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Product name
Citrate Synthase Activity Assay Kit
Precision
| Sample | n | Mean | SD | CV% |
|---|---|---|---|---|
| 1 | 3 | 5.45% |
| Sample | n | Mean | SD | CV% |
|---|---|---|---|---|
| 1 | 3 | 8.3% |
Sample type
Cell culture extracts, Tissue Extracts
Assay type
Enzyme activity
Sensitivity
1.56 µg/ml
Range
1.56 µg/ml - 100 µg/ml
Cross reactivity
Reacts with
Human
Notes
Citrate synthase (CS, O75390) is the initial enzyme of the tricarboxylic acid (TCA) cycle. This enzyme is a 51.7 kDa enzyme that catalyzes the reaction of 2 carbon acetyl CoA with 4 carbon oxaloacetate to form the 6 carbon citrate (EC 2.3.3.1). This enzyme is an exclusive marker of the mitochondrial matrix. Citrate synthase is found in nearly all cells capable of oxidative metabolism.
Storage: All components are shipped cold. 10X Reaction Reagent mixture is shipped lyophilized. Before use, rehydrate the reaction mixture by adding 1mL base buffer. Vortex the tube thoroughly to dissolve the lyophilized powder to prepare 10X Reaction solution. After hydration unused amounts of the 10X Reaction solution should be stored at -80°C for 6 months. Store all other components at 4°C. This kit is stable for 6 months from receipt.
Storage: All components are shipped cold. 10X Reaction Reagent mixture is shipped lyophilized. Before use, rehydrate the reaction mixture by adding 1mL base buffer. Vortex the tube thoroughly to dissolve the lyophilized powder to prepare 10X Reaction solution. After hydration unused amounts of the 10X Reaction solution should be stored at -80°C for 6 months. Store all other components at 4°C. This kit is stable for 6 months from receipt.
Properties
Storage instructions
Please see Notes section
| Components | Identifier | 1 x 96 tests |
|---|---|---|
| 10X Blocking Buffer | P/N 8209802 | 1 x 6ml |
| 10X Reaction Reagent | P/N 8209524 | 1 vial |
| 20X Buffer | P/N 8203013 | 1 x 20ml |
| Base Buffer | P/N 8209733 | 1 x 25ml |
| CS Microplate | P/N 8209525 | 1 unit |
| Extraction Buffer | P/N 8201093 | 1 x 15ml |
Research areas
Pathway
Carbohydrate metabolism; tricarboxylic acid cycle; isocitrate from oxaloacetate: step 1/2.
Sequence similarities
Belongs to the citrate synthase family.
Cellular localization
Mitochondrion matrix.
Target information above from: UniProt accessionO75390
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Alternative names
- mitochondrial
- CISY_HUMAN
- Citrate synthase
- Cs
Database links
- Entrez Gene: 1431 Human
- SwissProt: O75390 Human
- Unigene: 430606 Human
Citrate Synthase Activity Assay Kit images:
ELISA - Citrate Synthase Activity Assay Kit (ab119692)
Figure 1. Example activity measurements from titrated HepG2 cells.
ELISA - Citrate Synthase Activity Assay Kit (ab119692)
Figure 2. Example activity measurements from titrated of Human heart homogenate.
Protocols
References for Citrate Synthase Activity Assay Kit (ab119692)
ab119692 has not yet been referenced specifically in any publications.
Publishing research using ab119692? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"