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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Claudin 6 antibody (ab75055)
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Immunocytochemistry/ Immunofluorescence - Claudin 6 antibody (ab75055)
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Western blot - Claudin 6 antibody (ab75055)
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Product Name
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Claudin 6 antibody
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See all Claudin 6 antibodies (2)...
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Product type
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Primary antibodies
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Description
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Rabbit polyclonal to Claudin 6
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Immunogen
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Synthetic peptide derived from an internal sequence of human Claudin 6.
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Reacts with
(species key)
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Hu
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Tested applications
(see key)
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ELISA, ICC/IF, IHC-P, WB
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Abreviews
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Application notes
(see key)
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Recommended dilutions
ELISA: 1/40000.
ICC/IF: 1/500 - 1/1000.
IHC-P: 1/50 - 1/100.
WB: 1/500 - 1/1000. Detects a band of approximately 25 kDa (predicted molecular weight: 23 kDa).
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
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Positive control
(see definition)
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human ovarian tissue, HUVEC cells, extracts from Jurkat cells.
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Cellular localization
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Cell Membrane
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Research areas
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Developmental Biology >> Lineage specification >> Endoderm
Signal Transduction >> Cytoskeleton / ECM >> Cell Adhesion >> Tight Junctions
Stem Cells >> Lineage Markers >> Endoderm
Microbiology >> Interspecies Interaction >> Host Virus Interaction
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Relevance
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Claudin 6 is a multipass transmembrane protein in the Claudin family. Claudin 6 is expressed by epithelial cells where it participates in tissue development and the maintenance of tight junction integrity. Claudin 6 is also a global marker of definitive endoderm. Directly interacts with TJP1/ZO-1, TJP2/ZO-2 and TJP3/ZO-3.
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Database links
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The links below go to external sites and will open in a new browser window |
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Raised in
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Rabbit
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Clonality
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Polyclonal
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Isotype
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IgG
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Purity
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Immunogen affinity purified
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Storage buffer
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Preservative: 0.02% Sodium Azide
Constituents: 50% Glycerol, PBS, 150mM Sodium chloride, pH 7.4 Material safety datasheets (MSDS) for this product:
Glycerol MSDS
Sodium Azide MSDS
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Form
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Liquid
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Concentration
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1.000 mg/ml
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Storage instructions
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Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
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At Abcam, we have one centralized database to hold all of our product information, so that everything we know about this Claudin 6 antibody is on this datasheet. But please do contact us if you would like any reassurance! |
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See below for Claudin 6 antibody images, references, products related to ab75055 and other tools.
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Claudin 6 antibody images:
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 Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Claudin 6 antibody (ab75055)
Human ovarian tissue with ab75055 at 1/50 dilution, in the absence and presence of the immunising peptide.
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Immunocytochemistry/ Immunofluorescence - Claudin 6 antibody (ab75055)
Huvec cells with ab75055 at 1/500 dilution, in the absence and presence of the immunising peptide.
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Western blot - Claudin 6 antibody (ab75055)
All lanes : Claudin 6 antibody (ab75055) at 1/500 dilution
Lane 1 : extracts from Jurkat cells
Lane 2 : extracts from Jurkat cells with immunising peptide at 5 µg
Lysates/proteins at 5 µg per lane.
Predicted band size : 23 kDa
Observed band size : 25 kDa (why is the actual band size different from the predicted?)
Additional bands at : <19 kDa. We are unsure as to the identity of these extra bands.
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Search PubMed (MEDLINE) for references to Claudin 6
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Claudin 6 antibody - more information
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Customer reviews (feedback) regarding Claudin 6 antibody |
Customer FAQs regarding Claudin 6 antibody |
Protocols for Claudin 6 antibody |
Price and availability of products related to Claudin 6 antibody |
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Claudin 6 antibody - related products:
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Claudin 6 antibody - other tools:
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Contact Abcam with a Technical Enquiry about Claudin 6 antibody |
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All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
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