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Products:Microbiology >> Interspecies Interaction >> Host Virus Interaction
Overview
Product name
Anti-Claudin 6 antibody
See all Claudin 6 products (5) ...
Description
Rabbit polyclonal to Claudin 6
Tested applications
WB, ELISA, IHC-P, ICC/IFmore details
Cross reactivity
Reacts with
Human
Immunogen
Synthetic peptide derived from an internal sequence of human Claudin 6.
Positive control
human ovarian tissue, HUVEC cells, extracts from Jurkat cells.
Properties
Form
Liquid
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage buffer
Preservative: 0.02% Sodium Azide
Constituents: 50% Glycerol, PBS, 150mM Sodium chloride, pH 7.4
Concentration
Concentration information loading...
Purity
Immunogen affinity purified
Clonality
Polyclonal
Isotype
IgG
Applications
Show applications key
Our Abpromise guarantee covers the use of ab75055 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
WB: 1/500 - 1/1000.Detects a band of approximately 25 kDa (predicted molecular weight: 23 kDa).
ELISA
ELISA: 1/40000ELISA: 1/40000
IHC-P: 1/50 - 1/100.
ICC/IF: 1/500 - 1/1000.
Target
Function
Plays a major role in tight junction-specific obliteration of the intercellular space.
Sequence similarities
Belongs to the claudin family.
Cellular localization
Cell junction > tight junction. Cell membrane.
Target information above from: UniProt accessionP56747
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Alternative names
- Claudin-6 antibody
- Claudin6 antibody
- CLD6_HUMAN antibody
see all
Database links
- Entrez Gene: 9074 Human
- SwissProt: P56747 Human
- Unigene: 533779 Human
Anti-Claudin 6 antibody images:
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Claudin 6 antibody (ab75055)
Human ovarian tissue with ab75055 at 1/50 dilution, in the absence and presence of the immunising peptide.
Immunocytochemistry/ Immunofluorescence - Claudin 6 antibody (ab75055)
Huvec cells with ab75055 at 1/500 dilution, in the absence and presence of the immunising peptide.
Western blot - Claudin 6 antibody (ab75055)
All lanes : Anti-Claudin 6 antibody (ab75055) at 1/500 dilution
Lane 1 : extracts from Jurkat cells
Lane 2 : extracts from Jurkat cells with immunising peptide at 5 µg
Lysates/proteins at 5 µg per lane.
Predicted band size : 23 kDa
Observed band size : 25 kDa (why is the actual band size different from the predicted?)
Additional bands at : <19 kDa. We are unsure as to the identity of these extra bands.
References for Anti-Claudin 6 antibody (ab75055)
ab75055 has not yet been referenced specifically in any publications.
Publishing research using ab75055? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"