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- Secondary antibodies
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Product name
Anti-Claspin antibody
See all Claspin products (5) ...
Description
Rabbit polyclonal to Claspin
Tested applications
WB, IPmore details
Cross reactivity
Reacts with
Human
Immunogen
Synthetic peptide (Human) - which represents a portion of human Claspin encoded within exon 15.
Positive control
HeLa cell lysate.
Properties
Form
Liquid
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage buffer
Preservative: 0.1% Sodium Azide
Constituents: 8mM PBS, 60mM Citrate, 150mM Tris, pH 7-8
Concentration
Concentration information loading...
Purity
Immunogen affinity purified
Clonality
Polyclonal
Isotype
IgG
Research areas
Epigenetics and Nuclear Signaling >> Cell cycle >> Cell Cycle Inhibitors >> Other
Epigenetics and Nuclear Signaling >> DNA / RNA >> DNA Damage & Repair >> DNA Damage Response >> Other
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Western blot - Claspin antibody (ab3721)
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Applications
Show applications key
Our Abpromise guarantee covers the use of ab3721 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
WB: 1/1000 - 1/10000. Detects a band of approximately 180 kDa (predicted molecular weight: 150 kDa).
IP: Use a concentration of 1 - 5 µg/ml.
Target
Relevance
Claspin is required for checkpoint mediated cell cycle arrest in response to inhibition of DNA replication or to DNA damage induced by both ionizing and UV irradiation. It binds to BRCA1 and the checkpoint kinase CHEK1 and facilitates the ATR-dependent phosphorylation of both proteins. It can also bind to branched DNA structures and may associate with S-phase chromatin following formation of the pre-replication complex. This may indicate a role for this protein as a sensor which monitors the integrity of DNA replication forks.
Cellular localization
Nuclear
Alternative names
- claspin homolog (Xenopus laevis) antibody
- Claspin homolog antibody
- Clspn antibody
see all
Database links
- Entrez Gene: 63967 Human
- Omim: 605434 Human
- SwissProt: Q9HAW4 Human
Anti-Claspin antibody images:
Western blot - Claspin antibody (ab3721)
Predicted band size : 150 kDa
Sample: Hela cell lysate (20
Antibody: Affinity purified Rabbit anti-Claspin (ab3721) used at 1:1000 or 1:5000 dilution with (+) or without (-) blocking peptide.
Detection: ECL.
References for Anti-Claspin antibody (ab3721)
This product has been referenced in:
- Jiang K et al. Regulation of Chk1 includes chromatin association and 14-3-3 binding following phosphorylation on Ser-345. J Biol Chem 278:25207-17 (2003).Read more (PubMed: 12676962) »
See 1 publication for this product
Publishing research using ab3721? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"