Western blot abreview for Anti-Cleaved PARP antibody [E51]

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Application Western blot
Loading amount 50 µg
Gel Running Conditions Reduced Denaturing (10)
Sample Human Cell lysate - whole cell (MCF7)
Specification MCF7
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 37°C

Other product details

Incubation time 16 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: skimmed milk 5%
Dilution 1/5000

Secondary antibody

Name Non-Abcam antibody was used: goat anti rabbit antibody from sigma
Host species: Goat
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Dilution 1/25000


Detection method ECL
Bands Specific: 25 kDa
Exposure 15 minute(s) and 0 second(s)

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Submitted Sep 09 2013