You have changed your country from  to  . Please be aware that this will change the currency in the purchasing process.

Overview

  • Product nameCholine/Acetylcholine Assay Kit
  • Tests
    100 x 1 assay
  • Sample type
    Cell culture supernatant, Urine, Serum, Plasma, Other biological fluids, Tissue Extracts
  • Assay typeQuantitative
  • Sensitivity
    > 0.01 nmol/well
  • Range
    0.01 nmol/well - 5 nmol/well
  • Assay time
    0h 40m
  • Product overview

    Abcam's Choline/Acetylcholine Assay Kit provides a simple and sensitive means for quantifying Choline and Acetylcholine by either a colorimetric or fluorometric method. In the assay free choline is oxidized to betaine, via the intermediate betaine aldehyde. The reaction generates products which react with the Choline Probe to generate color (λ= 570 nm), and fluorescence (Ex/Em 535/587 nm). Acetylcholine can be converted to choline by adding acetylcholinesterase to the reaction. The kit can detect choline and acetylcholine (total choline – free choline) in various biological samples such as in blood, cells, culture media, fermentation media, etc. There is no need for pretreatment or purification of samples. The kit can detect ~10 pmol-5 nmol of choline or acetylcholine.
    Visit our FAQs page for tips and troubleshooting.

  • Notes

    Choline and acetylcholine play important roles in many biological processes.

  • Tested applicationsFunctional Studies more details

Properties

  • RelevanceCholine and acetylcholine (often abbreviated ACh) play important roles in many biological processes. Choline is a natural amine, and is generally found in phosphatidylcholine and sphingomyelin, phopholipids abundant in cell membranes. Choline is also the precursor molecule for the neurotransmitter acetylcholine which is involved in many functions both in the peripheral nervous system (PNS) and in the central nervous system (CNS). In the PNS, ACh activates muscles and is a major neurotransmitter in the autonomic nervous system. In the CNS, ACh and the associated neurons form the cholinergic system, which tends to cause anti-excitatory actions.

    • Applications

    Our Abpromise guarantee covers the use of ab65345 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    Application Notes
    Functional Studies FuncS

    Choline/Acetylcholine Assay Kit images

    • Functional Studies - Choline/Acetylcholine Assay Kit (ab65345)
      Functional Studies - Choline/Acetylcholine Assay Kit (ab65345)
      Choline/Acetylcholine Assays were performed following the kit instructions. The diamonds are generated using choline as the substrate, whereas the open and closed circles were generated using acetylcholine as substrate in the presence and absence of acetylcholinesterase.

    Protocols

    References for Choline/Acetylcholine Assay Kit (ab65345)

    This product has been referenced in:
    • Milara J  et al. Aclidinium inhibits human lung fibroblast to myofibroblast transition. Thorax 67:229-37 (2012). Read more (PubMed: 21957094) »
    • Reale M  et al. Relation between Pro-inflammatory Cytokines and Acetylcholine Levels in Relapsing-Remitting Multiple Sclerosis Patients. Int J Mol Sci 13:12656-64 (2012). Read more (PubMed: 23202919) »

    See all 2 Publications for this product

    Publishing research using ab65345 ? Please let us know so that we can cite the reference in this datasheet

    Product Wall

    Submit a Question for ab65345

    Displaying 1 - 10 of 15 results for Q&A

    10
    Votes: Highest First

    Can this kit be used for an in-cell assay? Can the probe permeabilize the membrane of live cells?

    ab65345 cannot be used for in-cell assay. It is meant for cell lysates.

    We want to know the amount of acetylcholine in our plasma samples. If the assay measures total choline when acetylcholinesterase is added, how do we know the amount of free choline to ultimately determine acetylcholine (ACh = total choline - free choline...

    Read More

    Thank you for your email and your interest in our products.

    I can confirm that you are right. It will be necessary to set up 2 different sets of wells for measuring the total choline and the free choline in this assay.

    Therefore, to meas...

    Read More

    I am about to embark into a project looking for stroke biomarker in MCA occluded rat- I intend to use your product choline acetylcholine assay kit - As my study is on rat, I want to know how much of homology is their between the human and rat protein. Wi...

    Read More

    Thank you for contacting us.

    We have not tested this kit with rat samples. However this kit should work as it does not contain any human specific antibody. The species is irrelevant for this product.

    It will also be a good idea to includ...

    Read More

    Dear All,
    a customer of ours would like to know if you have any reference concerning the use of your AB65345on rat synaptosomes.
    Thanks in advance and kind regards

    Thank you for contacting us.

    All of the information and images we have for each of our products is listed on the online datasheet for your convenience. These are updated as soon as any new information is brought to our attention. Unfortunately w...

    Read More

    Thank you for the follow up. Unfortunately, we have performed some troubleshooting on the methodology but it seems that our cholinergic neuroblastomas do not release sufficient amounts of acetylcholine to be detected by your kit.Best regards

    Thank you very much for contacting Abcam. Your details has been forwarded to me by my colleague Peter.

    The kit ab65345 have a detection range of 0.01 nmol/well to 5 nmol/ml; expression outside of this range will be undetectablewith the kit.
    Read More

    I'm trying to estimate whether your kit can detect the amounts of ACh/Ch that I expect to find in our samples. So, just to clarify, it appears that (starting from your graph) one can have as much as 5000 picomoles of Ch in a well. And 50 ul of that volume...

    Read More

    Thank you for pointing this out. The datasheet is somewhat mis-leading with regards to the 500-fold range that is specified, and also in error with regards to the notation. I apologize for the confusion.

    The specified range uses the low value f...

    Read More

    Can the customer use Tissue culture SN? If so, how is the sample preparation?

    Tissue culture SN can be used with this assay. Add up to 50 µl of the SN to wells and make up the final volume in all wells to 50 µl with the assay buffer. Continue with the protocol.

    To whom this may concern,
    We have recently procured the choline assay kit from your company for a second time. However, except for the first attempt at using the first kit, we have seen that the standard curves (most likely the measured samples as we...

    Read More

    Thank you for your email. I am sorry to hear that you have been experiencing problems with this kit.

    I have checked the details you have kindly provided; however I would like to ask few questions in order to understand the problem;

    - C...

    Read More

    To whom it may concern,

    I would like to ask you an information about your product Choline/Acetylcholine Assay Kit (cat no. ab65345). Could it be used to determine the acetylcholine content in sample of Krebs solution obtained by organ baths in ...

    Read More

    Thank you for your inquiry.

    I can confirm that ab65345 should be suitable for your sample (Krebs solution after anorgan bath).

    This has not been experimentally tested by the lab and therefore might requiry some optimisation. The kit ca...

    Read More

    Hi!

    Thank you for your goodwill. I hope that we can fix the problem of the kit by using premade standard. Apart from this the results look reliable. It would be best if you could provide me a new kit. Its best to send it to:

    ####

    Read More

    Thank you for confirming these details and for your cooperation. The details provided enable us to closely monitor the quality of our products.

    I am sorry this product did not perform as stated on the datasheet and for the inconvenience this ha...

    Read More
    

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"