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- Proteins and Peptides
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Product name
Anti-Choline Acetyltransferase antibody
See all Choline Acetyltransferase products (16) ...
Description
Goat polyclonal to Choline Acetyltransferase
Specificity
ab27444 is expected to recognise both reported isoforms.
Tested applications
ELISA, WBmore details
Cross reactivity
Reacts with
Human
Predicted to work with
Dog, Pig, Chimpanzee
Immunogen
Positive control
Human Placenta.
Properties
Form
Liquid
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage buffer
Preservative: 0.02% Sodium Azide
Constituents: 0.5% BSA, 5mg/ml Tris, pH 7.3
Concentration
Concentration information loading...
Purity
Immunogen affinity purified
Purification notes
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Clonality
Polyclonal
Isotype
IgG
Research areas
Neuroscience >> Cell Type Marker >> Neuron marker >> Soma marker
Neuroscience >> Neurotransmitter >> Acetylcholine
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Western blot - Choline Acetyltransferase antibody (ab27484)
(enlarge)
Applications
Show applications key
Our Abpromise guarantee covers the use of ab27484 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
ELISA
ELISA: 1/32000.ELISA: 1/32000.
WB: Use a concentration of 0.03 - 0.3 µg/ml. Detects a band of approximately 70 kDa (predicted molecular weight: 83 kDa).Can be blocked with Choline Acetyltransferase peptide (736-748) (ab45678).
Target
Function
Catalyzes the reversible synthesis of acetylcholine (ACh) from acetyl CoA and choline at cholinergic synapses.
Involvement in disease
Defects in CHAT are the cause of congenital myasthenic syndrome with episodic apnea (CMSEA) [MIM:254210]; formerly known as familial infantile myasthenia gravis 2 (FIMG2). CMSEA is an autosomal recessive congenital myasthenic syndrome. Patients have myasthenic symptoms since birth or early infancy, negative tests for anti-AChR antibodies, and abrupt episodic crises with increased weakness, bulbar paralysis, and apnea precipitated by undue exertion, fever, or excitement.
Sequence similarities
Belongs to the carnitine/choline acetyltransferase family.
Target information above from: UniProt accessionP28329
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Alternative names
- Choline O acetyltransferase antibody
- Acetyl CoA choline O acetyltransferase antibody
- acetyl CoA:choline O acetyltransferase antibody
see all
Database links
- Entrez Gene: 1103 Human
- Omim: 118490 Human
- SwissProt: P28329 Human
- Unigene: 302002 Human
Anti-Choline Acetyltransferase antibody images:
Western blot - Choline Acetyltransferase antibody (ab27484)
Anti-Choline Acetyltransferase antibody (ab27484) at 0.03 µg/ml + Human placenta lysate at 35 µg
Predicted band size : 83 kDa
Observed band size : 70 kDa (why is the actual band size different from the predicted?)
Primary incubation was 1 hour. Detected by chemiluminescence.
References for Anti-Choline Acetyltransferase antibody (ab27484)
ab27484 has not yet been referenced specifically in any publications.
Publishing research using ab27484? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"