Anti-Chromogranin A antibody [CHGA/798] (ab220191)
Key features and details
- Mouse monoclonal [CHGA/798] to Chromogranin A
- Suitable for: IHC-P
- Reacts with: Mouse, Rat, Human
- Isotype: IgG1
Overview
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Product name
Anti-Chromogranin A antibody [CHGA/798]
See all Chromogranin A primary antibodies -
Description
Mouse monoclonal [CHGA/798] to Chromogranin A -
Host species
Mouse -
Tested applications
Suitable for: IHC-Pmore details -
Species reactivity
Reacts with: Mouse, Rat, Human -
Immunogen
Full length protein corresponding to Human Chromogranin A.
Database link: P10645 -
Positive control
- IHC: Human SC lung carcinoma, pancreas, parathyroid, adrenal gland tissues. Rat pancreas. Mouse pancreas.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.2
Preservative: 0.05% Sodium azide
Constituents: 0.05% BSA, 99% PBS -
Concentration information loading...
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Purity
Protein A/G purified -
Purification notes
ab220191 was purified from Bioreactor Concentrate by Protein A/G. -
Clonality
Monoclonal -
Clone number
CHGA/798 -
Isotype
IgG1 -
Light chain type
kappa -
Research areas
Associated products
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Alternative Versions
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Compatible Secondaries
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Isotype control
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Recombinant Protein
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Related Products
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab220191 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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IHC-P |
Use a concentration of 0.25 - 0.5 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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Notes |
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IHC-P
Use a concentration of 0.25 - 0.5 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
Target
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Function
Pancreastatin strongly inhibits glucose induced insulin release from the pancreas. -
Sequence similarities
Belongs to the chromogranin/secretogranin protein family. -
Post-translational
modificationsSulfated on tyrosine residues and/or contains sulfated glycans.
O-glycosylated with core 1 or possibly core 8 glycans. -
Cellular localization
Secreted. Neuroendocrine and endocrine secretory granules. - Information by UniProt
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Database links
- Entrez Gene: 1113 Human
- Entrez Gene: 12652 Mouse
- Entrez Gene: 24258 Rat
- Omim: 118910 Human
- SwissProt: P10645 Human
- SwissProt: P26339 Mouse
- SwissProt: P10354 Rat
- Unigene: 150793 Human
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Form
According to the Swiss-Prot database, this protein has many different forms which correspond to various molecular weights; thus the possible variation from the predicted molecular weight of 51kDa. -
Alternative names
- beta Granin antibody
- betagranin (N-terminal fragment of chromogranin A) antibody
- catestatin antibody
see all
Images
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of mouse pancreas tissue labelling Chromagranin A with ab220191 at 0.5µg/ml dilution.
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Immunohistochemical analysis of formalin-fixed and paraffin-embedded Human adrenal gland tissue labeling Chromogranin A with ab220191 at 0.5 μg/ml dilution.
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Immunohistochemical analysis of formalin-fixed and paraffin-embedded Human parathyroid tissue labeling Chromogranin A with ab220191 at 0.5 μg/ml dilution.
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Immunohistochemical analysis of formalin-fixed and paraffin-embedded rat pancreas tissue labeling Chromogranin A with ab220191 at 0.5 μg/ml dilution.
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Immunohistochemical analysis of formalin-fixed and paraffin-embedded Human pancreas tissue labeling Chromogranin A with ab220191 at 0.5 μg/ml dilution.
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Immunohistochemical analysis of formalin-fixed and paraffin-embedded Human SC lung carcinoma tissue labeling Chromogranin A with ab220191 at 0.5 μg/ml dilution.
Datasheets and documents
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Datasheet download
References (0)
ab220191 has not yet been referenced specifically in any publications.