Anti-Clathrin light chain antibody (ab77542)
Key features and details
- Goat polyclonal to Clathrin light chain
- Suitable for: WB
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-Clathrin light chain antibody
See all Clathrin light chain primary antibodies -
Description
Goat polyclonal to Clathrin light chain -
Host species
Goat -
Specificity
Expected to recognize all reported isoforms of light chains a and b. -
Tested applications
Suitable for: WBmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Mouse, Rat, Horse, Chicken, Cow, Dog, Pig, Xenopus laevis, Chimpanzee, Zebrafish, Macaque monkey -
Immunogen
Synthetic peptide:
CDFNPKSSKQAKD
from Human Clathrin light chain(NP_001824.1; NP_009027.1; NP_001070145.1; NP_001825.1; NP_009028.1) -
Positive control
- Recombinant Human Clathrin light chain protein (ab126693) can be used as a positive control in WB. Human frontal cortex lysate
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles. -
Storage buffer
pH: 7.30
Preservative: 0.02% Sodium azide
Constituents: 0.5% BSA, Tris buffered saline -
Concentration information loading...
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Purity
Immunogen affinity purified -
Purification notes
Purified from goat serum by ammonium sulphate precipitation, followed by antigen affinity chromatography using the immunizing peptide. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab77542 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
---|---|---|
WB |
Notes |
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WB: Use at a concentration of 0.1 - 0.3 µg/ml. Predicted molecular weight: 23 kDa.
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
Target
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Function
Clathrin is the major protein of the polyhedral coat of coated pits and vesicles. -
Cellular localization
Cytoplasmic vesicle membrane. Membrane > coated pit. Cytoplasmic face of coated pits and vesicles. - Information by UniProt
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Database links
- Entrez Gene: 416226 Chicken
- Entrez Gene: 427284 Chicken
- Entrez Gene: 281078 Cow
- Entrez Gene: 281079 Cow
- Entrez Gene: 281698 Cow
- Entrez Gene: 474765 Dog
- Entrez Gene: 489104 Dog
- Entrez Gene: 100055375 Horse
see all -
Alternative names
- Clathrin light chain A antibody
- Clathrin light chain B antibody
- Clathrin light chain LCA antibody
see all
Images
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Anti-Clathrin light chain antibody (ab77542) at 0.1 µg/ml + Human frontal cortex lysate in RIPA buffer at 35 µg
Predicted band size: 23 kDa
Observed band size: 23 kDa
Additional bands at: 35 kDa (possible isoform)
The observed molecular weight of ~35kDa corresponds to earlier findings in literature with different antibodies (Okamoto et al, Am J Physiol Cell Physiol. 2000 Sep;279(3):C833-51.; PMID: 10942733)
Datasheets and documents
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Datasheet download
References (1)
ab77542 has been referenced in 1 publication.
- Liu H et al. Clathrin-mediated endocytosis in living host cells visualized through quantum dot labeling of infectious hematopoietic necrosis virus. J Virol 85:6252-62 (2011). WB . PubMed: 21525360