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Coenzyme A Detection Kit (ab102504)

Q&A (5)

Overview

  • Product nameCoenzyme A Detection KitSee all Coenzyme A kits ...
  • Tests
    100 x 1 assay
  • Sample type
    Urine, Serum, Plasma, Other biological fluids, Tissue Extracts, Cell Lysate, Cell culture media
  • Assay typeQuantitative
  • Sensitivity
    > 2.5 µM
  • Range
    2.5 µM - 250 µM
  • Assay time
    1h 00m
  • Product overview

    Abcam's Coenzyme A Detection Kit provides an easy, accurate assay to measure the CoA level in variety biological samples. In the assay, free CoA is specifically utilized to generate products which react with OxiRed Probe to generate color (λ = 570 nm) and fluorescence (Ex=535/Em=587 nm). The assay can detect 0.1 to 10 nmol of CoA (2.5-250 µM concentration range) in a variety of samples.
    Visit our FAQs page for tips and troubleshooting.

  • Notes

    Coenzyme A (CoA) is composed of units derived from cysteine, pantothenic acid, and ATP. It plays important roles in the synthesis and oxidation of fatty acids, pyruvate oxidation in the citric acid cycle and many other biological processes. One of the main functions of CoA is the carrying and transfer of acyl groups. One of the most important acyl groups transferred is the acetate group, in which case the molecule is called acetyl-CoA. The acetyl group eventually finds itself incorporated into a variety of molecules such as cholesterol, acetylcholine, melatonin, heme and the TCA cycle intermediates.

  • Tested applicationsFunctional Studies more details

Properties

  • RelevanceCoenzyme A (CoA) is composed of units derived from cysteine, pantothenic acid, and ATP. It plays important roles in the synthesis and oxidation of fatty acids, pyruvate oxidation in the citric acid cycle and many other biological processes. One of the main functions of Coenzyme A is the carrying and transfer of acyl groups. One of the most important acyl groups transferred is the acetate group, in which case the molecule is called acetyl-Coenzyme A. The acetyl group eventually finds itself incorporated into a variety of molecules such as cholesterol, acetylcholine, melatonin, heme and the TCA cycle intermediates.
    • Alternative names
        CoACoASHHSCoA

    Applications

    Our Abpromise guarantee covers the use of ab102504 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    Application Notes
    Functional Studies FuncS

    Coenzyme A Detection Kit images

    • Functional Studies - Coenzyme A Detection Kit (ab102504)
      Functional Studies - Coenzyme A Detection Kit (ab102504)
      Example of Standard Curve obtained using ab102504

    Protocols

    References for Coenzyme A Detection Kit (ab102504)

    ab102504 has not yet been referenced specifically in any publications.

    Publishing research using ab102504 ? Please let us know so that we can cite the reference in this datasheet

    Product Wall

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    Displaying 1 - 5 of 5 results for Abreviews and Q&A

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    I know it cannot detect 1uM, but can it detect a change of 1uM? For example, between 2.5uM and 3.5uM?

    The DTT concentration could be as high as 100uM, or BME at 1mM. For TCEP, I'd prefer 100uM, but could be more flexible in the amount.

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    Thank you for the information, and the clarification. I am not sure we have any data for precision, which would tell us how well the assay could distinguish among differences. The low standard, 0.2 nmol per well in the fluorescent version of the assay, is...

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    Will DTT, BME, or TCEP interfere with this assay? Also, I need to detect changes in CoA as low as 1uM (in a 75ul sample). Is this kit sensitive enough to detect that small of a change?

    Thank you for your interest in the Coenzyme A assay, ab102504. The detection limit of the fluorescent assay is 2.5uM, so it will not be suitable for detection of your 1uM samples. I am waiting for the lab to confirm compatibility with the reducing agents ...

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    I have lysates in MeOH, acetyl nitryl, and H2O (40:40:20). Is this solution compatible with the kit? How many samples + standards can be run with one kit?

    Thank you for your inquiry. Unfortunately we have not specifically tested the compatibilities of these compounds with this kit, so we cannot say with certainty whether these will be compatible. The kit should provide up to 100 tests (inclu...

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    Dear Sir/Madam,   There is something about the theory of the kit that I intented to know. As I understanded, the aim of the Coenzyme A Detection Kit is to convert CoA into Acyl-CoA, then detect the byproduct NADH to complete the assay. Is that ri...

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    Thank you for your follow up question. The lab producing this kit informed us that unfortunately the reaction mechanism information is proprietary and hence we cannot list all details except the one on the data sheet. Regarding the second pa...

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    Dear Sir/Madam,     My research team, which focuses on lipid metabolism, needs to measure Coenzyme A in our project. We noticed that this Coenzyme A Detection Kit (ab102504) may be very helpful.     So I am wondering could you offer some citations or re...

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    Thank you for your inquiry. Unfortunately we do not have any references for this product as yet. I would like however to reassure you that we do guarantee this product to work as stated on the datasheet. We do indeed guarantee all our products to work...

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"