1% sodium Nitrite in 0.1M TBS, pH, 7.4 for 20 min. and then fixed with 4% formaldehyde containing 15% picric acid in 0.1M TBS, pH, 7.4 for 30 min. The brain was then cryoprotected in 30% overnight and cut into 40 nm free-floating sections. Sections were washed 2x with TBS for 20 min. and passed through asending ethanol and finally transferred to 0.1 M TBS for rehydration for 1h.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"