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Immunohistochemistry (Paraffin-embedded sections) - Clathrin antibody (ab59710)
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Immunohistochemistry (Paraffin-embedded sections) - Clathrin antibody (ab59710)
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Product Name
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Clathrin antibody
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See all Clathrin - Membrane Vesicle Marker antibodies (2)...
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Product type
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Primary antibodies
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Description
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Rabbit polyclonal to Clathrin
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Immunogen
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Rat/Human Peptide sequence:kagllqralehftdlydikr, corresponding to amino acids 619-638 of Rat Clathrin heavy chain, conjugated to diphtheria toxin.
BLAST 'kagllqralehftdlydikr' with  or 
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Reacts with
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Rat
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Predicted to react
(species key)
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Hu, Ms, Many, Ce, Chk, Cow, Dm, Xl, Zfsh
(Due to sequence homology)
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Tested applications
(see key)
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ICC/IF, IHC-Fr, IHC-P, WB
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Abreviews
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Application notes
(see key)
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Recommended dilutions
ICC/IF: 1/100 - 1/400.
IHC-Fr: 1/1000 - 1/4000.
IHC-P: 1/1000 - 1/4000.
WB: 1/1000 - 1/8000. Predicted molecular weight: 192 kDa.
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
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Positive control
(see definition)
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Rat lung tissue.
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Cellular localization
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Cytoplasmic face of coated pits and vesicles.
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Research areas
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Tags & Cell Markers >> Subcellular Markers >> Organelles >> Caveolae and Clathrin
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Relevance
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Membrane vesicle formation is a process required for the endocytosis and biosynthesis of various secreted and membrane bound proteins. Clathrin is a protein which assembles into a polyhedral network on the cell membrane as the membrane invaginates, forming a coated pit which is essential to endocytosis. Clathrin is composed of three polypeptides, a 180 kDa heavy chain and two 32-38 kDa light chains which combine to create a distinct three-legged triskelion. It is this morphology which allows Clathrin to form its unique polyhedral network.
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Database links
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The links below go to external sites and will open in a new browser window |
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Raised in
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Rabbit
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Clonality
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Polyclonal
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Isotype
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IgG
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Purity
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Whole antiserum
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Storage buffer
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Preservative: 0.02% Thimerosal (merthiolate)
Constituents: Whole serum Material safety datasheet (MSDS) for this product:
Thimerosal MSDS
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Form
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Liquid
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Concentration
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Concentration not determined - why is this?
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Storage instructions
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Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
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At Abcam, we have one centralized database to hold all of our product information, so that everything we know about this Clathrin antibody is on this datasheet. But please do contact us if you would like any reassurance! |
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See below for Clathrin antibody images, references, products related to ab59710 and other tools.
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Clathrin antibody images:
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 Immunohistochemistry (Paraffin-embedded sections) - Clathrin antibody (ab59710)
ab59710 at 1/1000, overnight, secondary antibody biotinylated goat anti-sheep at 1/3000. Figure A is a low power image showing intense staining of non-ciliated bronchiolar cells (Clara cells) and lesser staining in alveolar type II cells. Figure B is a higher power image of the staining of the non ciliated bronchiolar cells.
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Immunohistochemistry (Paraffin-embedded sections) - Clathrin antibody (ab59710)
ab59710 at 1/100, overnight, secondary antibody donkey anti-rabbit CY2 ® at 1/50. Confocal image shows intense staining of non-ciliated bronchiolar cells (Clara cells).
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Search PubMed (MEDLINE) for references to Clathrin
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Clathrin antibody - more information
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Customer reviews (feedback) regarding Clathrin antibody |
Customer FAQs regarding Clathrin antibody |
Protocols for Clathrin antibody |
Price and availability of products related to Clathrin antibody |
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Clathrin antibody - related products:
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Clathrin antibody - other tools:
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Contact Abcam with a Technical Enquiry about Clathrin antibody |
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All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
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