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A synthetic peptide as a part of human, rat and mouse Choline Acetyltransferase conjugated to an immunogenic carrier protein.
Our Abpromise guarantee covers the use of ab68779 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||Use a concentration of 1 - 10 µg/ml. Can be blocked with Choline Acetyltransferase peptide (ab203374). This antibody has been tested in Western blot against the recombinant peptide used as an immunogen. We have no data on detection of endogenous protein.|
|IHC-P||Use a concentration of 1 - 10 µg/ml. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.|
|IHC-Fr||Use a concentration of 1 - 10 µg/ml.|
|ICC/IF||Use a concentration of 1 µg/ml.|
ICC/IF image of ab68779 stained PC12 cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab68779, 1µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) ab150077) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI (ab104139) was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
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