Overview

  • Product nameCholine/Acetylcholine Assay Kit
  • Tests
    100 x 1 assay
  • Sample type
    Cell culture supernatant, Urine, Serum, Plasma, Cell culture extracts, Other biological fluids, Tissue Extracts
  • Assay typeQuantitative
  • Sensitivity
    > 0.01 nmol/well
  • Range
    0.01 nmol/well - 5 nmol/well
  • Assay time
    0h 40m
  • Species reactivity
    Reacts with: Mouse, Human
    Predicted to work with: all Mammals
  • Product overview

    Abcam's Choline/Acetylcholine Assay Kit provides a simple and sensitive means for quantifying Choline and Acetylcholine by either a colorimetric or fluorometric method. In the assay free choline is oxidized to betaine, via the intermediate betaine aldehyde. The reaction generates products which react with the Choline Probe to generate color (λ= 570 nm), and fluorescence (Ex/Em 535/587 nm). Acetylcholine can be converted to choline by adding acetylcholinesterase to the reaction. The kit can detect choline and acetylcholine (total choline – free choline) in various biological samples such as in blood, cells, culture media, fermentation media, etc. There is no need for pretreatment or purification of samples. The kit can detect ~10 pmol-5 nmol of choline or acetylcholine.


    Visit our FAQs page for tips and troubleshooting.

  • Notes

    Choline and acetylcholine play important roles in many biological processes. Choline is an essential nutrient, commonly grouped with the B complex vitamins, that plays key roles in many biological processes. Choline is a precursor for the synthesis of acetylcholine (AchE), a critical neurotransmitter in both the periferial and central nervous systems.

     

     

    If you need to purchase additional Choline Probe for this product, please see ab139505.

  • Tested applicationsFunctional Studiesmore details

Properties

  • RelevanceCholine and acetylcholine (often abbreviated ACh) play important roles in many biological processes. Choline is a natural amine, and is generally found in phosphatidylcholine and sphingomyelin, phopholipids abundant in cell membranes. Choline is also the precursor molecule for the neurotransmitter acetylcholine which is involved in many functions both in the peripheral nervous system (PNS) and in the central nervous system (CNS). In the PNS, ACh activates muscles and is a major neurotransmitter in the autonomic nervous system. In the CNS, ACh and the associated neurons form the cholinergic system, which tends to cause anti-excitatory actions.

Applications

Our Abpromise guarantee covers the use of ab65345 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Functional Studies Use at an assay dependent dilution.

Choline/Acetylcholine Assay Kit images

  • Choline measured in mouse tissue lysates showing quantity (nmol) per mg protein of tested sample

  • Choline measured in cell lysates showing quantity (pmol) per 1 mln of tested cells

  • Choline measured in biological fluids showing quantity (nmol) per mL of tested sample

  • Standard curve (colourimetric) : mean of duplicates (+/-SD) with background readings substracted

  • Choline/Acetylcholine Standard Curve (colorimetric reading): Assays were performed following the kit instructions. The diamonds are generated using choline as the substrate, whereas the open and closed circles were generated using acetylcholine as substrate in the presence and absence of acetylcholinesterase.

Protocols

References for Choline/Acetylcholine Assay Kit (ab65345)

This product has been referenced in:
  • Harvey KL  et al. Ipratropium bromide-mediated myocardial injury in in vitro models of myocardial ischaemia/reperfusion. Toxicol Sci 138:457-67 (2014). Read more (PubMed: 24431217) »
  • Kim JH  et al. Electroacupuncture acutely improves cerebral blood flow and attenuates moderate ischemic injury via an endothelial mechanism in mice. PLoS One 8:e56736 (2013). Functional Studies ; Mouse . Read more (PubMed: 23418594) »

See all 4 Publications for this product

Product Wall

I suggest running a time course to see how much time is needed for your samples to show a linear increase and then plateau off. It is recommended to choose the final incubation time within this linear increase regime.
Also, please note it is impor...

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ab65345 cannot be used for in-cell assay. It is meant for cell lysates.

Thank you for your email and your interest in our products.

I can confirm that you are right. It will be necessary to set up 2 different sets of wells for measuring the total choline and the free choline in this assay.

Therefore, to m...

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Thank you for contacting us.

We have not tested this kit with rat samples. However this kit should work as it does not contain any human specific antibody. The species is irrelevant for this product.

It will also be a good idea to inc...

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Thank you for contacting us.

All of the information and images we have for each of our products is listed on the online datasheet for your convenience. These are updated as soon as any new information is brought to our attention. Unfortunatel...

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Thank you very much for contacting Abcam. Your details has been forwarded to me by my colleague Peter.

The kit ab65345 have a detection range of 0.01 nmol/well to 5 nmol/ml; expression outside of this range will be undetectablewith the kit. <...

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Thank you for pointing this out. The datasheet is somewhat mis-leading with regards to the 500-fold range that is specified, and also in error with regards to the notation. I apologize for the confusion.

The specified range uses the low valu...

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Tissue culture SN can be used with this assay. Add up to 50 µl of the SN to wells and make up the final volume in all wells to 50 µl with the assay buffer. Continue with the protocol.

Thank you for your email. I am sorry to hear that you have been experiencing problems with this kit.

I have checked the details you have kindly provided; however I would like to ask few questions in order to understand the problem;

...

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Thank you for your inquiry.

I can confirm that ab65345 should be suitable for your sample (Krebs solution after anorgan bath).

This has not been experimentally tested by the lab and therefore might requiry some optimisation. The kit...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"