Immunocytochemistry abreview for Anti-Cleaved PARP antibody [E51]

Application Immunocytochemistry
Blocking step Background buster as blocking agent for 30 minute(s) · Concentration: 100% · Temperature: 25°C
Sample Human Cultured Cells (Ovarian cancer cell line)
Specification Ovarian cancer cell line
Permeabilization Yes - 0.25% tritonx X-100
Fixative Formaldehyde

Other product details

Incubation time 18 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: SignalStain(R) Ab diluent
Dilution 1/250

Secondary antibody

Name Non-Abcam antibody was used: SignalStain­ Boost IHC Detection Reagent (HRP, Rab
Host species: Goat
Clonality: Monoclonal
Conjugation: Horse Radish Peroxidase

Additional data

Additional Notes Counter-stained with Hematoxylin (blue)

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Submitted Aug 11 2014