Overview

  • Product nameCitrate Assay Kit
  • Tests
    100 x 1 assay
  • Sample type
    Cell culture supernatant, Urine, Serum, Plasma, Other biological fluids, Tissue Extracts
  • Assay typeQuantitative
  • Sensitivity
    > 0.002 mM
  • Range
    0.002 mM - 10 mM
  • Assay time
    0h 40m
  • Product overview

    Abcam's Citrate Assay Kit provides a simple, sensitive and rapid means of quantifying citrate in a variety of samples. In the assay, citrate is converted to pyruvate via oxaloacetate. The pyruvate is quantified by converting a nearly colorless probe to an intensely colored (570 nm) and fluorescent (Ex/Em, 535/587 nm) product. The Citrate Assay Kit can detect 0.1 to 10 nmoles (~2 µM-10 mM) of citrate in a variety of samples.
    Visit our FAQs page for tips and troubleshooting.

  • Notes

    Citric acid (HOOC-CH2-C(-OH)(-COOH)-CH2-COOH) is a key intermediate in the TCA cycle which occurs in mitochondria. It is formed by the addition of oxaloacetate to the acetyl group of acetyl-CoA derived from the glycolytic pathway. Citrate can be transported out of mitochondria and converted back to acetyl CoA for fatty acid synthesis. Citrate is an allosteric modulator of both fatty acid synthesis (acetyl-CoA carboxylase) and glycolysis (phospho- fructokinase). Citrate is widely used industrially in foods, beverages and pharmaceuticals. Citrate metabolism and disposition can vary widely due to sex, age and a variety of other factors.

  • Tested applicationsFunctional Studiesmore details

Properties

  • RelevanceCitric acid (HOOC-CH2-C(-OH)(-COOH)-CH2-COOH) is a key intermediate in the TCA cycle which occurs in mitochondria. It is formed by the addition of oxaloacetate to the acetyl group of acetyl-CoA derived from the glycolytic pathway. Citrate can be transported out of mitochondria and converted back to acetyl CoA for fatty acid synthesis. Citrate is an allosteric modulator of both fatty acid synthesis (acetyl-CoA carboxylase) and glycolysis (phospho-fructokinase). Citrate is widely used industrially in foods, beverages and pharmaceuticals. Citrate metabolism and disposition can vary widely due to sex, age and a variety of other factors.
  • Alternative names
    • Citric acid

Applications

Our Abpromise guarantee covers the use of ab83396 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Functional Studies Use at an assay dependent dilution.

Citrate Assay Kit images

  • Citrate standard curve generated using this kit protocol

Protocols

References for Citrate Assay Kit (ab83396)

This product has been referenced in:
  • Elmaci AM  et al. Clinical characteristics and metabolic abnormalities in preschool-age children with urolithiasis in southeast Anatolia. J Pediatr Urol N/A:N/A (2013). Read more (PubMed: 24314604) »

See 1 Publication for this product

Product Wall

Urine is a liquid and therefore doesn't need to be homogenised. It is true that enzymes in samples may interfere with the assay which is why we suggest deproteinizing samples using a perchloric acid/KOH protocol or 10 kd molecular weight cut off spin c...

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The lab has confirmed that the probe and all the reconstituted lyophilized components can be stored in aliquots at-20C. If never frozen and thawed, it is possible that the components will still work beyond 2 months.


The Urine can be store...

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This kit can detect 0.1 to 10 nmoles (˜2 µM-10 mM) of citrate in a variety of samples. It can detect sodium citrate. In fact that is used as the standard.

Thank you for your enquiry.
The optimum dilution factor depends on the cell type, the concentration of citrate in the cells and the overall quality of the lysate. This will need to be individually determined.
I would recommend making a series...

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We suggest testing several doses of your samples to ensure readings are within the standard curve range. The minimum dilution that we normally recommend for testing serum and plasma samples is 2-fold. Running these...

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Thank you for contacting us and your interest in our products.

I am sorry but I am not sure of the property you are seeking. I am not familiar with the "CoV". In order to help further in your query, could you please clarify this furt...

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Thanks for your reply and your suggestions. I'll be sure to pass them on to the lab.

Please let me know if you have any further questions.

The orbital shaker is not requiredto run this assay.Sorry for the confusion. You could, however, use it when incubating reagents in the microtiter plate if you wish but this is not required.

We can likely make the assay buffer available separ...

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Thank you for your enquiry.

I am sorry to confirm this kit has not yet been tested with bone samples, so we would not be certain how it would work. I would suggest that the protocol can be followed from the deproteinizing stage.

H...

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Thank you for contacting us.

The assay buffers in each of these kits is different. The buffers are optimized in order to get the best results. Please use the assay buffer for the sample, specific to each kit.

I hope this informati...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"