Anti-Clathrin antibody [X22] - Membrane Vesicle Marker (ab2731)

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17 Specific references

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Publishing research using ab2731? Please let us know so that we can cite the reference in this datasheet

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ab2731 has been referenced in 17 publications.

Butler CA et al. Glucocorticoid receptor ß and histone deacetylase 1 and 2 expression in the airways of severe asthma. Thorax 67:392-8 (2012). WB; Human. PubMed: 22156779
Shieh JC et al. Endocytosis regulates cell soma translocation and the distribution of adhesion proteins in migrating neurons. PLoS One 6:e17802 (2011). ICC/IF; Mouse. PubMed: 21445347
Kuo YC & Chen HH Effect of electromagnetic field on endocytosis of cationic solid lipid nanoparticles by human brain-microvascular endothelial cells. J Drug Target 18:447-56 (2010). ICC/IF; Human. PubMed: 20528098
Teckchandani A et al. Quantitative proteomics identifies a Dab2/integrin module regulating cell migration. J Cell Biol 186:99-111 (2009). ICC/IF; Human. PubMed: 19581412
Yu C et al. Myosin VI undergoes cargo-mediated dimerization. Cell 138:537-48 (2009). ICC/IF; Human. PubMed: 19665975
Wälchli S et al. Characterization of clathrin and Syk interaction upon Shiga toxin binding. Cell Signal 21:1161-8 (2009). WB, IP; Human. PubMed: 19289168
Puri C Loss of myosin VI no insert isoform (NoI) induces a defect in clathrin-mediated endocytosis and leads to caveolar endocytosis of transferrin receptor. J Biol Chem 284:34998-5014 (2009). ICC/IF; Human. PubMed: 19840950
Ezratty EJ et al. Clathrin mediates integrin endocytosis for focal adhesion disassembly in migrating cells. J Cell Biol 187:733-47 (2009). ICC/IF; Mouse. PubMed: 19951918
Markovic D et al. Intracellular mechanisms regulating corticotropin-releasing hormone receptor-2beta endocytosis and interaction with extracellularly regulated kinase 1/2 and p38 mitogen-activated protein kinase signaling cascades. Mol Endocrinol 22:689-706 (2008). PubMed: 18048641
Grøvdal LM et al. Dysregulation of Ack1 inhibits down-regulation of the EGF receptor. Exp Cell Res 314:1292-300 (2008). PubMed: 18262180
Pryor PR et al. Molecular basis for the sorting of the SNARE VAMP7 into endocytic clathrin-coated vesicles by the ArfGAP Hrb. Cell 134:817-27 (2008). ICC/IF; Rat. PubMed: 18775314
Cortese K et al. Clathrin and LRP-1-independent constitutive endocytosis and recycling of uPAR. PLoS ONE 3:e3730 (2008). ICC/IF; Human. PubMed: 19008962
Spudich G et al. Myosin VI targeting to clathrin-coated structures and dimerization is mediated by binding to Disabled-2 and PtdIns(4,5)P2. Nat Cell Biol 9:176-83 (2007). ICC/IF; Human. PubMed: 17187061
Samaniego R et al. Rho/ROCK and myosin II control the polarized distribution of endocytic clathrin structures at the uropod of moving T lymphocytes. J Cell Sci 120:3534-43 (2007). PubMed: 17895369
Tateno H et al. Distinct endocytic mechanisms of CD22 (Siglec-2) and Siglec-F reflect roles in cell signaling and innate immunity. Mol Cell Biol 27:5699-710 (2007). ICC/IF; Mouse. PubMed: 17562860
Bowers K et al. Degradation of Endocytosed Epidermal Growth Factor and Virally Ubiquitinated Major Histocompatibility Complex Class I Is Independent of Mammalian ESCRTII. J Biol Chem 281:5094-105 (2006). PubMed: 16371348
Sun L et al. Transmitter release face Ca2+ channel clusters persist at isolated presynaptic terminals. Eur J Neurosci 23:1391-6 (2006). PubMed: 16553800

Publishing research using ab2731? Please let us know so that we can cite the reference in this datasheet

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

Immunocytochemistry/ Immunofluorescence - Clathrin antibody [X22] - Membrane Vesicle Marker (ab2731)

ab2731 staining human HEK 293 cells by ICC/IF. Cells were PFA fixed and permeabilized in 0.01% Triton X-100 in PBS prior to blocking in 1% BSA for 1 hour at 22°C. The primary antibody was diluted 1/75 and incubated with the sample for 1 hour at 22°C. An Alexa Fluor® 405 conjugated goat anti-mouse antibody diluted 1/400 was used as the secondary.

This image is courtesy of an Abreview submitted by Mrs Danijela Markovic

Western blot - Clathrin antibody [X22] - Membrane Vesicle Marker (ab2731)

All lanes : Anti-Clathrin antibody [X22] - Membrane Vesicle Marker (ab2731) at 1/500 dilution

Lane 1 : HepG2 (Human hepatocellular liver carcinoma cell line) Whole Cell Lysate
Lane 2 : Raji (Human Burkitt's lymphoma cell line) Whole Cell Lysate

Lysates/proteins at 10 µg per lane.

Secondary
Goat polyclonal to Mouse IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution

Observed band size : 180 kDa (why is the actual band size different from the predicted?)
Additional bands at : 240 kDa,450 kDa. We are unsure as to the identity of these extra bands.

Immunocytochemistry/ Immunofluorescence - Clathrin antibody [X22] - Membrane Vesicle Marker (ab2731)

ab2731 staining Clathrin - Membrane Vesicle Marker in Rat hypothalamus primary cells by ICC/IF (immunocytochemistry/immunofluorescence). Cells were fixed with paraformaldehyde, permeabilized with 0.5% TX-100 and blocked with 5% serum for 20 minutes at 25ºC. Samples were incubated with primary antibody (1/500 in 1% goat serum; 0.1% TX100; PBS) for 16 hours at 4ºC. An Alexa Fluor^®546-conjugated Goat polyclonal to mouse IgG, dilution 1/500, was used as secondary antibody.

This image is courtesy of an Abreview submitted by Dr Vladimir Milenkovic

Immunocytochemistry/ Immunofluorescence - Clathrin antibody [X22] - Membrane Vesicle Marker (ab2731)

ab2731 staining Clathrin in pig retinal pigment epithelium (RPE) cells by Immunocytochemistry/ Immunofluorescence. Cells were fixed with paraformaldehyde, permeabilized with 0.5% Triton ×100 and blocked with 5% serum for 20 minutes at 25⁰C. Samples were incubated with primary antibody (1/500: in 0.1% TX100, 1% goat serum, 1X PBS) for 16 hours at 4°C. An Alexa Fluor^®546-conjugated goat polyclonal to mouse IgG was used as secondary antibody at 1/500 dilution.

This image is a courtesy of Vladimir Milenkovic

Flow Cytometry - Anti-Clathrin antibody [X22] - Membrane Vesicle Marker (ab2731)

ab2731 staining clathrin in Human platelets by Flow Cytometry. Platelets were isolated form Platelet rich plasma and suspended in PBS, fixed with paraformaldehyde and permeabilized with 0.1% Triton-X100 in 2% BSA for 30 minutes. The sample was incubated with the primary antibody (1/200 in PBS + 2% BSA) for 18 hours at 4ºC. An Alexa Fluor^®594-conjugated Goat anti-mouse IgG polyclonal (1/500) was used as the secondary antibody.
Gating Strategy: Platelets

This image is courtesy of an Abreview submitted by Mahesh Shivananjappa