Anti-Choline Acetyltransferase antibody (ab68779)

Overview

  • Product nameAnti-Choline Acetyltransferase antibodySee all Choline Acetyltransferase primary antibodies ...
  • Description
    Rabbit polyclonal to Choline Acetyltransferase
  • SpecificitySpecific for ChAT.
  • Tested applicationsWB, IHC-P, IHC-Fr, ICC/IF more details
  • Species reactivity
    Reacts with: Mouse, Rat, Guinea pig, Dog, Human, Pig
  • Immunogen

    A synthetic peptide as a part of human, rat and mouse Choline Acetyltransferase conjugated to an immunogenic carrier protein.

Properties

Applications

Our Abpromise guarantee covers the use of ab68779 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Notes
WB Use a concentration of 1 - 10 µg/ml. This antibody has been tested in Western blot against the recombinant peptide used as an immunogen. We have no data on detection of endogenous protein.
IHC-P Use a concentration of 1 - 10 µg/ml. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
IHC-Fr Use a concentration of 1 - 10 µg/ml.
ICC/IF Use a concentration of 1 µg/ml.

Target

  • FunctionCatalyzes the reversible synthesis of acetylcholine (ACh) from acetyl CoA and choline at cholinergic synapses.
  • Involvement in diseaseDefects in CHAT are the cause of congenital myasthenic syndrome with episodic apnea (CMSEA) [MIM:254210]; formerly known as familial infantile myasthenia gravis 2 (FIMG2). CMSEA is an autosomal recessive congenital myasthenic syndrome. Patients have myasthenic symptoms since birth or early infancy, negative tests for anti-AChR antibodies, and abrupt episodic crises with increased weakness, bulbar paralysis, and apnea precipitated by undue exertion, fever, or excitement.
  • Sequence similaritiesBelongs to the carnitine/choline acetyltransferase family.
  • Target information above from: UniProt accession P28329 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Database links
  • Alternative names
    • Acetyl CoA choline O acetyltransferase antibody
    • Acetyl CoA:choline O acetyltransferase antibody
    • ChAT antibody
    • CHOACTase antibody
    • Choline acetylase antibody
    • choline acetyltransferase antibody
    • Choline O acetyltransferase antibody
    • Choline O acetyltransferase antibody
    • Choline O-acetyltransferase antibody
    • CLAT_HUMAN antibody
    • CMS1A antibody
    • CMS1A2 antibody
    • EC 2.3.1.6 antibody
    • OTTHUMP00000019583 antibody
    • OTTHUMP00000019584 antibody
    see all

Anti-Choline Acetyltransferase antibody images

  • ICC/IF image of ab68779 stained PC12 cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab68779, 1µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) ab150077) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI (ab104139) was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

References for Anti-Choline Acetyltransferase antibody (ab68779)

This product has been referenced in:
  • Kitiyanant N  et al. BDNF-, IGF-1- and GDNF-Secreting Human Neural Progenitor Cells Rescue Amyloid ß-Induced Toxicity in Cultured Rat Septal Neurons. Neurochem Res 37:143-52 (2012). WB ; Rat . Read more (PubMed: 21909955) »
  • Garbuzova-Davis S  et al. Multiple intravenous administrations of human umbilical cord blood cells benefit in a mouse model of ALS. PLoS One 7:e31254 (2012). IHC-Fr ; Mouse . Read more (PubMed: 22319620) »

See all 8 Publications for this product

Product Wall

I recommend starting with a dilution of 10ug/ml, which is 1/100 if using the stock 1mg/ml antibody solution you receive, or 1/50 if you dilute what you receive with glycerol to a final percentage of 50% glycerol. You may want to test a series of diluti...

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Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Antigen retrieval step Heat mediated - Buffer/Enzyme Used: Citric acid
Sample Mouse Tissue sections (Brain)
Specification Brain
Blocking step BSA as blocking agent for 21 minute(s) · Concentration: 1% · Temperature: 21°C
Fixative Formaldehyde
Username

Mr. Carl Hobbs

Verified customer

Submitted Nov 04 2013

Thank you for contacting us. I hope that the suggestions which were made help you to resolve the issues which you have been experiencing. As requested, I have arranged for you to receive positive control lysates ab71719 and ab29330 free of charge. T...

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Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample Dog Tissue sections (Peripheral Nerve)
Specification Peripheral Nerve
Fixative Formaldehyde
Antigen retrieval step Enzymatic - Buffer/Enzyme Used: Prot K
Permeabilization No
Blocking step Background Buster as blocking agent for 30 minute(s) · Concentration: 100% · Temperature: RT°C
Username

Abcam user community

Verified customer

Submitted Mar 02 2012

Thanks a lot for clarifying.
As a negative control for your IHC assay, I would recommend using an isotype control, an antibody from the same species and isotype as the primary with does not target any specific protein.
I conducted a search fo...

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Thank you for your enquiry.

As glycerol is a cryoprotectant, if the antibody has been frozen for a few months without it, it may regrettablyalready be damaged.

However, I would suggest to try one aliquot of each antibody in an exp...

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Thank you for your reply.


When you add glycerol to a final volume of 50%, it means that half of the total finalvolume should be antibody and half of the volume should be glycerol. Therefore, if the antibody is 100ul, you should add 100u...

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DISCOUNT CODES:
ab68779 - **********
ab14184 - ***********
ab65832 - ******************
ab7349 - ***************

Expiration date fro all discount codes : *********

I am pleased to hear you would like to accept ...

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Thank you for providing the images and details of the our customer's experiments.

While we do not test this antibody for use on perfused-fixed frozen sections (IHC-FoFr), it is possible that with optimization it may be suitable for this purpo...

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Thank you very much for your interest in our products. The following antibodies are tested and guaranteed for IHC-P in rat. Unfortunately, we do not have blocking peptide for these antibodies in our catalogue. ab68779 Click here (or use...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"