Overview

  • Product name
    Anti-Collagen I + Collagen III antibody
    See all Collagen I + Collagen III primary antibodies
  • Description
    Rabbit polyclonal to Collagen I + Collagen III
  • Specificity
    Specificity was ascertained by direct ELISA using pure dog collagen types I and III for coating microplate wells. No binding to canine serum proteins is revealed at similar dilutions of the antibody.
  • Tested applications
    Suitable for: ELISA, WB, ICC, IHC-Frmore details
  • Species reactivity
    Reacts with: Dog
  • Immunogen

    Full length native protein (purified) (Dog). Extensively purified native collagen type I and III extracted from dog tail tendon.

  • Positive control
    • Native dog collagen type I and III for dot blotting and ELISA.

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • Storage buffer
    Preservative: None
    Constituents: The antibody was extracted into dilute acidic buffer after mild pepsin digestion.
  • Concentration information loading...
  • Purity
    IgG fraction
  • Purification notes
    Pooled antisera are passed over DEAE cellulose to produce IgG enriched fraction. The affinity purified antibody is obtained by binding to immobilized native dog collagen, mixture of type I and III (the antigens used for immunization), followed by elution with acidic buffer, neutralisation, dialysis, dispensing and lyophilization.
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab24137 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ELISA
WB
ICC
IHC-Fr
  • Application notes
    ELISA: Use at an assay dependent dilution.
    ICC: Use at an assay dependent dilution.
    IHC-Fr: Use at an assay dependent dilution. The antibody can be diluted at least 1/20 for immunohistochemical procedures if Peroxidase labeled secondary antibodies are applied. If a FITC labeled secondary antibody is used the antibody can be diluted 1/10.
    WB: Use at an assay dependent dilution.

    Not tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • Target

    • Relevance
      Collagen I is a fibrillar collagen found in most connective tissues, and the only component of the collagen found in cartilage. Mutations in this gene are associated with osteogenesis imperfecta, Ehlers Danlos syndrome, and idiopathic osteoporosis. Reciprocal translocations between chromosomes 17 and 22, where this gene and the gene for platelet derived growth factor beta are located, are associated with a particular type of skin tumor called dermatofibrosarcoma protuberans, resulting from unregulated expression of the growth factor. Two transcripts, resulting from the use of alternate polyadenylation signals, have been identified for this gene. Collagen III is a fibrillar collagen that is found in extensible connective tissues such as skin, lung, and the vascular system, frequently in association with Collagen I. Mutations in this gene are associated with Ehlers Danlos syndrome type IV, and with aortic and arterial aneurysms. Although alternate transcripts have been detected for this gene, they are the result of mutations; these mutations alter splicing, often leading to the exclusion of multiple exons.
    • Cellular localization
      Secreted
    • Alternative names
      • Alpha 1 type I collagen antibody
      • Alpha 2 type I collagen antibody
      • COL1A1 antibody
      • COL1A2 antibody
      • COL3A1 antibody
      • Collagen fetal antibody
      • Collagen type I alpha 1 antibody
      • Collagen type I alpha 2 antibody
      • Collagen type III alpha 1 antibody
      • OI4 antibody
      • Type I collagen alpha 1 chain antibody
      • Type I procollagen antibody
      see all

    References

    This product has been referenced in:
    • Sivasubramanian S  et al. Phenotypic Screening Identifies Synergistically Acting Natural Product Enhancing the Performance of Biomaterial Based Wound Healing. Front Pharmacol 8:433 (2017). WB . Read more (PubMed: 28769790) »
    • Chen WQ  et al. Variations of protein levels in human amniotic fluid stem cells CD117/2 over passages 5-25. J Proteome Res 8:5285-95 (2009). WB . Read more (PubMed: 19791749) »

    See all 2 Publications for this product

    Customer reviews and Q&As

    Application
    Western blot
    Sample
    Mouse Tissue lysate - whole (Hippocampus)
    Loading amount
    10 µg
    Specification
    Hippocampus
    Gel Running Conditions
    Reduced Denaturing
    Blocking step
    Milk as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
    Username

    Abcam user community

    Verified customer

    Submitted Aug 16 2010

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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