![Western blot - Cig2 antibody [CIG 3A11/5] (ab10881) image](#ab10881_1.jpg)
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Read our guarantee »Overview
Product name
Anti-Cig2 antibody [CIG 3A11/5]
Description
Mouse monoclonal [CIG 3A11/5] to Cig2
Tested applications
WB, ELISA, IPmore details
Cross reactivity
Reacts with
Schizosaccharomyces pombe
Immunogen
Bacterial S. pombe Cig2 purified protein.
Properties
Form
Liquid
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Storage buffer
Preservative: None
Constituents: PBS
Concentration
Concentration information loading...
Purity
Protein A purified
Clonality
Monoclonal
Clone number
CIG 3A11/5
Myeloma
Sp2
Isotype
IgG1
Research areas
Microbiology >> Organism >> Eukaryotic Microorganism >> Yeast >> Other Yeast species
Cell Biology >> Cell Cycle >> Cyclins >> Cyclin B Family
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Western blot - Cig2 antibody [CIG 3A11/5] (ab10881)
![Western blot - Cig2 antibody [CIG 3A11/5] (ab10881) image](/ps/datasheet/Images/10/ab10881/ab10881_1.jpg)
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Applications
Show applications key
Our Abpromise guarantee covers the use of ab10881 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
WB: 1/1000.
ELISA: Use at an assay dependent dilution.
IP: Use at an assay dependent dilution.
Target
Relevance
Cig2 is a fission yeast (Schizosaccharomyces pombe) B-type S-phase cyclin and is expressed in a sharp spike peaking at G1/S period. Cig2 binds to Cdc2 (CDK), and the resulting Cdc2-Cig2 complex controls the G1/S transition of the cell cycle.
Anti-Cig2 antibody [CIG 3A11/5] images:
Western blot - Cig2 antibody [CIG 3A11/5] (ab10881)
All lanes : Anti-Cig2 antibody [CIG 3A11/5] (ab10881) at 1/1000 dilution
Lane 1 : 20ug untreated S. pombe wildtype cells, whole cell lysate
Lane 2 : 20ug S. pombe wildtype cells 8mM HU treated for 1 hour, whole cell lysate
Lane 3 : 20ug S. pombe wildtype cells 8mM HU treated for 2 hours, whole cell lysate
Lane 4 : 20ug S. pombe wildtype cells 8mM HU treated for 3 hours, whole cell lysate
Secondary
HRP conjugated sheep anti-mouse antibody
developed using the ECL technique
Observed band size : 50 kDa (why is the actual band size different from the predicted?)
Exposure time : 1 minute
This image is courtesy of an anonymous Abreview
References for Anti-Cig2 antibody [CIG 3A11/5] (ab10881)
This product has been referenced in:
- Chu Z et al. A Cds1-mediated checkpoint protects the MBF activator Rep2 from ubiquitination by anaphase-promoting complex/cyclosome-Ste9 at S-phase arrest in fission yeast. Mol Cell Biol 29:4959-70 (2009).Read more (PubMed: 19596787) »
- Chu Z et al. Modulation of cell cycle-specific gene expressions at the onset of S phase arrest contributes to the robust DNA replication checkpoint response in fission yeast. Mol Biol Cell 18:1756-67 (2007).Read more (PubMed: 17332498) »
See all 3 publications for this product
Publishing research using ab10881? Please let us know so that we can cite the reference in this datasheet
![Cig2 antibody [CIG 3A11/5] for WB in Schizosaccharomyces pombe (10881)](/ps/Reviews/Images/ab5569_19323.jpg)
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"