Recombinant Anti-CoREST antibody [EPR13826(B)] (ab181139)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR13826(B)] to CoREST
- Suitable for: Flow Cyt (Intra), WB
- Reacts with: Human
Related conjugates and formulations
Overview
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Product name
Anti-CoREST antibody [EPR13826(B)]
See all CoREST primary antibodies -
Description
Rabbit monoclonal [EPR13826(B)] to CoREST -
Host species
Rabbit -
Tested applications
Suitable for: Flow Cyt (Intra), WBmore details -
Species reactivity
Reacts with: Human -
Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- Jurkat, HeLa and MOLT4 cell lysates; Jurkat cells.
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
Preservative: 0.01% Sodium azide
Constituents: 40% Glycerol (glycerin, glycerine), 59% PBS, 0.05% BSA -
Concentration information loading...
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Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR13826(B) -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Isotype control
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Positive Controls
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab181139 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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Flow Cyt (Intra) |
1/150.
ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody. |
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WB |
1/1000 - 1/10000. Detects a band of approximately 66 kDa (predicted molecular weight: 53 kDa).
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Notes |
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Flow Cyt (Intra)
1/150. ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody. |
WB
1/1000 - 1/10000. Detects a band of approximately 66 kDa (predicted molecular weight: 53 kDa). |
Target
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Function
Essential component of the BHC complex, a corepressor complex that represses transcription of neuron-specific genes in non-neuronal cells. The BHC complex is recruited at RE1/NRSE sites by REST and acts by deacetylating and demethylating specific sites on histones, thereby acting as a chromatin modifier. In the BHC complex, it serves as a molecular beacon for the recruitment of molecular machinery, including MeCP2 and SUV39H1, that imposes silencing across a chromosomal interval. Plays a central role in demethylation of Lys-4 of histone H3 by promoting demethylase activity of KDM1A on core histones and nucleosomal substrates. It also protects KDM1A from the proteasome. -
Tissue specificity
Ubiquitously expressed. -
Sequence similarities
Belongs to the CoREST family.
Contains 1 ELM2 domain.
Contains 2 SANT domains. -
Domain
The SANT domains may bridge the nucleosomal substrates and the demethylase KDM1A. -
Post-translational
modificationsPhosphorylated by HSV-1 protein kinases in case of infection. -
Cellular localization
Nucleus. Upon infection by HSV-1, it is partially translocated into the cytoplasm in an HSV-1-dependent manner. - Information by UniProt
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Database links
- Entrez Gene: 23186 Human
- Omim: 607675 Human
- SwissProt: Q9UKL0 Human
- Unigene: 510521 Human
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Alternative names
- COREST antibody
- KIAA0071 antibody
- Nr2b2 antibody
see all
Images
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All lanes : Anti-CoREST antibody [EPR13826(B)] (ab181139) at 1/2000 dilution
Lane 1 : Jurkat cell lysate
Lane 2 : HeLa cell lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugate at 1/1000 dilution
Predicted band size: 53 kDa
Observed band size: 66 kDa why is the actual band size different from the predicted? -
Anti-CoREST antibody [EPR13826(B)] (ab181139) at 1/10000 dilution + MOLT4 cell lysate at 20 µg
Secondary
Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugate at 1/1000 dilution
Predicted band size: 53 kDa
Observed band size: 66 kDa why is the actual band size different from the predicted? -
Intracellular flow cytometric analysis of 2% paraformaldehyde-fixedJurkat cells labeling CoREST with ab181139 at 1/150 (red) compared to a Rabbit monoclonal IgG isotype control (green), followed by Goat anti rabbit IgG (FITC) secondary antibody at 1/150 dilution.
Datasheets and documents
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SDS download
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Datasheet download
Certificate of Compliance
References (0)
ab181139 has not yet been referenced specifically in any publications.