Anti-Cleaved PARP antibody [E51] (ab32064)

  Western blot - cleaved PARP antibody [E51] (ab32064)

All lanes : Anti-Cleaved PARP antibody [E51] (ab32064) at 1/1000000 dilution

Lane 1 : Jurkat cell lysate. Untreated.
Lane 2 : Jurkat cell lysate. Treated with Camptothecin.


Predicted band size : 25 kDa
Observed band size : 25 kDa

  Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - cleaved PARP antibody [E51] (ab32064)

ab32064 at a 1:100 dilution staining PARP cleaved p25 in human breast carcinoma tissue, using Immunohistochemistry, Paraffin Embedded Tissue.

  Flow Cytometry-Cleaved PARP antibody [E51](ab32064)

Overlay histogram showing Jurkat cells stained with ab32064 (red line). The cells were fixed with methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab32064, 1/20 dilution) for 30 min at 22°C. The secondary antibody used was DyLight® 488 goat anti-rabbit IgG (H+L) (ab96899) at 1/500 dilution for 30 min at 22°C. Isotype control antibody (black line) was rabbit monoclonal IgG (0.5µg/1x10⁶ cells) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a slightly decreased signal in Jurkat cells fixed with 4% paraformaldehyde (10 min)/permeabilized in 0.1% PBS-Tween used under the same conditions.