Western blot abreview for Anti-Cleaved PARP antibody [E51]

Application Western blot
Sample Human Cell lysate - whole cell (U2OS cells)
Gel Running Conditions Reduced Denaturing (4-12% Gradient Gel)
Loading amount 20 µg
Specification U2OS cells
Blocking step Licor Blocking Buffer as blocking agent for 30 minute(s) · Concentration: 100% · Temperature: 25°C

Other product details

Incubation time 2 hour(s) and 0 minute(s) · Temperature: 25°C · Diluent: Licor Blocking Buffer
Dilution 1/1000

Secondary antibody

Dilution 1/5000
Name Non-Abcam antibody was used: Alexa Fluor 680 anti-Rabbit antibody
Host species: Goat
Clonality: Polyclonal
Conjugation: Alexa Fluor­ 680


Detection method Licor
Exposure 10 minute(s) and 0 second(s)
Bands Specific: 25 kDa
Positive control Whole cell lysate from U2OS cells

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Submitted Mar 02 2017