Anti-Clathrin light chain antibody (ab103553)
Key features and details
- Rabbit polyclonal to Clathrin light chain
- Suitable for: WB
- Reacts with: Mouse, Human
- Isotype: IgG
Overview
-
Product name
Anti-Clathrin light chain antibody
See all Clathrin light chain primary antibodies -
Description
Rabbit polyclonal to Clathrin light chain -
Host species
Rabbit -
Tested applications
Suitable for: WBmore details -
Species reactivity
Reacts with: Mouse, Human -
Immunogen
Recombinant full length protein within Human Clathrin light chain aa 1-250. The exact immunogen sequence used to generate this antibody is proprietary information. If additional detail on the immunogen is needed to determine the suitability of the antibody for your needs, please contact our Scientific Support team to discuss your requirements.
-
Positive control
- Human stomach and Mouse spleen tissue lysates, Clathrin light chain transfected 293T cell line lysate.
-
General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
-
Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.40
Constituent: 100% PBS -
Concentration information loading...
-
Purity
Protein A purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
-
Compatible Secondaries
-
Isotype control
-
Positive Controls
-
Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab103553 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
---|---|---|
WB |
1/500 - 1/1000. Predicted molecular weight: 23 kDa.
|
Notes |
---|
WB
1/500 - 1/1000. Predicted molecular weight: 23 kDa. |
Target
-
Function
Clathrin is the major protein of the polyhedral coat of coated pits and vesicles. -
Cellular localization
Cytoplasmic vesicle membrane. Membrane > coated pit. Cytoplasmic face of coated pits and vesicles. - Information by UniProt
-
Database links
- Entrez Gene: 1211 Human
- Entrez Gene: 1212 Human
- Entrez Gene: 12757 Mouse
- Entrez Gene: 74325 Mouse
- Omim: 118960 Human
- Omim: 118970 Human
- SwissProt: P09496 Human
- SwissProt: P09497 Human
see all -
Alternative names
- Clathrin light chain A antibody
- Clathrin light chain B antibody
- Clathrin light chain LCA antibody
see all
Images
-
Anti-Clathrin light chain antibody (ab103553) at 1/500 dilution + Human stomach tissue lysate at 25 µg
Predicted band size: 23 kDa -
Anti-Clathrin light chain antibody (ab103553) at 1/500 dilution + Mouse spleen tissue lysate at 25 µg
Predicted band size: 23 kDa -
All lanes : Anti-Clathrin light chain antibody (ab103553) at 1/500 dilution
Lane 1 : Clathrin light chain transfected 293T cell line lysate
Lane 2 : Non-transfected 293T cell line lysate
Lysates/proteins at 25 µg per lane.
Predicted band size: 23 kDa
Datasheets and documents
-
Datasheet download
References (1)
ab103553 has been referenced in 1 publication.
- Martins-de-Souza D et al. The protein interactome of collapsin response mediator protein-2 (CRMP2/DPYSL2) reveals novel partner proteins in brain tissue. Proteomics Clin Appl 9:817-31 (2015). PubMed: 25921334