Overview

  • Product nameAnti-Collagen I antibody (Biotin)See all Collagen I primary antibodies ...
  • Description
    Rabbit polyclonal to Collagen I (Biotin)
  • ConjugationBiotin
  • Conjugation notesBiotin
  • SpecificityNegligible cross-reactivity with Type II, III, IV, V or VI collagens. Non-specific cross reaction of anti-collagen antibodies with other human serum proteins or non-collagen extracellular matrix proteins is negligible.
  • Tested applicationsELISA, IHC-Fr, Immunomicroscopy, IP, WBmore details
  • Species reactivity
    Reacts with: Cow, Human
    Predicted to work with: all Mammals
  • Immunogen

    Collagen Type I from adult human knee cartilage and bovine nasal cartilage.

  • General notesAt least 11 genetically distinct gene products are collectively referred to as 'collagen types' or other proteins and proteoglycans of the extracellular matrix. In humans, collagens are composed of about 20 unique protein chains which under go various types of post-translational modifications and are ultimately assembled into a triple helix. This results in great diversity between collagen types. Collagens are highly conserved throughout evolution and are characterized by an uninterrupted "Glycine-X-Y" triplet repeat that is a necessary part of the triple helical structure. For these reasons it is often extremely difficult to generate antibodies with specificities to collagens. The development of type specific antibodies is dependent on NON-DENATURED three-dimensional epitopes. This preparation results in a native conformation of the protein.


    This antibody is well suited to detect extracellular matrix proteins in normal as well as disease state tissues. Disruption of tissue organization is the hallmark of neoplasia. Malignant lesions can be distinguished from benign by examining the breakdown of basement membranes and loss of 3-dimensional architecture. Malignant cells are presumed to use matrix metalloproteases to degrade barriers created by the extracellular matrix which then allows metastasis to occur. Collagenases, stomelysins and gelatinases can collectively degrade all of the various components of the extracellular matrix, including fibrillar and non-fibrillar collagens and basement membrane glycoproteins.

Properties

  • FormLiquid
  • Storage instructionsStore at +4°C short term (1-2 weeks). Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage bufferpH: 8
    Preservative: 0.01% Sodium azide
    Constituents: 0.44% Sodium chloride, 1% BSA, 0.15% EDTA, 4.8% Sodium borate
  • Concentration information loading...
  • PurityImmunogen affinity purified
  • Purification notesImmunoaffinity chromatography using immobilized antigens followed by extensive cross-adsorption against other collagens, human serum proteins and non-collagen extracellular matrix proteins to remove any unwanted specificities.
  • Primary antibody notes This antibody is well suited to detect extracellular matrix proteins in normal as well as disease state tissues. Disruption of tissue organization is the hallmark of neoplasia. Malignant lesions can be distinguished from benign by examining the breakdown of basement membranes and loss of 3-dimensional architecture. Malignant cells are presumed to use matrix metalloproteases to degrade barriers created by the extracellular matrix which then allows metastasis to occur. Collagenases, stomelysins and gelatinases can collectively degrade all of the various components of the extracellular matrix, including fibrillar and non-fibrillar collagens and basement membrane glycoproteins.
  • Clonality Polyclonal
  • IsotypeIgG
  • Research Areas

Applications

Our Abpromise guarantee covers the use of ab6577 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ELISA Use at an assay dependent dilution. 1/3000 - 1/6000.
IHC-Fr Use at an assay dependent dilution.
Immunomicroscopy Use at an assay dependent dilution.
IP Use at an assay dependent dilution. 1/100.
WB Use at an assay dependent dilution. 1/3000 - 1/6000.

