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Products:Cell Biology >> Apoptosis >> Nucleus >> PARP

Anti-Cleaved PARP antibody [E51] (ab32064)

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2 questions for ab32064

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Question 1

Monday 21-May-2012

How was this antibody validated for IF in IHC-P?

ANSWER:

  

Thank you for contacting Abcam regarding ab32064.


I have confirmed with the laboratory that we currently do not have any data demonstrating the use of this antibody with a fluorescently conjugated secondary antibody. However, we expect that the antibody will work as well with a fluorescently conjugated antibody as it would with an enzymatic label.


I hope this information is helpful. Please do not hesitate to contact me if you have any additional questions.

Question 2

Wednesday 28-September-2011

Dear Sir/Madam

The customer would like to PARP antibody that result same with the publication (page 1387, attach file) which have PARP protein and cleave form. So, please kindly recommend abcam antibody.

ANSWER:

  

Thank you for contacting us.

We have several antibodies which can be used to detect PARP in Western blotting. From the study I believe you wish to detect both the cleaved (C-terminal fragment) and the full length PARP form in mouse samples? This can be done using ab75757 (Chicken polyclonal) or ab37722 (Rabbit polyclonal). Although ab37722 has not been tested with mouse samples due but to the similarity in immunogen sequence (100%) it is predicted to react.

If however you are interested in human samples this can be achieved with ab32071 (Rabbit monoclonal [E78]) which was raised against a synthetic peptide of the C-teminal residues of human PARP. Please note this antibody does not react with mouse samples.

We also have several antibodies that specifically recognise the cleavage product of PARP such as ab4830 (reacts with human C-terminal PARP) and ab32064 (reacts with mouse and human PARP, N-terminal).

I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

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