Immunoprecipitation abreview for Anti-Cleaved PARP antibody [E51]

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Application Immunoprecipitation
Sample Human Cell lysate - whole cell (MCF7)
Total protein in input 50 µg
Treatment T3 100 micro moles for 48 hrs
Immuno-precipitation step Staph. aureus
Specification MCF7

Other product details

Incubation time 3 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: RIPA BUFFER
Concentration 2 µg/mg lysate

Western Blot antibody

Dilution 1/50000
Name Non-Abcam antibody was used: goat anti rabbit antibody
Host species: Goat
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase

Additional data

Additional Notes Prepared whole cell lysate by using RIPA buffer and made it up to 1 microgram per micro litre and add beads for preclearing and centrifuged it and supernatant has taken and pimary antibody added to that and rotated it for 3 hrs and beads was added and rotated for 1 hr and centrifused it and beads were washes with pbs thrice and the protein is isolated by using protein loading dye and subjected to immunoblting
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Submitted Jun 27 2013