Anti-Cleaved PARP antibody [4B5BD2] (ab110315)

  Immunocytochemistry/ Immunofluorescence - Cleaved PARP antibody [4B5BD2] (ab110315)

Immunocytochemistry images of stained untreated (A) and 4 hours 1 µM Staurosporine-treated (B) Human HeLa cells. The cells were paraformaldehyde fixed (4%, 20 minutes) and Triton X-100 permeabilized (0.1%, 15 minutes). The cells were incubated with 1.0 µg/ml ab110315 for 2 hours at room temperature or over night at 4°C. 10% goat serum was used as the blocking agent for all blocking steps. The secondary antibody was Alexa Fluor® 488 goat anti-mouse IgG (H+L) (in green) used at 2.0 µg/ml for 2 hours. DAPI was used to stain the cell nuclei (in red). Heat induced antigen retrieval (0.1 M Tris-HCl, 5% urea, pH 9.5 for 5 min at 95°C) improves signal. Note that the ab110315 labels only condensed and/or fragmented nuclei of apoptotic Staurosporine-treated cells.

  Western blot - Anti-Cleaved PARP antibody [4B5BD2] (ab110315)

Lanes 1 - 2 : Antibody that recognizes full-length PARP1
Lanes 3 - 4 : Anti-Cleaved PARP antibody [4B5BD2] (ab110315) at 1 µg/ml

Lane 1 : untreated HeLa cells
Lane 2 : HeLa cells treated with 1 µM Staurosporinefor 4 hours
Lane 3 : untreated HeLa cells
Lane 4 : HeLa cells treated with 1 µM Staurosporinefor 4 hours

Lysates/proteins at 20 µg per lane.


Predicted band size : 113 kDa


Western Blot analysis using ab110315 antibody and 20 µg of untreated (CON) or 4 hours 1 µM Staurosporine-treated (STS) HeLa cells. Blots were incubated with an antibody that recognizes both the full-length PARP-1 and its 89 kDa fragment (left panel), or 1.0 µg/mL PARP-1 (cleaved) antibody (ab110315) (right panel). Appropriate HRP-conjugated secondary antibodies followed by ECL detection were used. Note that the MS777 antibody recognizes the apoptosis-specific 89 kDa fragment of PARP-1 but it does not recognize the full-length PARP-1.

  In-Cell ELISA - Cleaved PARP antibody [4B5BD2] (ab110315)

In-Cell ELISA (ICE) using ab110315 on HeLa cells treated with Staurosporine to induce apoptosis. HeLa cells were seeded overnight (50,000 cells/well), treated for 4 hours with 1 µM Staurosporine or with the drug vehicle (DMSO), fixed for Detaching Adherent Cells and analyze.

  Flow Cytometry - Cleaved PARP antibody [4B5BD2] (ab110315)

Flow cytometry analysis of apoptosis using ab110315. HL-60 cells were treated with 1 µM Staurosporin for 4 hours (blue) or vehicle control (red). Control cells were also stained with an equal amount of an isotype control antibody (black).