Western blot abreview for Anti-Cleaved PARP1 antibody [E51]

Excellent
Abreviews
Application
Western blot
Sample
Human Cell lysate - whole cell (U2OS cells)
Gel Running Conditions
Reduced Denaturing (4-12% Gradient Gel)
Loading amount
20 µg
Specification
U2OS cells
Blocking step
Licor Blocking Buffer as blocking agent for 30 minute(s) · Concentration: 100% · Temperature: 25°C

Other product details

Incubation time
2 hour(s) and 0 minute(s) · Temperature: 25°C · Diluent: Licor Blocking Buffer
Dilution
1/1000

Secondary antibody

Dilution
1/5000
Name
Non-Abcam antibody was used: Alexa Fluor 680 anti-Rabbit antibody
Host species: Goat
Clonality: Polyclonal
Conjugation: Alexa Fluor­ 680

Detection

Detection method
Licor
Exposure
10 minute(s) and 0 second(s)
Bands
Specific: 25 kDa
Positive control
Whole cell lysate from U2OS cells
Username

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Submitted Mar 02 2017

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