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2 questions for ab119692
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Question 1
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Thursday 02-February-2012 |
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uses human skeletal muscle biopsies from children (i.e. has limited samples)
How much tissue do I need to startwith?
Is this different for each kit? |
ANSWER: |
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Thank you for contacting us.
I have heard back from the lab with the following information:
All these assays require different sample concentrations described in each booklet protocol - but in most cases is approximately 10ug/microplate well.
The good news is that normal whole muscle homogenate contains a good robust mitochondria signal so less is required, muscle samples are described in most if not all protocols.
Another good news is that the same preparation for any of the assays is suitable for all others. I would estimate that if a 1 mg of homogenate can be prepared (0.2mL at 5mg/mL).
This would provide enough material for each of the assays when diluted to the specific concentration in each assay.
We would recommend - if contemplating doing multiple assays - to include a mitochondrial sample as positive control such as ab110338 isolated mitochondria (note this is cow source)
I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information. |
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Question 2
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Thursday 26-January-2012 |
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Does this kit also work with mouse? |
ANSWER: |
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Thank you for your inquiry.
Unfortunately, this kit does not work in mouse. The capture antibody works in human but not in rat or mouse. Other species have not been tested yet.
This is the only Citrate Synthase Activity Assay Kit we carry. You may want to try http://www.biocompare.com to see if there is another commercially available.
I am sorry that this information could not be more helpful. Please contact us with any other questions. |
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