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Anti-Cofilin antibody (ab11062)

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Overview

Product name

Anti-Cofilin antibody
See all Cofilin products (13) ...

Description

Rabbit polyclonal to Cofilin

Tested applications

WB, IF, ICC/IFmore details

Cross reactivity

Reacts with

Mouse, Rat, Chicken, Dog, Human, Pig

Immunogen

Synthetic peptide corresponding to amino acids 154-166 of human cofilin with N-terminal cysteine added, conjugated to maleimide-activated keyhole limpet hemocyanin (KLH). The corresponding immunizing sequence is identical in pig and rat non-muscle cofilin and differs by three amino acids from that of human and chicken muscle cofilin.

General notes

The corresponding immunizing sequence is identical in pig and rat non-muscle cofilin and differs by three amino acids from that of human and chicken muscle cofilin.

Properties

Form

Liquid

Storage instructions

Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.

Storage buffer

Preservative: 15mM Sodium Azide
Constituents: 0.01M PBS, pH 7.4

Concentration

Concentration information loading...

Purity

IgG fraction

Purification notes

Whole antiserum is fractionated and further purified by ion-exchange chromatography to provide the IgG fraction of antiserum that is essentially free of other rabbit serum proteins.

Clonality

Polyclonal

Isotype

IgG

Research areas

Cardiovascular >> Vasculature >> Smooth muscle cell (SMC) >> Cytoskeleton
Cardiovascular >> Heart >> Contractility >> Contractile Proteins >> Actins
Cancer >> Invasion/microenvironment >> ECM >> Cell adhesion >> Other
Neuroscience >> Cell Type Marker >> Neuron marker >> Axon marker
Neuroscience >> Neurology process >> Growth and Development >> Axonal Guidance Proteins
Signal Transduction >> Cytoskeleton / ECM >> Cytoskeleton >> Microfilaments >> Actin etc >> Actin Binding Proteins

  • Immunocytochemistry/ Immunofluorescence - Cofilin antibody (ab11062)Immunocytochemistry/ Immunofluorescence - Cofilin antibody (ab11062) image (enlarge)

Applications

Show applications key

Our Abpromise guarantee covers the use of ab11062 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application notes

ICC/IF: Use at an assay dependent dilution (PMID 19751773).
IF: 1/1000.
WB: 1/10000. Predicted molecular weight: 19 kDa.


Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.

Target

Function

Controls reversibly actin polymerization and depolymerization in a pH-sensitive manner. It has the ability to bind G- and F-actin in a 1:1 ratio of cofilin to actin. It is the major component of intranuclear and cytoplasmic actin rods.

Tissue specificity

Widely distributed in various tissues.

Sequence similarities

Belongs to the actin-binding proteins ADF family.
Contains 1 ADF-H domain.

Post-translational
modifications

Phosphorylated on Ser-3 in resting cells.

Cellular localization

Nucleus matrix. Cytoplasm > cytoskeleton. Almost completely in nucleus in cells exposed to heat shock or 10% dimethyl sulfoxide.

Target information above from: UniProt accessionP23528 The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).

Information by UniProt

Alternative names

  • non-muscle isoform antibody
  • 18 kDa phosphoprotein antibody
  • CFL 1 antibody
  • CFL antibody
  • CFL1 antibody
  • COF1_HUMAN antibody
  • Cofilin 1 antibody
  • Cofilin 1 non muscle antibody
  • Cofilin antibody
  • Cofilin non muscle isoform antibody
  • Cofilin-1 antibody
  • p18 antibody
see all

Database links

see all

Anti-Cofilin antibody images:

  Immunocytochemistry/ Immunofluorescence - Cofilin antibody (ab11062)

Immunocytochemistry/ Immunofluorescence - Cofilin antibody (ab11062)

ICC/IF image of ab11062 stained human HeLa cells. The cells were 4% PFA fixed (10 min) and then incubated in 1% BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab11062, 1 µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue). This antibody also gave a positive IF result in Hek293, HepG2 and MCF7 cells.

References for Anti-Cofilin antibody (ab11062)

This product has been referenced in:

  • Stoeckelhuber M  et al. Human axillary apocrine glands: proteins involved in the apocrine secretory mechanism. Histol Histopathol 26:177-84 (2011). IHC-P; Human.Read more (PubMed: 21154231) »
  • Truttmann MC  et al. Combined action of the type IV secretion effector proteins BepC and BepF promotes invasome formation of Bartonella henselae on endothelial and epithelial cells. Cell Microbiol 13:284-99 (2011). ICC/IF; Human.Read more (PubMed: 20964799) »

See all 8 publications for this product

Publishing research using ab11062? Please let us know so that we can cite the reference in this datasheet

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"