Western blot abreview for Anti-Cleaved PARP1 antibody [E51]

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Abreviews
Application
Western blot
Sample
Zebrafish Tissue lysate - whole (whole embryo)
Gel Running Conditions
Reduced Denaturing (12%)
Loading amount
10 µg
Treatment
25uM tamoxifen
Specification
whole embryo
Blocking step
BSA as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C

Other product details

Incubation time
24 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: PBST 2%BSA
Dilution
1/10000

Secondary antibody

Dilution
1/1000
Name
Non-Abcam antibody was used: donkey anti-Rabbit IgG (H+L) Cross Adsorbed Second
Host species: Goat
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase

Detection

Detection method
ECL+
Exposure
10 minute(s) and 0 second(s)
Bands
Specific: 25 kDa Non-specific: 10, 12 kDa
Positive control
human HEK293T cells untreated and treated with doxorubinin (lanes

Additional data

Additional Notes
ABTRIAL-EBLLK We have tried additional conditions: dilution 1:10000, buffer without BSA, dilution 1:1000 (PBST 2%BSA) We could not find the conditions for this antibody to give reproducible results for zebrafish. For human samples works well. Lane 1. HEK297 untreated Lane 2. HEK297 treated doxorubicin Lane 3. Zebrafish whole body lysate untreated Lane 4. Zebrafish whole body lysate treated tamoxifen Lane 5. Zebrafish whole body lysate treated tamoxifen and cisplatin
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Submitted Jul 28 2015

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