Immunoprecipitation abreview for Anti-Cleaved PARP antibody [E51]

Abcam has not validated the combination of species/application used in this Abreview.
Application Immunoprecipitation
Sample Human Cell lysate - whole cell (MCF7)
Total protein in input 50 µg
Treatment T3 100 micro moles for 48 hrs
Immuno-precipitation step Staph. aureus
Specification MCF7

Other product details

Incubation time 3 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: RIPA BUFFER
Concentration 2 µg/mg lysate

Western Blot antibody

Dilution 1/50000
Name Non-Abcam antibody was used: goat anti rabbit antibody
Host species: Goat
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase

Additional data

Additional Notes Prepared whole cell lysate by using RIPA buffer and made it up to 1 microgram per micro litre and add beads for preclearing and centrifuged it and supernatant has taken and pimary antibody added to that and rotated it for 3 hrs and beads was added and rotated for 1 hr and centrifused it and beads were washes with pbs thrice and the protein is isolated by using protein loading dye and subjected to immunoblting

Abcam user community

Verified customer

Submitted Jun 27 2013