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Products:Tags & Cell Markers >> Subcellular Markers >> Organelles >> Caveolae and Clathrin
Overview
Product name
Anti-Clathrin antibody
See all Clathrin products (2) ...
Description
Rabbit polyclonal to Clathrin
Tested applications
ICC/IF, WB, IHC-Fr, IHC-P, IPmore details
Cross reactivity
Reacts with
Rat, Human
Predicted to work with
Mouse, Chicken, Cow, Xenopus laevis, Caenorhabditis elegans, Fruit fly (Drosophila melanogaster), Zebrafish, a wide range of other species
Immunogen
Rat/Human Peptide sequence:kagllqralehftdlydikr, corresponding to amino acids 619-638 of Rat Clathrin heavy chain, conjugated to diphtheria toxin.
kagllqrale hftdlydikr
Positive control
Rat lung tissue.
Properties
Form
Liquid
Storage instructions
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Storage buffer
Preservative: 0.02% Thimerosal (merthiolate)
Constituents: Whole serum
Purity
Whole antiserum
Clonality
Polyclonal
Isotype
IgG
Research areas
Tags & Cell Markers >> Subcellular Markers >> Organelles >> Caveolae and Clathrin
Applications
Show applications key
Our Abpromise guarantee covers the use of ab59710 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
ICC/IF
ICC/IF: 1/100 - 1/400.ICC/IF: 1/100 - 1/400.
WB: 1/1000 - 1/8000.Predicted molecular weight: 192 kDa.
IHC-Fr
IHC-Fr: 1/1000 - 1/4000.IHC-Fr: 1/1000 - 1/4000.
IHC-P: 1/1000 - 1/4000.
IP: Use at an assay dependent dilution.
Target
Relevance
Membrane vesicle formation is a process required for the endocytosis and biosynthesis of various secreted and membrane bound proteins. Clathrin is a protein which assembles into a polyhedral network on the cell membrane as the membrane invaginates, forming a coated pit which is essential to endocytosis. Clathrin is composed of three polypeptides, a 180 kDa heavy chain and two 32-38 kDa light chains which combine to create a distinct three-legged triskelion. It is this morphology which allows Clathrin to form its unique polyhedral network.
Cellular localization
Cytoplasmic face of coated pits and vesicles.
Alternative names
- Adapter related protein complex 1 beta 1 subunit antibody
- Adaptor Protein Complex AP1 Beta 1 Subunit antibody
- ADTB1 antibody
see all
Database links
- Entrez Gene: 162 Human
- Entrez Gene: 11764 Mouse
- Entrez Gene: 29663 Rat
- Omim: 600157 Human
- SwissProt: P49951 Cow
- SwissProt: P29742 Fruit fly (Drosophila melanogaster)
- SwissProt: Q00610 Human
- SwissProt: O35643 Mouse
see all
Anti-Clathrin antibody images:
Immunohistochemistry (Paraffin-embedded sections) - Clathrin antibody (ab59710)
ab59710 at 1/1000, overnight, secondary antibody biotinylated goat anti-sheep at 1/3000.
Figure A is a low power image showing intense staining of non-ciliated bronchiolar cells (Clara cells) and lesser staining in alveolar type II cells.
Figure B is a higher power image of the staining of the non ciliated bronchiolar cells.
Immunohistochemistry (Paraffin-embedded sections) - Clathrin antibody (ab59710)
ab59710 at 1/100, overnight, secondary antibody donkey anti-rabbit CY2 ® at 1/50. Confocal image shows intense staining of non-ciliated bronchiolar cells (Clara cells).
References for Anti-Clathrin antibody (ab59710)
ab59710 has not yet been referenced specifically in any publications.
Publishing research using ab59710? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"