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Products:Signal Transduction >> Cytoskeleton / ECM >> Cell Adhesion >> Tight Junctions
Overview
Product name
Anti-Claudin 17 antibody - BSA and Azide free
Description
Rabbit polyclonal to Claudin 17 - BSA and Azide free
Tested applications
WB, IHC-Pmore details
Cross reactivity
Reacts with
Human
Predicted to work with
Mouse, Rat, Dog, Pig
Immunogen
A region within synthetic peptide: GSNERAKAYL LGTSGVLFIL TGIFVLIPVS WTANIIIRDF YNPAIHIGQK, corresponding to internal sequence amino acids 109-158 of Human Claudin 17
GSNERAKAYL LGTSGVLFIL TGIFVLIPVS WTANIIIRDF YNPAIHIGQK
Positive control
Jurkat cell lysate for western blot and human lung and spleen for immunohistochemistry.
Properties
Form
Liquid
Storage instructions
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze / thaw cycles.
Storage buffer
Preservative: None.
Constituents: 2% Sucrose, PBS.
Concentration
Concentration information loading...
Purity
Protein A purified
Purification notes
This antibody was purified by chromatography.
Clonality
Polyclonal
Isotype
IgG
Research areas
Signal Transduction >> Cytoskeleton / ECM >> Cell Adhesion >> Tight Junctions
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Western blot - Claudin 17 antibody - BSA and Azide free (ab23333)
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Claudin 17 antibody - BSA and Azide free (ab23333)
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Claudin 17 antibody - BSA and Azide free (ab23333)
(enlarge)
Applications
Show applications key
Our Abpromise guarantee covers the use of ab23333 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
WB: Use a concentration of 2 µg/mlDetects a band of approximately 30 kDa (predicted molecular weight: 25 kDa).
IHC-P: Use a concentration of 4 - 8 µg/ml.
Target
Relevance
Claudin proteins are a family of transmembrane proteins associated with tight junctions, and different tissues exhibit different Claudin composition. There are 18 homolgs present in humans, and mutations in any of these proteins cause a variety of human disease, this indicates their physiological importance. CLDN17, clustered with CLDN8 at human chromosome 21q22.11, is a four transmembrane protein with WWCC motif, defined by W-X(17-22)-W-X(2)-C-X(8-10)-C.
Cellular localization
Integral membrane protein. Tight junctions.
Alternative names
- CLDN17 antibody
Database links
- Entrez Gene: 26285 Human
- Entrez Gene: 239931 Mouse
- Omim: 609131 Human
- SwissProt: P56750 Human
- SwissProt: Q8BXA6 Mouse
- Unigene: 258589 Human
- Unigene: 126860 Mouse
Anti-Claudin 17 antibody - BSA and Azide free images:
Western blot - Claudin 17 antibody - BSA and Azide free (ab23333)
Anti-Claudin 17 antibody - BSA and Azide free (ab23333) at 2 µg/ml + Jurkat cell lysate (10ug)
Secondary
HRP conjugated anti-Rabbit IgG at 1/50,000 - 1/100,000 dilution.
Predicted band size : 25 kDa
Observed band size : 30 kDa (why is the actual band size different from the predicted?)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Claudin 17 antibody - BSA and Azide free (ab23333)
Paraffin-embedded sections of Human spleen cells incubated with ab23333. The antibody was used at a range of 4.0-8.0µg/ml. Cells stained positive for Cluadin 17 are indicated by arrows. 400x magnification.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Claudin 17 antibody - BSA and Azide free (ab23333)
Paraffin-embedded sections of Human alveolar cells incubated with ab23333. The antibody was used at a range of 4.0-8.0µg/ml. Cells stained positive for Claudin 17 are indicated by arrows. 400x magnification.
References for Anti-Claudin 17 antibody - BSA and Azide free (ab23333)
This product has been referenced in:
- Farwick Met al. Fifty-kDa hyaluronic acid upregulates some epidermal genes without changing TNF-a expression in reconstituted epidermis. Skin Pharmacol Physiol 24:210-7 (2011). WB; Human.Read more (PubMed: 21412035) »
- Katoh M & Katoh M Identification and characterization of human PPFIA4 gene in silico. Int J Mol Med 12:1009-14 (2003).Read more (PubMed: 14612982) »
See all 2 publications for this product
Publishing research using ab23333? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"