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Western blot - Claudin 6 (phospho Y219) antibody (ab78347)
(enlarge)
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Product Name
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Claudin 6 (phospho Y219) antibody
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See all Claudin 6 antibodies (2)...
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Product type
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Primary antibodies
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Description
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Rabbit polyclonal to Claudin 6 (phospho Y219)
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Immunogen
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Synthesized phosphopeptide derived from human Claudin 6 around the phosphorylation site of tyrosine 219.
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Reacts with
(species key)
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Hu, Ms
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Specificity
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This antibody detects endogenous levels of Claudin 6 only when phosphorylated at tyrosine 219.
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Tested applications
(see key)
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ELISA, WB
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Abreviews
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Application notes
(see key)
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Recommended dilutions
ELISA: 1/5000.
WB: 1/500 - 1/1000. Detects a band of approximately 23 kDa (predicted molecular weight: 23 kDa).
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
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Positive control
(see definition)
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Extracts from RAW264.7 cells.
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Cellular localization
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Cell Membrane
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Research areas
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Developmental Biology >> Lineage specification >> Endoderm
Signal Transduction >> Cytoskeleton / ECM >> Cell Adhesion >> Tight Junctions
Stem Cells >> Lineage Markers >> Endoderm
Microbiology >> Interspecies Interaction >> Host Virus Interaction
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Relevance
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Claudin 6 is a multipass transmembrane protein in the Claudin family. Claudin 6 is expressed by epithelial cells where it participates in tissue development and the maintenance of tight junction integrity. Claudin 6 is also a global marker of definitive endoderm. Directly interacts with TJP1/ZO-1, TJP2/ZO-2 and TJP3/ZO-3.
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Database links
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The links below go to external sites and will open in a new browser window |
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Raised in
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Rabbit
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Clonality
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Polyclonal
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Isotype
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IgG
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Purity
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Immunogen affinity purified
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Storage buffer
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Preservative: 0.02% Sodium Azide
Constituents: 50% Glycerol, PBS, 150mM Sodium chloride, pH 7.4 Material safety datasheets (MSDS) for this product:
Glycerol MSDS
Sodium Azide MSDS
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Purification notes
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The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.
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Form
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Liquid
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Concentration
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1.000 mg/ml
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Storage instructions
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Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
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At Abcam, we have one centralized database to hold all of our product information, so that everything we know about this Claudin 6 (phospho Y219) antibody is on this datasheet. But please do contact us if you would like any reassurance! |
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See below for Claudin 6 (phospho Y219) antibody images, references, products related to ab78347 and other tools.
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Claudin 6 (phospho Y219) antibody images:
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 Western blot - Claudin 6 (phospho Y219) antibody (ab78347)
All lanes : Claudin 6 (phospho Y219) antibody (ab78347) at 1/500 dilution
Lane 1 : Extracts from RAW264.7 cells,
treated with UV (5mins)
Lane 2 : Extracts from RAW264.7 cells,
treated with UV (5mins) with Immunising peptide at 10 µg
Lysates/proteins at 30 µg per lane.
Predicted band size : 23 kDa
Observed band size : 23 kDa
Additional bands at : 30 kDa. We are unsure as to the identity of these extra bands.
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Search PubMed (MEDLINE) for references to Claudin 6
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Claudin 6 (phospho Y219) antibody - more information
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Claudin 6 (phospho Y219) antibody - related products:
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