Anti-Collagen I + Collagen III antibody (ab24135)

Overview

• Product nameAnti-Collagen I + Collagen III antibodySee all Collagen I + Collagen III primary antibodies ...
• Description
  Rabbit polyclonal to Collagen I + Collagen III
• SpecificitySpecificity is measured by ELISA using pure pig collagen types I and III. No binding to pig serum proteins is seen at similar dilutions of the antibody. No cross reactivity seen against pig basement membrane collagens type IV and V in immunostaining.
• Tested applicationsDot Blot, ELISA, IHC-Fr more details
• Species reactivity
  Reacts with: Pig
  
• Immunogen

  Full length native collagen type I and III from pig skin.

• Positive controlPig kidney and skin. Cultured porcine cells.

Properties

• FormLyophilised:Reconstitute with 1ml of distilled water and add preservative if required.
• Storage instructionsStore at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze / thaw cycles.
• Storage bufferPreservative: None
  Constituents: Whole serum
• Concentration information loading...
• PurityImmunogen affinity purified
• Purification notesPooled antisera are passed over DEAE-cellulose to produce an IgG enriched fraction. Antibodies specific to type I and type III pig collagen are then immobilised by affinity chromatography and eluted with an acidic buffer. The final purification steps are neutralisation, dialysis and lyophilisation.
• Clonality Polyclonal
• IsotypeIgG
• Research Areas
  • Signal Transduction
  • Cytoskeleton / ECM
  • Extracellular Matrix
  • ECM Proteins
  • Collagen

Applications

Target

• RelevanceCollagen I is a fibrillar collagen found in most connective tissues, and the only component of the collagen found in cartilage. Mutations in this gene are associated with osteogenesis imperfecta, Ehlers Danlos syndrome, and idiopathic osteoporosis. Reciprocal translocations between chromosomes 17 and 22, where this gene and the gene for platelet derived growth factor beta are located, are associated with a particular type of skin tumor called dermatofibrosarcoma protuberans, resulting from unregulated expression of the growth factor. Two transcripts, resulting from the use of alternate polyadenylation signals, have been identified for this gene. Collagen III is a fibrillar collagen that is found in extensible connective tissues such as skin, lung, and the vascular system, frequently in association with Collagen I. Mutations in this gene are associated with Ehlers Danlos syndrome type IV, and with aortic and arterial aneurysms. Although alternate transcripts have been detected for this gene, they are the result of mutations; these mutations alter splicing, often leading to the exclusion of multiple exons.
• Cellular localizationSecreted

• Alternative names
  Alpha 1 type I collagen antibodyAlpha 2 type I collagen antibodyCOL1A1 antibody

  COL1A1 antibodyCOL1A2 antibodyCOL3A1 antibodyCollagen fetal antibodyCollagen type I alpha 1 antibodyCollagen type I alpha 2 antibodyCollagen type III alpha 1 antibodyEDS4A antibodyEhlers Danlos syndrome type IV autosomal dominant antibodyOI4 antibodyType I collagen alpha 1 chain antibodyType I procollagen antibody

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