Immunocytochemistry abreview for Anti-Cleaved PARP1 antibody [E51]

Excellent
Abreviews
Application
Immunocytochemistry
Sample
Human Cultured Cells (Ovarian cancer cell line)
Permeabilization
Yes - 0.25% tritonx X-100
Specification
Ovarian cancer cell line
Blocking step
Background buster as blocking agent for 30 minute(s) · Concentration: 100% · Temperature: 25°C
Fixative
Formaldehyde

Other product details

Incubation time
18 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: SignalStain(R) Ab diluent
Dilution
1/250

Secondary antibody

Name
Non-Abcam antibody was used: SignalStain­ Boost IHC Detection Reagent (HRP, Rab
Host species: Goat
Clonality: Monoclonal
Conjugation: Horse Radish Peroxidase

Additional data

Additional Notes
Counter-stained with Hematoxylin (blue)
Username

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Submitted Aug 11 2014

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