Anti-Clostridium botulinum E Toxoid antibody [209F2] (ab40787)
Key features and details
- Mouse monoclonal [209F2] to Clostridium botulinum E Toxoid
- Suitable for: ICC/IF, Inhibition Assay
- Reacts with: Clostridium botulinum
- Isotype: IgG
Overview
-
Product name
Anti-Clostridium botulinum E Toxoid antibody [209F2] -
Description
Mouse monoclonal [209F2] to Clostridium botulinum E Toxoid -
Host species
Mouse -
Specificity
BoTox-E (2-17) 100%; BoTox-E 100% Does not cross react with BoTox-A, BoTox-B, BoTox-C or BoTox-F (1-16) -
Tested applications
Suitable for: ICC/IF, Inhibition Assaymore details -
Species reactivity
Reacts with: Clostridium botulinum -
Immunogen
Synthetic peptide analogue of the amino terminal region of BoTox-E.
-
General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
-
Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
Storage buffer
Constituent: Tissue culture supernatant -
Concentration information loading...
-
Purity
Tissue culture supernatant -
Purification notes
Sterile filtered culture supernatant. -
Clonality
Monoclonal -
Clone number
209F2 -
Isotype
IgG -
Research areas
Associated products
-
Compatible Secondaries
-
Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab40787 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
---|---|---|
ICC/IF | ||
Inhibition Assay |
Notes |
---|
This product has been found to stain Neuro2A cells treated with GT1b ganglioside prior to the addition of BoTox-E at a dilution of 1:50 using an indirect immunocytochemical staining procedure.
Inhib: Use at an assay dependent dilution.
This product has been found to inhibit the entry of BoTox-E to Neuro2A cells treated with GT1b ganglioside prior to the addition of the toxin.
With this antibody, we have found that blocking with 5% goat or donkey serum significantly reduces background as compared to BSA or milk.
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
Target
-
Relevance
Clostridium botulinum E Toxoid acts by inhibiting neurotransmitter release. It binds to peripheral neuronal synapses, is internalized and moves by retrograde transport up the axon into the spinal cord where it can move between postsynaptic and presynaptic neurons. It inhibits neurotransmitter release by acting as a zinc endopeptidase. It has a non-toxic component which is necessary to maintain toxicity. -
Cellular localization
Secreted -
Database links
- SwissProt: Q00496 Clostridium botulinum
-
Alternative names
- BoNT/E antibody
- Bontoxilysin e antibody
- Botulinum neurotoxin type e non toxic component antibody
see all
Protocols
Datasheets and documents
-
Datasheet download
References (1)
ab40787 has been referenced in 1 publication.
- Shu Y et al. Phosphorylation of SNAP-25 at Ser187 mediates enhancement of exocytosis by a phorbol ester in INS-1 cells. J Neurosci 28:21-30 (2008). PubMed: 18171919