Anti-Collagen I antibody [5D8] (ab23446)

Overview

• Product nameAnti-Collagen I antibody [5D8]See all Collagen I primary antibodies ...
• Description
  Mouse monoclonal [5D8] to Collagen I
• SpecificityAb23446 is highly specific for type I collagen. The native triple-helical conformation is required for reaction. It has no cross reactivity with type II, III, V and VI collagens. There is no evidence for cross reactivity with other connnective tissue proteins (laminin, fibronectin, elastin).
• Tested applicationsELISA, ICC/IF, Flow Cyt, WB, IHC-Fr more details
• Species reactivity
  Reacts with: Sheep, Goat, Cow, Dog, Human, Pig
  

  Does not react with

  Mouse, Rabbit, Horse, Chicken, Guinea pig, Cat, Kangaroo
• Immunogen

  Full length native protein (purified): Acid digested pepsin soluble human type I collagen

• EpitopeThe epitope recognised by ab23446 is located near the C terminal of the molecule.
• Positive controlhuman and bovine skin

Properties

• FormLiquid
• Storage instructionsStore at +4°C in the dark.
• Storage bufferPreservative: 15mM Sodium Azide
  Constituents: 500mM Sodium chloride, 10mM PBS, pH 7.4
• Concentration information loading...
• PurityProtein G purified
• Clonality Monoclonal
• Clone number5D8
• MyelomaNS1/1-Ag4-1
• IsotypeIgG1
• Light chain typekappa
• Research Areas
  • Stem Cells
  • Mesenchymal Stem Cells
  • Osteogenesis

  • Signal Transduction
  • Cytoskeleton / ECM
  • Extracellular Matrix
  • ECM Proteins
  • Collagen

Applications

Target

• FunctionType I collagen is a member of group I collagen (fibrillar forming collagen).
• Tissue specificityForms the fibrils of tendon, ligaments and bones. In bones the fibrils are mineralized with calcium hydroxyapatite.
• Involvement in diseaseDefects in COL1A1 are the cause of Caffey disease (CAFFD) [MIM:114000]; also known as infantile cortical hyperostosis. Caffey disease is characterized by an infantile episode of massive subperiosteal new bone formation that typically involves the diaphyses of the long bones, mandible, and clavicles. The involved bones may also appear inflamed, with painful swelling and systemic fever often accompanying the illness. The bone changes usually begin before 5 months of age and resolve before 2 years of age.
  Defects in COL1A1 are a cause of Ehlers-Danlos syndrome type 1 (EDS1) [MIM:130000]; also known as Ehlers-Danlos syndrome gravis. EDS is a connective tissue disorder characterized by hyperextensible skin, atrophic cutaneous scars due to tissue fragility and joint hyperlaxity. EDS1 is the severe form of classic Ehlers-Danlos syndrome.
  Defects in COL1A1 are the cause of Ehlers-Danlos syndrome type 7A (EDS7A) [MIM:130060]; also known as autosomal dominant Ehlers-Danlos syndrome type VII. EDS is a connective tissue disorder characterized by hyperextensible skin, atrophic cutaneous scars due to tissue fragility and joint hyperlaxity. EDS7A is marked by bilateral congenital hip dislocation, hyperlaxity of the joints, and recurrent partial dislocations.
  Defects in COL1A1 are a cause of osteogenesis imperfecta type 1 (OI1) [MIM:166200]. A dominantly inherited connective tissue disorder characterized by bone fragility and blue sclerae. Osteogenesis imperfecta type 1 is non-deforming with normal height or mild short stature, and no dentinogenesis imperfecta.
  Defects in COL1A1 are a cause of osteogenesis imperfecta type 2A (OI2A) [MIM:166210]; also known as osteogenesis imperfecta congenita. A connective tissue disorder characterized by bone fragility, with many perinatal fractures, severe bowing of long bones, undermineralization, and death in the perinatal period due to respiratory insufficiency.
  Defects in COL1A1 are a cause of osteogenesis imperfecta type 3 (OI3) [MIM:259420]. A connective tissue disorder characterized by progressively deforming bones, very short stature, a triangular face, severe scoliosis, grayish sclera, and dentinogenesis imperfecta.
  Defects in COL1A1 are a cause of osteogenesis imperfecta type 4 (OI4) [MIM:166220]; also known as osteogenesis imperfecta with normal sclerae. A connective tissue disorder characterized by moderately short stature, mild to moderate scoliosis, grayish or white sclera and dentinogenesis imperfecta.
  Genetic variations in COL1A1 are a cause of susceptibility to osteoporosis (OSTEOP) [MIM:166710]; also known as involutional or senile osteoporosis or postmenopausal osteoporosis. Osteoporosis is characterized by reduced bone mass, disruption of bone microarchitecture without alteration in the composition of bone. Osteoporotic bones are more at risk of fracture.
  Note=A chromosomal aberration involving COL1A1 is found in dermatofibrosarcoma protuberans. Translocation t(17;22)(q22;q13) with PDGF.
• Sequence similaritiesBelongs to the fibrillar collagen family.
  Contains 1 fibrillar collagen NC1 domain.
  Contains 1 VWFC domain.
• Post-translational
  modificationsProline residues at the third position of the tripeptide repeating unit (G-X-Y) are hydroxylated in some or all of the chains. Proline residues at the second position of the tripeptide repeating unit (G-X-Y) are hydroxylated in some of the chains.
  O-linked glycan consists of a Glc-Gal disaccharide bound to the oxygen atom of a post-translationally added hydroxyl group.
• Cellular localizationSecreted > extracellular space > extracellular matrix.

Target information above from: UniProt accession P02452 The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010) .

Information by UniProt
• Database links
  • Entrez Gene: 282187 Cow
  • Entrez Gene: 282188 Cow
  • Entrez Gene: 403651 Dog
  • Entrez Gene: 403824 Dog
  • Entrez Gene: 1278 Human
  • Entrez Gene: 1277 Human
  • Omim: 120150 Human
  • Omim: 120160 Human

  • SwissProt: P02465 Cow
  • SwissProt: P02453 Cow
  • SwissProt: P08123 Human
  • SwissProt: P02452 Human
  • Unigene: 489142 Human
  • Unigene: 172928 Human
  • Unigene: 681002 Human

  see all

• Alternative names
  Alpha 1 type I collagen antibodyAlpha 2 type I collagen antibodyAlpha-1 type I collagen antibody

  CO1A1_HUMAN antibodyCOL1A1 antibodyCOL1A2 antibodyCollagen alpha-1(I) chain antibodyCollagen I alpha 1 polypeptide antibodyCollagen I alpha 2 polypeptide antibodyCollagen Of Skin Tendon And Bone antibodyCollagen Type 1 antibodyCollagen type I alpha 1 antibodyCollagen type I alpha 2 antibodyOI4 antibodyOsteogenesis Imperfecta Type IV antibodyPro alpha 1(I) collagen antibodyType I procollagen antibody

  see all