Target

  • FunctionType I collagen is a member of group I collagen (fibrillar forming collagen).
  • Tissue specificityForms the fibrils of tendon, ligaments and bones. In bones the fibrils are mineralized with calcium hydroxyapatite.
  • Involvement in diseaseDefects in COL1A1 are the cause of Caffey disease (CAFFD) [MIM:114000]; also known as infantile cortical hyperostosis. Caffey disease is characterized by an infantile episode of massive subperiosteal new bone formation that typically involves the diaphyses of the long bones, mandible, and clavicles. The involved bones may also appear inflamed, with painful swelling and systemic fever often accompanying the illness. The bone changes usually begin before 5 months of age and resolve before 2 years of age.
    Defects in COL1A1 are a cause of Ehlers-Danlos syndrome type 1 (EDS1) [MIM:130000]; also known as Ehlers-Danlos syndrome gravis. EDS is a connective tissue disorder characterized by hyperextensible skin, atrophic cutaneous scars due to tissue fragility and joint hyperlaxity. EDS1 is the severe form of classic Ehlers-Danlos syndrome.
    Defects in COL1A1 are the cause of Ehlers-Danlos syndrome type 7A (EDS7A) [MIM:130060]; also known as autosomal dominant Ehlers-Danlos syndrome type VII. EDS is a connective tissue disorder characterized by hyperextensible skin, atrophic cutaneous scars due to tissue fragility and joint hyperlaxity. EDS7A is marked by bilateral congenital hip dislocation, hyperlaxity of the joints, and recurrent partial dislocations.
    Defects in COL1A1 are a cause of osteogenesis imperfecta type 1 (OI1) [MIM:166200]. A dominantly inherited connective tissue disorder characterized by bone fragility and blue sclerae. Osteogenesis imperfecta type 1 is non-deforming with normal height or mild short stature, and no dentinogenesis imperfecta.
    Defects in COL1A1 are a cause of osteogenesis imperfecta type 2A (OI2A) [MIM:166210]; also known as osteogenesis imperfecta congenita. A connective tissue disorder characterized by bone fragility, with many perinatal fractures, severe bowing of long bones, undermineralization, and death in the perinatal period due to respiratory insufficiency.
    Defects in COL1A1 are a cause of osteogenesis imperfecta type 3 (OI3) [MIM:259420]. A connective tissue disorder characterized by progressively deforming bones, very short stature, a triangular face, severe scoliosis, grayish sclera, and dentinogenesis imperfecta.
    Defects in COL1A1 are a cause of osteogenesis imperfecta type 4 (OI4) [MIM:166220]; also known as osteogenesis imperfecta with normal sclerae. A connective tissue disorder characterized by moderately short stature, mild to moderate scoliosis, grayish or white sclera and dentinogenesis imperfecta.
    Genetic variations in COL1A1 are a cause of susceptibility to osteoporosis (OSTEOP) [MIM:166710]; also known as involutional or senile osteoporosis or postmenopausal osteoporosis. Osteoporosis is characterized by reduced bone mass, disruption of bone microarchitecture without alteration in the composition of bone. Osteoporotic bones are more at risk of fracture.
    Note=A chromosomal aberration involving COL1A1 is found in dermatofibrosarcoma protuberans. Translocation t(17;22)(q22;q13) with PDGF.
  • Sequence similaritiesBelongs to the fibrillar collagen family.
    Contains 1 fibrillar collagen NC1 domain.
    Contains 1 VWFC domain.
  • Post-translational
    modifications
    Proline residues at the third position of the tripeptide repeating unit (G-X-Y) are hydroxylated in some or all of the chains. Proline residues at the second position of the tripeptide repeating unit (G-X-Y) are hydroxylated in some of the chains.
    O-linked glycan consists of a Glc-Gal disaccharide bound to the oxygen atom of a post-translationally added hydroxyl group.
  • Cellular localizationSecreted > extracellular space > extracellular matrix.
  • Information by UniProt
  • Database links
  • Alternative names
    • Alpha 1 type I collagen antibody
    • Alpha 2 type I collagen antibody
    • Alpha-1 type I collagen antibody
    • CO1A1_HUMAN antibody
    • COL1A1 antibody
    • COL1A2 antibody
    • Collagen alpha-1(I) chain antibody
    • Collagen I alpha 1 polypeptide antibody
    • Collagen I alpha 2 polypeptide antibody
    • Collagen Of Skin Tendon And Bone antibody
    • Collagen Type 1 antibody
    • Collagen type I alpha 1 antibody
    • Collagen type I alpha 2 antibody
    • OI4 antibody
    • Osteogenesis Imperfecta Type IV antibody
    • Pro alpha 1(I) collagen antibody
    • Type I procollagen antibody
    see all

References for Anti-Collagen I antibody (Biotin) (ab6577)

This product has been referenced in:
  • Wen L  et al. Mild Electrical Pulse Current Stimulation Upregulates S100A4 and Promotes Cardiogenesis in MSC and Cardiac Myocytes Coculture Monolayer. Cell Biochem Biophys 65:43-55 (2013). Read more (PubMed: 22941361) »
  • Maghsoudlou P  et al. Preservation of micro-architecture and angiogenic potential in a pulmonary acellular matrix obtained using intermittent intra-tracheal flow of detergent enzymatic treatment. Biomaterials 34:6638-48 (2013). Read more (PubMed: 23727263) »

See all 3 Publications for this product

Product Wall

Thank you for your enquiry. You are correct, this ab34710 probably will not react well with your aged and denatured samples. This antibody will only bind with the intact 3D protein, so no denaturants can be used in the gel or in the buffers. I hope...

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Thank you for your email. What did your sample buffer consist of? To use ab6577 in Western blotting, you must use non-denaturing, non-dissociating Western blotting conditions. Please follow the suggestions below in order to use this antibody in Western...

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Please reference the book: Gel Electrophoresis of Proteins, A Practical Approach, edited by B.D. Hames and D. Rickwood.

This antibody has been shown to react with collagen type 1 in human and bovine using immunohistochemistry on frozen tissue sections.

The epitope recognized by ab8308 is sensitive to routine formalin fixation and paraffin embedding. Strong staining of connective tissue fibres is seen in acetone-fixed or unfixed frozen sections. So yes, this antibody has been tested with PES but has b...

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Yes, ab6577 is a biotinylated version of ab292

This antibody should work with ethanol or acetone in frozen tissue.

Anti-collagen should react with mouse and rat.

We use pepsin digestion and salt precipitation, not peptides as antigens for our anti-collagens. We do not have any information concerning the nature of the epitope that the antibody is recognising. We believe that these antibodies have not yet been...

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Sorry, they have not been tested in immunohistochemistry on mouse tissue.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"