Anti-Choline Acetyltransferase antibody (ab68779)

Overview

• Product nameAnti-Choline Acetyltransferase antibodySee all Choline Acetyltransferase primary antibodies ...
• Description
  Rabbit polyclonal to Choline Acetyltransferase
• Tested applicationsWB, IHC-P, IHC-Fr, ICC/IF more details
• Species reactivity
  Reacts with: Mouse, Rat, Guinea pig, Dog, Human, Pig
  
• Immunogen

  A synthetic peptide as a part of human, rat and mouse Choline Acetyltransferase conjugated to an immunogenic carrier protein.

Properties

• FormLiquid
• Storage instructionsStore at +4°C short term (1-2 weeks). Add glycerol to a final volume of 50%, aliquot and store at -20°C. Avoid repeated freeze / thaw cycles.
• Storage bufferPreservative: None
  Constituents: PBS
• Concentration information loading...
• PurityIgG fraction
• Clonality Polyclonal
• IsotypeIgG
• Research Areas
  • Neuroscience
  • Cell Type Marker
  • Neuron marker
  • Soma marker

  • Neuroscience
  • Neurotransmitter
  • Acetylcholine

Applications

Target

• FunctionCatalyzes the reversible synthesis of acetylcholine (ACh) from acetyl CoA and choline at cholinergic synapses.
• Involvement in diseaseDefects in CHAT are the cause of congenital myasthenic syndrome with episodic apnea (CMSEA) [MIM:254210]; formerly known as familial infantile myasthenia gravis 2 (FIMG2). CMSEA is an autosomal recessive congenital myasthenic syndrome. Patients have myasthenic symptoms since birth or early infancy, negative tests for anti-AChR antibodies, and abrupt episodic crises with increased weakness, bulbar paralysis, and apnea precipitated by undue exertion, fever, or excitement.
• Sequence similaritiesBelongs to the carnitine/choline acetyltransferase family.

Target information above from: UniProt accession P28329 The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010) .

Information by UniProt
• Database links
  • Entrez Gene: 1103 Human
  • Entrez Gene: 12647 Mouse
  • Entrez Gene: 396896 Pig
  • Entrez Gene: 290567 Rat
  • Omim: 118490 Human
  • SwissProt: P28329 Human
  • SwissProt: Q03059 Mouse
  • SwissProt: P13222 Pig

  • SwissProt: P32738 Rat
  • Unigene: 302002 Human
  • Unigene: 442817 Mouse
  • Unigene: 45116 Rat

  see all

• Alternative names
  Acetyl CoA choline O acetyltransferase antibodyAcetyl CoA:choline O acetyltransferase antibodyChAT antibody

  CHOACTase antibodyCholine acetylase antibodycholine acetyltransferase antibodyCholine O acetyltransferase antibodyCholine O acetyltransferase antibodyCholine O-acetyltransferase antibodyCLAT_HUMAN antibodyCMS1A antibodyCMS1A2 antibodyEC 2.3.1.6 antibodyOTTHUMP00000019583 antibodyOTTHUMP00000019584 antibody

  see all

Anti-Choline Acetyltransferase antibody images

• 

  Immunocytochemistry/ Immunofluorescence - Choline Acetyltransferase antibody (ab68779)
  ICC/IF image of ab68779 stained PC12 cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab68779, 1µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.