Anti-Collagen I antibody [5D8] (ab23446)
- Product nameAnti-Collagen I antibody [5D8]See all Collagen I primary antibodies ...
- DescriptionMouse monoclonal [5D8] to Collagen I
- SpecificityAb23446 is highly specific for type I collagen. The native triple-helical conformation is required for reaction. It has no cross reactivity with type II, III, V and VI collagens. There is no evidence for cross reactivity with other connnective tissue proteins (laminin, fibronectin, elastin).
- Tested applicationsELISA, ICC/IF, Flow Cyt, WB, IHC-Fr more details
- Species reactivityReacts with: Sheep, Goat, Cow, Dog, Human, Pig
Does not react withMouse, Rabbit, Horse, Chicken, Guinea pig, Cat, Kangaroo
Full length native protein (purified): Acid digested pepsin soluble human type I collagen
- EpitopeThe epitope recognised by ab23446 is located near the C terminal of the molecule.
- Positive control
- human and bovine skin
- Storage instructionsStore at +4°C short term (1-2 weeks). Store at -20°C or -80°C. Avoid freeze / thaw cycle.
- Storage bufferPreservative: 15mM Sodium Azide
Constituents: 500mM Sodium chloride, 10mM PBS, pH 7.4
- Concentration information loading...
- PurityProtein G purified
- Clonality Monoclonal
- Clone number5D8
- Light chain typekappa
- Research Areas
Our Abpromise guarantee covers the use of ab23446 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|ICC/IF||ICC/IF: Use at an assay dependent concentration. PubMed: 20161070|
|Flow Cyt||Flow Cyt: Use at an assay dependent concentration. PubMed: 20161070|
|WB||WB: Use at an assay dependent dilution. Predicted molecular weight: 134 kDa. Conditions need to be native to detect Collagen I with this monoclonal antibody.|
|IHC-Fr||IHC-Fr: Use at an assay dependent dilution. Note: If fixation of tissue is required, acetone or ethanol is recommended.|
- FunctionType I collagen is a member of group I collagen (fibrillar forming collagen).
- Tissue specificityForms the fibrils of tendon, ligaments and bones. In bones the fibrils are mineralized with calcium hydroxyapatite.
- Involvement in diseaseDefects in COL1A1 are the cause of Caffey disease (CAFFD) [MIM:114000]; also known as infantile cortical hyperostosis. Caffey disease is characterized by an infantile episode of massive subperiosteal new bone formation that typically involves the diaphyses of the long bones, mandible, and clavicles. The involved bones may also appear inflamed, with painful swelling and systemic fever often accompanying the illness. The bone changes usually begin before 5 months of age and resolve before 2 years of age.
Defects in COL1A1 are a cause of Ehlers-Danlos syndrome type 1 (EDS1) [MIM:130000]; also known as Ehlers-Danlos syndrome gravis. EDS is a connective tissue disorder characterized by hyperextensible skin, atrophic cutaneous scars due to tissue fragility and joint hyperlaxity. EDS1 is the severe form of classic Ehlers-Danlos syndrome.
Defects in COL1A1 are the cause of Ehlers-Danlos syndrome type 7A (EDS7A) [MIM:130060]; also known as autosomal dominant Ehlers-Danlos syndrome type VII. EDS is a connective tissue disorder characterized by hyperextensible skin, atrophic cutaneous scars due to tissue fragility and joint hyperlaxity. EDS7A is marked by bilateral congenital hip dislocation, hyperlaxity of the joints, and recurrent partial dislocations.
Defects in COL1A1 are a cause of osteogenesis imperfecta type 1 (OI1) [MIM:166200]. A dominantly inherited connective tissue disorder characterized by bone fragility and blue sclerae. Osteogenesis imperfecta type 1 is non-deforming with normal height or mild short stature, and no dentinogenesis imperfecta.
Defects in COL1A1 are a cause of osteogenesis imperfecta type 2A (OI2A) [MIM:166210]; also known as osteogenesis imperfecta congenita. A connective tissue disorder characterized by bone fragility, with many perinatal fractures, severe bowing of long bones, undermineralization, and death in the perinatal period due to respiratory insufficiency.
Defects in COL1A1 are a cause of osteogenesis imperfecta type 3 (OI3) [MIM:259420]. A connective tissue disorder characterized by progressively deforming bones, very short stature, a triangular face, severe scoliosis, grayish sclera, and dentinogenesis imperfecta.
Defects in COL1A1 are a cause of osteogenesis imperfecta type 4 (OI4) [MIM:166220]; also known as osteogenesis imperfecta with normal sclerae. A connective tissue disorder characterized by moderately short stature, mild to moderate scoliosis, grayish or white sclera and dentinogenesis imperfecta.
Genetic variations in COL1A1 are a cause of susceptibility to osteoporosis (OSTEOP) [MIM:166710]; also known as involutional or senile osteoporosis or postmenopausal osteoporosis. Osteoporosis is characterized by reduced bone mass, disruption of bone microarchitecture without alteration in the composition of bone. Osteoporotic bones are more at risk of fracture.
Note=A chromosomal aberration involving COL1A1 is found in dermatofibrosarcoma protuberans. Translocation t(17;22)(q22;q13) with PDGF.
- Sequence similaritiesBelongs to the fibrillar collagen family.
Contains 1 fibrillar collagen NC1 domain.
Contains 1 VWFC domain.
modificationsProline residues at the third position of the tripeptide repeating unit (G-X-Y) are hydroxylated in some or all of the chains. Proline residues at the second position of the tripeptide repeating unit (G-X-Y) are hydroxylated in some of the chains.
O-linked glycan consists of a Glc-Gal disaccharide bound to the oxygen atom of a post-translationally added hydroxyl group.
- Cellular localizationSecreted > extracellular space > extracellular matrix.
- Entrez Gene: 282187 Cow
- Entrez Gene: 282188 Cow
- Entrez Gene: 403651 Dog
- Entrez Gene: 403824 Dog
- Entrez Gene: 1277 Human
- Entrez Gene: 1278 Human
- Omim: 120150 Human
- Omim: 120160 Human
- SwissProt: P02453 Cow
- SwissProt: P02465 Cow
- SwissProt: P02452 Human
- SwissProt: P08123 Human
- Unigene: 172928 Human
- Unigene: 489142 Human
- Unigene: 681002 Human
- Alpha 1 type I collagen antibody
- Alpha 2 type I collagen antibody
- Alpha-1 type I collagen antibody
- CO1A1_HUMAN antibody
- COL1A1 antibody
- COL1A2 antibody
- Collagen alpha-1(I) chain antibody
- Collagen I alpha 1 polypeptide antibody
- Collagen I alpha 2 polypeptide antibody
- Collagen Of Skin Tendon And Bone antibody
- Collagen Type 1 antibody
- Collagen type I alpha 1 antibody
- Collagen type I alpha 2 antibody
- OI4 antibody
- Osteogenesis Imperfecta Type IV antibody
- Pro alpha 1(I) collagen antibody
- Type I procollagen antibody
References for Anti-Collagen I antibody [5D8] (ab23446)
This product has been referenced in:
- Jurgens WJ et al. One-step surgical procedure for the treatment of osteochondral defects with adipose-derived stem cells in a caprine knee defect: a pilot study. Biores Open Access 2:315-25 (2013). IHC ; Goat . Read more (PubMed: 23914338) »
- Flagler DJ et al. Intracellular Flow Cytometric Measurement of Extracellular Matrix Components in Porcine Intervertebral Disc Cells. Cell Mol Bioeng 2:264-273 (2009). Flow Cyt, ICC/IF ; Pig . Read more (PubMed: 20161070) »