Anti-Citrate synthetase antibody (ab151607)
Key features and details
- Rabbit polyclonal to Citrate synthetase
- Suitable for: WB
- Reacts with: Rat, Human
- Isotype: IgG
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Overview
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Product name
Anti-Citrate synthetase antibody
See all Citrate synthetase primary antibodies -
Description
Rabbit polyclonal to Citrate synthetase -
Host species
Rabbit -
Tested applications
Suitable for: WBmore details -
Species reactivity
Reacts with: Rat, Human
Predicted to work with: Mouse, Horse, Chicken, Pig, Chimpanzee, Macaque monkey, Gorilla, Orangutan -
Immunogen
Synthetic peptide corresponding to Human Citrate synthetase aa 150-250 conjugated to keyhole limpet haemocyanin.
Database link: O75390 -
Positive control
- This antibody gave a positive signal in Rat Liver and Human Heart Mitochondrial tissue lysates as well as the following whole cell lysates: HepG2; Caco2; HEK293; HeLa; A375
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.40
Preservative: 0.02% Sodium azide
Constituent: PBS
Batches of this product that have a concentration < 1mg/ml may have BSA added as a stabilising agent. If you would like information about the formulation of a specific lot, please contact our scientific support team who will be happy to help. -
Concentration information loading...
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Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab151607 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB |
Use a concentration of 1 µg/ml. Detects a band of approximately 48 kDa (predicted molecular weight: 51 kDa).
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Notes |
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WB
Use a concentration of 1 µg/ml. Detects a band of approximately 48 kDa (predicted molecular weight: 51 kDa). |
Target
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Pathway
Carbohydrate metabolism; tricarboxylic acid cycle; isocitrate from oxaloacetate: step 1/2. -
Sequence similarities
Belongs to the citrate synthase family. -
Cellular localization
Mitochondrion matrix. - Information by UniProt
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Database links
- Entrez Gene: 100858903 Chicken
- Entrez Gene: 1431 Human
- Entrez Gene: 12974 Mouse
- Entrez Gene: 397519 Pig
- Entrez Gene: 170587 Rat
- Omim: 118950 Human
- SwissProt: P23007 Chicken
- SwissProt: O75390 Human
see all -
Alternative names
- CISY_HUMAN antibody
- Citrate synthase antibody
- Citrate synthase, mitochondrial antibody
see all
Images
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All lanes : Anti-Citrate synthetase antibody (ab151607) at 1 µg/ml
Lane 1 : Liver (Rat) Tissue Lysate
Lane 2 : Human Heart Mitochondrial Lysate
Lane 3 : HepG2 (Human hepatocellular liver carcinoma cell line) Whole Cell Lysate
Lane 4 : Caco 2 (Human colonic carcinoma cell line) Whole Cell Lysate
Lane 5 : HEK293 (Human embryonic kidney cell line) Whole Cell Lysate
Lane 6 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate
Lane 7 : A375 (Human melanoma cell line) Whole Cell Lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/10000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 51 kDa
Observed band size: 48 kDa why is the actual band size different from the predicted?
Additional bands at: 28 kDa (possible non-specific binding)
Exposure time: 30 secondsThe band observed at 48 kDa could potentially be a cleaved form of Citrate synthetase due to the presence of a 27 amino acid transit peptide.
This blot was produced using a 4-12% Bis-tris gel under the MOPS buffer system. The gel was run at 200V for 50 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 5% Bovine Serum Albumin before being incubated with ab151607 overnight at 4°C. Antibody binding was detected using an anti-rabbit antibody conjugated to HRP, and visualised using ECL development solution.
Datasheets and documents
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SDS download
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Datasheet download
References (0)
ab151607 has not yet been referenced specifically in any publications.