Anti-Claudin 8 antibody (ab211439)
Key features and details
- Rabbit polyclonal to Claudin 8
- Suitable for: IHC-P, WB, Flow Cyt (Intra)
- Reacts with: Human
- Isotype: IgG
Get better batch-to-batch reproducibility with a recombinant antibody
- Research with confidence – consistent and reproducible results with every batch
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- Success from the first experiment – confirmed specificity through extensive validation
- Ethical standards compliant – production is animal-free
Overview
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Product name
Anti-Claudin 8 antibody
See all Claudin 8 primary antibodies -
Description
Rabbit polyclonal to Claudin 8 -
Host species
Rabbit -
Tested applications
Suitable for: IHC-P, WB, Flow Cyt (Intra)more details -
Species reactivity
Reacts with: Human -
Immunogen
Synthetic peptide within Human Claudin 8 aa 100-200 (internal sequence) conjugated to keyhole limpet haemocyanin. The exact immunogen sequence used to generate this antibody is proprietary information. If additional detail on the immunogen is needed to determine the suitability of the antibody for your needs, please contact our Scientific Support team to discuss your requirements.
Database link: P56748 -
Positive control
- IHC-P: Human colon tissue. WB: 293 cell lysate. Flow Cyt (Intra): 293 cells.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.4
Preservative: 0.09% Sodium azide
Constituent: 99% PBS -
Concentration information loading...
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Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Recombinant Protein
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Related Products
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab211439 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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IHC-P |
1/75. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
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WB |
1/1000. Predicted molecular weight: 25 kDa.
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Flow Cyt (Intra) |
1/10 - 1/50.
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Notes |
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IHC-P
1/75. Perform heat mediated antigen retrieval before commencing with IHC staining protocol. |
WB
1/1000. Predicted molecular weight: 25 kDa. |
Flow Cyt (Intra)
1/10 - 1/50. |
Target
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Function
Plays a major role in tight junction-specific obliteration of the intercellular space. -
Tissue specificity
Expressed in the epididymis, mainly in the caput segment. -
Sequence similarities
Belongs to the claudin family. -
Cellular localization
Cell junction > tight junction. Cell membrane. Localizes to tight junctions in all 3 segments of the epididymis, in the caput found in the lateral margins of principal cells, and in the corpus at the interface between basal and principal cells. - Information by UniProt
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Database links
- Entrez Gene: 9073 Human
- Omim: 611231 Human
- SwissProt: P56748 Human
- Unigene: 162209 Human
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Alternative names
- Claudin 8 antibody
- Claudin-8 antibody
- CLD8_HUMAN antibody
see all
Images
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Immunohistochemical analysis of formalin-fixed, paraffin-embedded Human colon tissue labeling Claudin 8 with ab211439 at 1/75 dilution.
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Anti-Claudin 8 antibody (ab211439) at 1/1000 dilution + 293 cell lysate at 35 µg
Predicted band size: 25 kDa -
Intracellular flow cytometric analysis of 293 cells labeling Claudin 8 with ab211439 at 1/10 dilution (right histogram) compared to a negative control (left histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
Protocols
Datasheets and documents
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Datasheet download
References (2)
ab211439 has been referenced in 2 publications.
- Zhu Z et al. Prognostic Value and Potential Biological Functions of CLDN8 in Patients with Clear Cell Renal Cell Carcinoma. Onco Targets Ther 13:9135-9145 (2020). PubMed: 32982302
- Liu B et al. MiR-361-5p inhibits cell proliferation and induces cell apoptosis in retinoblastoma by negatively regulating CLDN8. Childs Nerv Syst 35:1303-1311 (2019). PubMed: 31161266