Overview

  • Product nameCoenzyme A Detection Kit
    See all Coenzyme A kits
  • Tests
    100 x 1 assay
  • Sample type
    Urine, Serum, Plasma, Other biological fluids, Tissue Extracts, Cell Lysate, Cell culture media
  • Assay typeQuantitative
  • Sensitivity
    > 2.5 µM
  • Range
    2.5 µM - 250 µM
  • Assay time
    1h 00m
  • Product overview

    Abcam's Coenzyme A Detection Kit provides an easy, accurate assay to measure the CoA level in variety biological samples. In the assay, free CoA is specifically utilized to generate products which react with OxiRed Probe to generate color (λ = 570 nm) and fluorescence (Ex=535/Em=587 nm). The assay can detect 0.1 to 10 nmol of CoA (2.5-250 µM concentration range) in a variety of samples.
    Visit our FAQs page for tips and troubleshooting.

  • Notes

    Coenzyme A (CoA) is composed of units derived from cysteine, pantothenic acid, and ATP. It plays important roles in the synthesis and oxidation of fatty acids, pyruvate oxidation in the citric acid cycle and many other biological processes. One of the main functions of CoA is the carrying and transfer of acyl groups. One of the most important acyl groups transferred is the acetate group, in which case the molecule is called acetyl-CoA. The acetyl group eventually finds itself incorporated into a variety of molecules such as cholesterol, acetylcholine, melatonin, heme and the TCA cycle intermediates.

  • Tested applicationsFunctional Studiesmore details

Properties

  • RelevanceCoenzyme A (CoA) is composed of units derived from cysteine, pantothenic acid, and ATP. It plays important roles in the synthesis and oxidation of fatty acids, pyruvate oxidation in the citric acid cycle and many other biological processes. One of the main functions of Coenzyme A is the carrying and transfer of acyl groups. One of the most important acyl groups transferred is the acetate group, in which case the molecule is called acetyl-Coenzyme A. The acetyl group eventually finds itself incorporated into a variety of molecules such as cholesterol, acetylcholine, melatonin, heme and the TCA cycle intermediates.
  • Alternative names
    • CoA
    • CoASH
    • HSCoA

Applications

Our Abpromise guarantee covers the use of ab102504 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Functional Studies Use at an assay dependent dilution.

Coenzyme A Detection Kit images

  • Example of Standard Curve obtained using ab102504.

  • Example of Standard Curve obtained using ab102504.

Protocols

References for Coenzyme A Detection Kit (ab102504)

ab102504 has not yet been referenced specifically in any publications.

Product Wall

Thank you for the information, and the clarification. I am not sure we have any data for precision, which would tell us how well the assay could distinguish among differences. The low standard, 0.2 nmol per well in the fluorescent version of the assay,...

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Thank you for your interest in the Coenzyme A assay, ab102504. The detection limit of the fluorescent assay is 2.5uM, so it will not be suitable for detection of your 1uM samples. I am waiting for the lab to confirm compatibility with the reducing agen...

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Thank you for your inquiry. Unfortunately we have not specifically tested the compatibilities of these compounds with this kit, so we cannot say with certainty whether these will be compatible. The kit should provide up to 100 tests (in...

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Thank you for your follow up question. The lab producing this kit informed us that unfortunately the reaction mechanism information is proprietary and hence we cannot list all details except the one on the data sheet. Regarding the second...

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Thank you for your inquiry. Unfortunately we do not have any references for this product as yet. I would like however to reassure you that we do guarantee this product to work as stated on the datasheet. We do indeed guarantee all our products to w...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"