Anti-CoCoA antibody (ab70564)
Key features and details
- Rabbit polyclonal to CoCoA
- Suitable for: WB, IP
- Reacts with: Mouse, Human
- Isotype: IgG
Overview
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Product name
Anti-CoCoA antibody
See all CoCoA primary antibodies -
Description
Rabbit polyclonal to CoCoA -
Host species
Rabbit -
Tested applications
Suitable for: WB, IPmore details -
Species reactivity
Reacts with: Mouse, Human
Predicted to work with: Rat, Rabbit, Horse, Guinea pig, Dog, Chimpanzee, Rhesus monkey, Gorilla, Chinese hamster, Orangutan, Elephant -
Immunogen
Synthetic peptide corresponding to Human CoCoA (C terminal). Synthetic peptide corresponding to a region between residue 650 and the C terminus (residue 691) of human CoCoA (NP_065949.1)
Database link: Q9P1Z2 -
Positive control
- Whole cell lysate from: HeLa, 293T, NIH3T3, Ramos and MCF7 cells.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
Storage buffer
pH: 7
Preservative: 0.09% Sodium azide
Constituents: 1.815% Tris, 1.764% Sodium citrate, 0.021% PBS -
Concentration information loading...
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Purity
Immunogen affinity purified -
Purification notes
ab70564 was affinity purified using an epitope specific to CoCoA immobilized on solid support. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Positive Controls
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab70564 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB |
1/1000 - 1/5000. Detects a band of approximately 90 kDa (predicted molecular weight: 77 kDa).
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IP |
Use at 2-5 µg/mg of lysate.
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Notes |
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WB
1/1000 - 1/5000. Detects a band of approximately 90 kDa (predicted molecular weight: 77 kDa). |
IP
Use at 2-5 µg/mg of lysate. |
Target
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Relevance
Functions as a coactivator for aryl hydrocarbon and nuclear receptors (NR). Recruited to promoters through its contact with the N terminal basic helix-loop-helix-Per-Arnt-Sim (PAS) domain of transcription factors or coactivators, such as NCOA2. During ER-activation acts synergistically in combination with other NCOA2-binding proteins, such as EP300, CREBBP and CARM1. Involved in the transcriptional activation of target genes in the Wnt/CTNNB1 pathway. Functions as a secondary coactivator in LEF1 mediated transcriptional activation via its interaction with CTNNB1. Coactivator function for nuclear receptors and LEF1/CTNNB1 involves differential utilization of two different activation regions. -
Cellular localization
Cytoplasm. Nucleus. Note: shuttles between nucleus and cytoplasm. -
Database links
- Entrez Gene: 57658 Human
- Entrez Gene: 67488 Mouse
- Entrez Gene: 246047 Rat
- SwissProt: Q9P1Z2 Human
- SwissProt: Q8CGU1 Mouse
- SwissProt: Q66HR5 Rat
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Alternative names
- Calcium binding and coiled coil domain 1 antibody
- CALCOCO1 antibody
- Calphoglin antibody
see all
Images
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All lanes : Anti-CoCoA antibody (ab70564) at 0.4 µg/ml
Lane 1 : HeLa whole cell lysate
Lane 2 : 293T whole cell lysate
Lane 3 : NIH3T3 whole cell lysate
Lane 4 : Ramos whole cell lysate
Lane 5 : MCF7 whole cell lysate
Lysates/proteins at 50 µg per lane.
Developed using the ECL technique.
Predicted band size: 77 kDa
Observed band size: 90 kDa why is the actual band size different from the predicted?
Exposure time: 3 minutes -
Detection of human CoCoA by Immunoprecipitation in whole cell lysate from MCF7 cells (1 mg, 20% of immunoprecipitate loaded/lane), using ab70564 at 3 µg/mg of lysate (lane 2).
CoCoA was also immunoprecipitated using another CoCoA antibody (ab70563) which recognizes an upstream epitope (lane 1).
Lane 3: Control IgG immunoprecipitate.
Subsequent blotting of immunoprecipitate for the detection of CoCoA was performed using 1µg/ml of ab70564 and 50µg of lysate.
Detection was by chemiluminescence with an exposure time of 30 seconds.
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (2)
ab70564 has been referenced in 2 publications.
- Stefely JA et al. Mass spectrometry proteomics reveals a function for mammalian CALCOCO1 in MTOR-regulated selective autophagy. Autophagy 16:2219-2237 (2020). PubMed: 31971854
- Karere GM et al. Identification of candidate genes encoding an LDL-C QTL in baboons. J Lipid Res 54:1776-85 (2013). PubMed: 23596326