Overview
- Product nameAnti-Choline Acetyltransferase antibodySee all Choline Acetyltransferase primary antibodies ...
- DescriptionRabbit polyclonal to Choline Acetyltransferase
- Tested applicationsICC/IF, IHC-P, IHC-Fr, WB more details
- Species reactivityReacts with: Mouse, Rat
- Immunogen
Synthetic peptide as a part of rat and mouse ChAT (choline O-acetyltransferase) conjugated to an immunogenic carrier protein.
- Positive control1 day old mouse cortical cell culture Rat spinal cord
Properties
- FormLiquid
- Storage instructionsStore at +4°C short term (1-2 weeks). Add glycerol to a final volume of 50%, aliquot and store at -20°C. Avoid repeated freeze / thaw cycles.
- Storage bufferPreservative: None
Constituents: Whole serum - PurityWhole antiserum
- Clonality Polyclonal
- IsotypeIgG
- Research Areas
Applications
Our Abpromise guarantee covers the use of ab70219 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Notes |
|---|---|
| ICC/IF | |
| IHC-P | |
| IHC-Fr | |
| WB |
IHC-P: 1/300 - 1/2000. Antigen retrieval is recommended.
IHC-Fr: 1/300 - 1/2000.
WB: 1/300 - 1/2000. Predicted molecular weight: 83 kDa.
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
Target
- FunctionCatalyzes the reversible synthesis of acetylcholine (ACh) from acetyl CoA and choline at cholinergic synapses.
- Involvement in diseaseDefects in CHAT are the cause of congenital myasthenic syndrome with episodic apnea (CMSEA) [MIM:254210]; formerly known as familial infantile myasthenia gravis 2 (FIMG2). CMSEA is an autosomal recessive congenital myasthenic syndrome. Patients have myasthenic symptoms since birth or early infancy, negative tests for anti-AChR antibodies, and abrupt episodic crises with increased weakness, bulbar paralysis, and apnea precipitated by undue exertion, fever, or excitement.
- Sequence similaritiesBelongs to the carnitine/choline acetyltransferase family.
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Database links
- Entrez Gene: 12647 Mouse
- Entrez Gene: 290567 Rat
- SwissProt: Q03059 Mouse
- SwissProt: P32738 Rat
- Unigene: 442817 Mouse
- Unigene: 45116 Rat
Target information above from: UniProt accession
P28329
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010)
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Alternative names
- Acetyl CoA choline O acetyltransferase antibodyAcetyl CoA:choline O acetyltransferase antibodyChAT antibody
- CHOACTase antibodyCholine acetylase antibodycholine acetyltransferase antibodyCholine O acetyltransferase antibodyCholine O acetyltransferase antibodyCholine O-acetyltransferase antibodyCLAT_HUMAN antibodyCMS1A antibodyCMS1A2 antibodyEC 2.3.1.6 antibodyOTTHUMP00000019583 antibodyOTTHUMP00000019584 antibody
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Anti-Choline Acetyltransferase antibody images
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Immunocytochemistry/ Immunofluorescence - Choline Acetyltransferase antibody (ab70219)Immunofluorescence analysis of 1 day old mouse cortical cell culture using ab70219 at 1/2000 dilution. Cells are counterstained with DAPI. -
Immunocytochemistry/ Immunofluorescence - Choline Acetyltransferase antibody (ab70219)Immunofluorescence analysis of 1 day old mouse cortical cell culture using ab70219 at 1/2000 dilution. Cells are counterstained with DAPI. -
- Choline Acetyltransferase antibody (ab70219)Immunohistochemical analysis of rat spinal cord using ab70219 at 1/2000 dilution. -
- Choline Acetyltransferase antibody (ab70219)Immunohistochemical analysis of rat spinal cord using ab70219 at 1/2000 dilution. -
- Choline Acetyltransferase antibody (ab70219)Immunohistochemical analysis of rat spinal cord using ab70219 at 1/2000 dilution.
References for Anti-Choline Acetyltransferase antibody (ab70219)
ab70219 has not yet been referenced specifically in any publications.
