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Products:Microbiology >> Interspecies Interaction >> Host Virus Interaction
Overview
Product name
Anti-Claudin 6 (phospho Y219) antibody
See all Claudin 6 products (5) ...
Description
Rabbit polyclonal to Claudin 6 (phospho Y219)
Specificity
This antibody detects endogenous levels of Claudin 6 only when phosphorylated at tyrosine 219.
Tested applications
ELISA, WBmore details
Cross reactivity
Reacts with
Mouse, Human
Immunogen
Synthesized phosphopeptide derived from human Claudin 6 around the phosphorylation site of tyrosine 219.
Positive control
Extracts from RAW264.7 cells.
Properties
Form
Liquid
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage buffer
Preservative: 0.02% Sodium Azide
Constituents: 50% Glycerol, PBS, 150mM Sodium chloride, pH 7.4
Concentration
Concentration information loading...
Purity
Immunogen affinity purified
Purification notes
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.
Clonality
Polyclonal
Isotype
IgG
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Western blot - Claudin 6 (phospho Y219) antibody (ab78347)
(enlarge)
Applications
Show applications key
Our Abpromise guarantee covers the use of ab78347 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
ELISA
ELISA: 1/5000ELISA: 1/5000
WB: 1/500 - 1/1000.Detects a band of approximately 23 kDa (predicted molecular weight: 23 kDa).
Target
Function
Plays a major role in tight junction-specific obliteration of the intercellular space.
Sequence similarities
Belongs to the claudin family.
Cellular localization
Cell junction > tight junction. Cell membrane.
Target information above from: UniProt accessionP56747
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Alternative names
- Claudin-6 antibody
- Claudin6 antibody
- CLD6_HUMAN antibody
see all
Database links
- Entrez Gene: 9074 Human
- Entrez Gene: 54419 Mouse
- SwissProt: P56747 Human
- SwissProt: Q9Z262 Mouse
- Unigene: 533779 Human
- Unigene: 485133 Mouse
- Unigene: 86421 Mouse
Anti-Claudin 6 (phospho Y219) antibody images:
Western blot - Claudin 6 (phospho Y219) antibody (ab78347)
All lanes : Anti-Claudin 6 (phospho Y219) antibody (ab78347) at 1/500 dilution
Lane 1 : Extracts from RAW264.7 cells,
treated with UV (5mins)
Lane 2 : Extracts from RAW264.7 cells,
treated with UV (5mins) with Immunising peptide at 10 µg
Lysates/proteins at 30 µg per lane.
Predicted band size : 23 kDa
Observed band size : 23 kDa
Additional bands at : 30 kDa. We are unsure as to the identity of these extra bands.
References for Anti-Claudin 6 (phospho Y219) antibody (ab78347)
ab78347 has not yet been referenced specifically in any publications.
Publishing research using ab78347? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"