Anti-Clathrin light chain antibody (ab129326)
Key features and details
- Goat polyclonal to Clathrin light chain
- Suitable for: WB, IHC-P
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-Clathrin light chain antibody
See all Clathrin light chain primary antibodies -
Description
Goat polyclonal to Clathrin light chain -
Host species
Goat -
Specificity
ab129326 is expected to recognize all reported isoforms of light chains a and b. -
Tested applications
Suitable for: WB, IHC-Pmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Mouse, Rat, Horse, Chicken, Cow, Dog, Pig, Xenopus laevis, Zebrafish, Gorilla -
Immunogen
Synthetic peptide:
CDFNPKSSKQAKD
, corresponding to internal sequence amino acids 218-230 of Human Clathrin light chain a (NP_009027.1). -
Positive control
- Human Spleen tissue; Human Frontal Cortex lysate.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
Storage buffer
pH: 7.30
Preservative: 0.02% Sodium azide
Constituents: 99% Tris buffered saline, 0.5% BSA -
Concentration information loading...
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Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab129326 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
---|---|---|
WB |
Use a concentration of 0.1 - 0.3 µg/ml. Detects a band of approximately 23, 35 kDa (predicted molecular weight: 23 kDa).
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IHC-P |
Use a concentration of 2.5 - 3.75 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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Notes |
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WB
Use a concentration of 0.1 - 0.3 µg/ml. Detects a band of approximately 23, 35 kDa (predicted molecular weight: 23 kDa). |
IHC-P
Use a concentration of 2.5 - 3.75 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
Target
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Function
Clathrin is the major protein of the polyhedral coat of coated pits and vesicles. -
Cellular localization
Cytoplasmic vesicle membrane. Membrane > coated pit. Cytoplasmic face of coated pits and vesicles. - Information by UniProt
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Database links
- Entrez Gene: 416226 Chicken
- Entrez Gene: 427284 Chicken
- Entrez Gene: 281078 Cow
- Entrez Gene: 281079 Cow
- Entrez Gene: 281698 Cow
- Entrez Gene: 474765 Dog
- Entrez Gene: 489104 Dog
- Entrez Gene: 100055375 Horse
see all -
Alternative names
- Clathrin light chain A antibody
- Clathrin light chain B antibody
- Clathrin light chain LCA antibody
see all
Images
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ab129326, at 2.5 µg/ml, staining Clathrin light chain in formalin-fixed, paraffin-embedded Human spleen tissue by Immunohistochemistry.
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Anti-Clathrin light chain antibody (ab129326) at 0.1 µg/ml + Human Frontal Cortex lysate in RIPA buffer at 35 µg
Developed using the ECL technique.
Predicted band size: 23 kDa
Primary incubation was 1 hour.
Protocols
Datasheets and documents
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Datasheet download
References (0)
ab129326 has not yet been referenced specifically in any publications.