Anti-Clostridium tetani toxin IgG Human ELISA Kit (ab108771)
- Product nameAnti-Clostridium tetani toxin IgG Human ELISA Kit
- Detection methodColorimetric
Intra-assay Sample n Mean SD CV% Pos. Serum 24 1.24U/ml 4.9%
- Tests1 x 96 test
- Sample typeSerum, Plasma
- Assay typeIndirect
- Sensitivity< 0.05 U/ml
- Range0.05 U/ml - 1 U/ml
- Assay durationMultiple steps standard assay
- Species reactivityReacts with: Human
- Product overview
Abcam’s anti-Clostridium tetani toxin IgG Human in vitro ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for the accurate quantitative measurement of IgG class antibodies against Clostridium tetani toxin in Human serum and plasma.
A 96-well plate has been precoated with Clostridium tetani toxin antigens to bind cognate antibodies. Controls or test samples are added to the wells and incubated. Following washing, a horseradish peroxidase (HRP) labelled anti-Human IgG conjugate is added to the wells, which binds to the immobilized Clostridium tetani toxin-specific antibodies. TMB is then catalyzed by the HRP to produce a blue color product that changes to yellow after adding an acidic stop solution. The density of yellow coloration is directly proportional to the amount of Clostridium tetani toxin IgG sample captured in plate.
- Tested applicationsIndirect ELISA more details
- Storage instructionsStore at +4°C. Please refer to protocols.
Components Identifier 1 x 96 tests 20X Washing Solution White cap 1 x 50ml Clostridium tetani toxin (IgG) Coated Microplate (12 x 8 wells) 12 strips of 8 wells 1 unit Clostridium tetani toxin anti-IgG HRP Conjugate Blue, Black cap 1 x 20ml Clostridium tetani toxin IgG Standard A – 0.0 U/mL Blue cap 1 x 2ml Clostridium tetani toxin IgG Standard B – 0.1 U/mL Green Cap 1 x 2ml Clostridium tetani toxin IgG Standard C – 0.5 U/mL Yellow cap 1 x 2ml Clostridium tetani toxin IgG Standard D – 1.0 U/mL Red cap 1 x 2ml Cover foil 1 unit IgG Sample Diluent Yellow, White cap 1 x 100ml Stop Solution Red cap 1 x 15ml Strip holder 1 unit TMB Substrate Solution Yellow cap 1 x 15ml
- Research Areas
- Clostridium tetani toxin IgG
- Tetanus Toxin IgG
Our Abpromise guarantee covers the use of ab108771 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Anti-Clostridium tetani toxin IgG Human ELISA Kit images
Representative Standard Curve using ab108771
References for Anti-Clostridium tetani toxin IgG Human ELISA Kit (ab108771)
ab108771 has not yet been referenced specifically in any publications.