Coenzyme A Detection Kit (ab102504)
- Product nameCoenzyme A Detection KitSee all Coenzyme A kits ...
- Tests100 x 1 assay
- Sample typeUrine, Serum, Plasma, Other biological fluids, Tissue Extracts, Cell Lysate, Cell culture media
- Assay typeQuantitative
- Sensitivity> 2.5 µM
- Range2.5 µM - 250 µM
- Assay time1h 00m
- Product overview
Abcam's Coenzyme A Detection Kit provides an easy, accurate assay to measure the CoA level in variety biological samples. In the assay, free CoA is specifically utilized to generate products which react with OxiRed Probe to generate color (λ = 570 nm) and fluorescence (Ex=535/Em=587 nm). The assay can detect 0.1 to 10 nmol of CoA (2.5-250 µM concentration range) in a variety of samples.
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Coenzyme A (CoA) is composed of units derived from cysteine, pantothenic acid, and ATP. It plays important roles in the synthesis and oxidation of fatty acids, pyruvate oxidation in the citric acid cycle and many other biological processes. One of the main functions of CoA is the carrying and transfer of acyl groups. One of the most important acyl groups transferred is the acetate group, in which case the molecule is called acetyl-CoA. The acetyl group eventually finds itself incorporated into a variety of molecules such as cholesterol, acetylcholine, melatonin, heme and the TCA cycle intermediates.
- Tested applicationsFunctional Studies more details
- Storage instructionsStore at -20°C. Please refer to protocols.
Components Identifier 100 tests Acyl CoA Developer Green 1 unit CoA Assay Buffer WM 1 x 25ml CoA Standard Yellow 1 unit CoA Substrate Purple 1 x 1ml Conversion Enzyme Mix Blue 1 unit OxiRed Probe (in DMSO) Red 1 unit
- Pathways and Processes
- Metabolic signaling pathways
- Lipid and lipoprotein metabolism
- Lipid metabolism
Our Abpromise guarantee covers the use of ab102504 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
References for Coenzyme A Detection Kit (ab102504)
ab102504 has not yet been referenced specifically in any publications.