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Overview

  • Product nameCitrate Assay Kit
  • Tests
    100 x 1 assay
  • Sample type
    Cell culture supernatant, Urine, Serum, Plasma, Other biological fluids, Tissue Extracts
  • Assay typeQuantitative
  • Sensitivity
    > 0.002 mM
  • Range
    0.002 mM - 10 mM
  • Assay time
    0h 40m
  • Product overview

    Abcam's Citrate Assay Kit provides a simple, sensitive and rapid means of quantifying citrate in a variety of samples. In the assay, citrate is converted to pyruvate via oxaloacetate. The pyruvate is quantified by converting a nearly colorless probe to an intensely colored (570 nm) and fluorescent (Ex/Em, 535/587 nm) product. The Citrate Assay Kit can detect 0.1 to 10 nmoles (~2 µM-10 mM) of citrate in a variety of samples.
    Visit our FAQs page for tips and troubleshooting.

  • Notes

    Citric acid (HOOC-CH2-C(-OH)(-COOH)-CH2-COOH) is a key intermediate in the TCA cycle which occurs in mitochondria. It is formed by the addition of oxaloacetate to the acetyl group of acetyl-CoA derived from the glycolytic pathway. Citrate can be transported out of mitochondria and converted back to acetyl CoA for fatty acid synthesis. Citrate is an allosteric modulator of both fatty acid synthesis (acetyl-CoA carboxylase) and glycolysis (phospho- fructokinase). Citrate is widely used industrially in foods, beverages and pharmaceuticals. Citrate metabolism and disposition can vary widely due to sex, age and a variety of other factors.

  • Tested applicationsFunctional Studies more details

Properties

  • RelevanceCitric acid (HOOC-CH2-C(-OH)(-COOH)-CH2-COOH) is a key intermediate in the TCA cycle which occurs in mitochondria. It is formed by the addition of oxaloacetate to the acetyl group of acetyl-CoA derived from the glycolytic pathway. Citrate can be transported out of mitochondria and converted back to acetyl CoA for fatty acid synthesis. Citrate is an allosteric modulator of both fatty acid synthesis (acetyl-CoA carboxylase) and glycolysis (phospho-fructokinase). Citrate is widely used industrially in foods, beverages and pharmaceuticals. Citrate metabolism and disposition can vary widely due to sex, age and a variety of other factors.
    • Alternative names
        Citric acid

    Applications

    Our Abpromise guarantee covers the use of ab83396 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    Application Notes
    Functional Studies FuncS

    Citrate Assay Kit images

    • Functional Studies - Citrate Assay Kit (ab83396)
      Functional Studies - Citrate Assay Kit (ab83396)
      Citrate standard curve generated using this kit protocol

    Protocols

    References for Citrate Assay Kit (ab83396)

    ab83396 has not yet been referenced specifically in any publications.

    Publishing research using ab83396 ? Please let us know so that we can cite the reference in this datasheet

    Product Wall

    Submit an Abreview for ab83396 Submit a Question for ab83396

    Displaying 1 - 10 of 15 results for Abreviews and Q&A

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    I would like to ask you a couple of questions about the assay protocol.

    It doesn’t say anything about plasma samples. What is the dilution, do they need to be deproteinised? My samples are mouse plasma collected in heparinised syringes and ...

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    Thank you for contacting us.

    We suggest testing several doses of your samples to ensure readings are within the standard curve range. The minimum dilution that we normally recommend for testing serum and plasma samples is 2-fold. Running these sa...

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    Product code: 83396
    Inquiry: Hi, I was wondering if yo knew what the CoV for this product is

    Thank you for contacting us and your interest in our products.

    I am sorry but I am not sure of the property you are seeking. I am not familiar with the "CoV". In order to help further in your query, could you please clarify this further...

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    Thank you for your clarification at length. The information is quite helpful along w the link on the dounce for small volumes.



    In future, if we have to buy more kits and run samples, will request you to enable its purchase indi...

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    Thanks for your reply and your suggestions. I'll be sure to pass them on to the lab.

    Please let me know if you have any further questions.

    I have some questions about this kit.
    1. Protocol states an orbital shaker will be required but I don't see it mentioned in the protocol. When is it used?
    2. Is the Assay sample buffer available as a separate reagent? Or is the recipe available?...

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    The orbital shaker is not requiredto run this assay.Sorry for the confusion. You could, however, use it when incubating reagents in the microtiter plate if you wish but this is not required.

    We can likely make the assay buffer available separate...

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    We want to use ab83396 citrate assay kit on bone samples prepared with HCL.
    Is it possible to use this kit?
    Can we have a free sample to try?

    Thank you for your enquiry.

    I am sorry to confirm this kit has not yet been tested with bone samples, so we would not be certain how it would work. I would suggest that the protocol can be followed from the deproteinizing stage.

    Here...

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    I am planning to resect tumours from mice and then measure multiple metabolites from their tissue lysates. With reference to the ATP assay kit (ab83355), pyruvate assay kit (ab65342), L-lactate assay kit (ab65331) and citrate assay kit (ab83396), are all ...

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    Thank you for contacting us.

    The assay buffers in each of these kits is different. The buffers are optimized in order to get the best results. Please use the assay buffer for the sample, specific to each kit.

    I hope this information ...

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    What is the perchloric acid/KOH protocol (including reagents required) for deproteinization?

    Add 4 M perchloric acid to a final concentration of 1 M, vortex briefly and incubate on ice for at least 15 min. Centrifuge at 13,000xg for 2 min at 4oC and transfer supernatant to fresh tube. Excess perchloric acid is precipitated as KClO4 by adding an e...

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    can 540 nm be used for detection instead of 570 nm?

    Thank you for contacting us. I have heard back from the lab: If your instrument has a filter with a band width option of at least +/- 30 nm, then you will be able to use this filter to capture 99% of the signal. However, if this option is ...

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    Hi Tech,   I have a customer wanted to run her sample using ab83396. But her samples are supernatant microorganism. Do you think she can run and test it using this kit?   Thanks   Best regards,

    Thank you for contacting us and your interest in this product. If there is sufficient citrate in the cell lysates then the kit should be able to detect it. The kit can detect 0.1-10nmoles (~2 µM-10 mM). We recommend samples be prepared as follows:...

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    Dear Sir/Madam     Regarding Citrate Assay Kit (ab83396)   The customer would to know  full name of (WM) which is cap of Assay Buffer on protocol.   Best regarding

    Thank you for your enquiry. I can confirm that WM means wide mouth and refers to the cap code of the bottle. I hope this is helpful to you. If you have any further questions, please do not hesitate to contact us.

    

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"