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Overview

  • Product nameAnti-Claspin antibodySee all Claspin primary antibodies ...
  • Description
    Rabbit polyclonal to Claspin
  • Tested applicationsWB more details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat
  • Immunogen

    Recombinant fragment, corresponding to amino acids 1126-1330 of Human Claspin (NM_022111).

  • Positive control293T cell lysate

Properties

Applications

Our Abpromise guarantee covers the use of ab94945 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Notes
WB WB: 1/500 - 1/1000. Predicted molecular weight: 150 kDa.

Target

  • FunctionRequired for checkpoint mediated cell cycle arrest in response to inhibition of DNA replication or to DNA damage induced by both ionizing and UV irradiation. Adapter protein which binds to BRCA1 and the checkpoint kinase CHEK1 and facilitates the ATR-dependent phosphorylation of both proteins. Can also bind specifically to branched DNA structures and may associate with S-phase chromatin following formation of the pre-replication complex (pre-RC). This may indicate a role for this protein as a sensor which monitors the integrity of DNA replication forks.
  • Sequence similaritiesBelongs to the claspin family.
  • DomainThe C-terminus of the protein contains 3 potential CHEK1-binding motifs (CKB motifs). Potential phosphorylation sites within CKB motif 1 and CKB motif 2 are required for interaction with CHEK1.
  • Post-translational
    modifications    Phosphorylated. Undergoes ATR-dependent phosphorylation by CHEK1 during activation of DNA replication or damage checkpoints. Phosphorylation by CSNK1G1/CK1 promotes CHEK1 binding.
    Ubiquitinated by the anaphase promoting complex/cyclosome (APC/C) during G1 phase, leading to its degradation by the proteasome. Ubiquitination is mediated via its interaction with FZR1/CDH1. Following DNA damage, it is deubiquitinated by USP28 in G2 phase, preventing its degradation.
  • Cellular localizationNucleus.

    • Target information above from: UniProt accession Q9HAW4 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Database links
    • Alternative names
        Claspin antibodyclaspin homolog (Xenopus laevis) antibodyClaspin homolog antibody
        Clspn antibodyCLSPN protein antibodyCLSPN_HUMAN antibodyhClaspin antibodyHu Claspin antibody
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    Anti-Claspin antibody images

    • Western blot - Claspin antibody (ab94945)
      Western blot - Claspin antibody (ab94945)
      Anti-Claspin antibody (ab94945) at 1/500 dilution + 293T cell lysate

      Predicted band size : 150 kDa

    References for Anti-Claspin antibody (ab94945)

    ab94945 has not yet been referenced specifically in any publications.

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