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Full length native protein (purified) corresponding to Cow Collagen I.
Database link: P02452
IHC-P application: We have received positive as well as negative customer feedback for IHC-P. The antibody is not batch-tested in this application, thus additional troubleshooting might be needed to obtain satisfactory results.
Our Abpromise guarantee covers the use of ab90395 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IHC||Use at an assay dependent concentration. ab170190-Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.|
|Dot Blot||Use at an assay dependent concentration.
|IHC-Glut||1/100 - 1/800.
Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
|IHC-R||Use at an assay dependent concentration.|
|Indirect ELISA||Use at an assay dependent concentration.|
|WB||1/500 - 1/3000. Use under non reducing condition. Predicted molecular weight: 139 kDa.
Use native (non-denaturing) conditions, as the antibody does not recognise denatured protein.
|ELISA||Use at an assay dependent concentration.
|Electron Microscopy||1/100 - 1/1000. PubMed: 17016762for Gold labeling|
|IHC-FoFr||1/100. PubMed: 17016762Fix in Zamboni's solution (2% paraformaldehyde, 0.2% picric acid in phosphate-buffered saline (PBS), pH 7.6) for 2 h at 4C, store in 20% sucrose in 0.5 mM PBS at 4C.|
|IHC-P||Use at an assay dependent concentration. Perform enzymatic antigen retrieval before commencing with IHC staining protocol.|
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