Overview

  • Product nameAnti-Collagen I antibody [COL-1]
    See all Collagen I primary antibodies
  • Description
    Mouse monoclonal [COL-1] to Collagen I
  • Specificityab90395 will not cross react with collagen 2-11, or thermally denatured collagen. The antibody is reactive with the native (non-denaturing, helical) form of collagen type I and not reactive with hen tested on thermally denatured molecules. Use native (non-denaturing) conditions.
  • Tested applicationsDot Blot, IHC-Glut, IHC-R, IP, Indirect ELISA, WB, ELISA, IHC-Fr, ICC/IF, Electron Microscopy, IHC-FoFrmore details
  • Species reactivity
    Reacts with: Rat, Rabbit, Cow, Human, Pig, Deer
  • Immunogen

    Full length native protein (purified) corresponding to Cow Collagen I.
    Database link: P02452

  • Positive control
    • Connective tissue fibres. Pig skin tissue.
  • General notes

    IHC-P application: We have received positive as well as negative customer feedback for IHC-P. The antibody is not batch-tested in this application, thus additional troubleshooting might be needed to obtain satisfactory results.

Properties

Applications

Our Abpromise guarantee covers the use of ab90395 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Dot Blot Use at an assay dependent concentration.

Assay dependent

IHC-Glut Use at an assay dependent concentration.
IHC-R Use at an assay dependent concentration.
IP 1/50.
Indirect ELISA Use at an assay dependent concentration.
WB 1/500 - 1/3000. Use under non reducing condition. Predicted molecular weight: 139 kDa.

The antibody is reactive with the native (non-denaturing, helical) form of collagen type I and not reactive when tested on thermally denatured molecules. Use native (non-denaturing) conditions, as the antibody does not recognise denatured protein.

ELISA Use at an assay dependent concentration.

Assay dependent

IHC-Fr 1/2000.
ICC/IF 1/2000.
Electron Microscopy 1/100 - 1/1000. PubMed: 17016762for Gold labeling
IHC-FoFr 1/100. PubMed: 17016762Fix in Zamboni's solution (2% paraformaldehyde, 0.2% picric acid in phosphate-buffered saline (PBS), pH 7.6) for 2 h at 4C, store in 20% sucrose in 0.5 mM PBS at 4C.
  • Application notesIs unsuitable for IHC-P.
  • Target

    • FunctionType I collagen is a member of group I collagen (fibrillar forming collagen).
    • Tissue specificityForms the fibrils of tendon, ligaments and bones. In bones the fibrils are mineralized with calcium hydroxyapatite.
    • Involvement in diseaseDefects in COL1A1 are the cause of Caffey disease (CAFFD) [MIM:114000]; also known as infantile cortical hyperostosis. Caffey disease is characterized by an infantile episode of massive subperiosteal new bone formation that typically involves the diaphyses of the long bones, mandible, and clavicles. The involved bones may also appear inflamed, with painful swelling and systemic fever often accompanying the illness. The bone changes usually begin before 5 months of age and resolve before 2 years of age.
      Defects in COL1A1 are a cause of Ehlers-Danlos syndrome type 1 (EDS1) [MIM:130000]; also known as Ehlers-Danlos syndrome gravis. EDS is a connective tissue disorder characterized by hyperextensible skin, atrophic cutaneous scars due to tissue fragility and joint hyperlaxity. EDS1 is the severe form of classic Ehlers-Danlos syndrome.
      Defects in COL1A1 are the cause of Ehlers-Danlos syndrome type 7A (EDS7A) [MIM:130060]; also known as autosomal dominant Ehlers-Danlos syndrome type VII. EDS is a connective tissue disorder characterized by hyperextensible skin, atrophic cutaneous scars due to tissue fragility and joint hyperlaxity. EDS7A is marked by bilateral congenital hip dislocation, hyperlaxity of the joints, and recurrent partial dislocations.
      Defects in COL1A1 are a cause of osteogenesis imperfecta type 1 (OI1) [MIM:166200]. A dominantly inherited connective tissue disorder characterized by bone fragility and blue sclerae. Osteogenesis imperfecta type 1 is non-deforming with normal height or mild short stature, and no dentinogenesis imperfecta.
      Defects in COL1A1 are a cause of osteogenesis imperfecta type 2A (OI2A) [MIM:166210]; also known as osteogenesis imperfecta congenita. A connective tissue disorder characterized by bone fragility, with many perinatal fractures, severe bowing of long bones, undermineralization, and death in the perinatal period due to respiratory insufficiency.
      Defects in COL1A1 are a cause of osteogenesis imperfecta type 3 (OI3) [MIM:259420]. A connective tissue disorder characterized by progressively deforming bones, very short stature, a triangular face, severe scoliosis, grayish sclera, and dentinogenesis imperfecta.
      Defects in COL1A1 are a cause of osteogenesis imperfecta type 4 (OI4) [MIM:166220]; also known as osteogenesis imperfecta with normal sclerae. A connective tissue disorder characterized by moderately short stature, mild to moderate scoliosis, grayish or white sclera and dentinogenesis imperfecta.
      Genetic variations in COL1A1 are a cause of susceptibility to osteoporosis (OSTEOP) [MIM:166710]; also known as involutional or senile osteoporosis or postmenopausal osteoporosis. Osteoporosis is characterized by reduced bone mass, disruption of bone microarchitecture without alteration in the composition of bone. Osteoporotic bones are more at risk of fracture.
      Note=A chromosomal aberration involving COL1A1 is found in dermatofibrosarcoma protuberans. Translocation t(17;22)(q22;q13) with PDGF.
    • Sequence similaritiesBelongs to the fibrillar collagen family.
      Contains 1 fibrillar collagen NC1 domain.
      Contains 1 VWFC domain.
    • Post-translational
      modifications
      Proline residues at the third position of the tripeptide repeating unit (G-X-Y) are hydroxylated in some or all of the chains. Proline residues at the second position of the tripeptide repeating unit (G-X-Y) are hydroxylated in some of the chains.
      O-linked glycan consists of a Glc-Gal disaccharide bound to the oxygen atom of a post-translationally added hydroxyl group.
    • Cellular localizationSecreted > extracellular space > extracellular matrix.
    • Information by UniProt
    • Database links
    • Alternative names
      • Alpha 1 type I collagen antibody
      • Alpha 2 type I collagen antibody
      • alpha 2 type I procollagen antibody
      • alpha 2(I) procollagen antibody
      • alpha 2(I)-collagen antibody
      • Alpha-1 type I collagen antibody
      • alpha1(I) procollagen antibody
      • CO1A1_HUMAN antibody
      • COL1A1 antibody
      • COL1A2 antibody
      • collagen alpha 1 chain type I antibody
      • Collagen alpha-1(I) chain antibody
      • collagen alpha-1(I) chain preproprotein antibody
      • Collagen I alpha 1 polypeptide antibody
      • Collagen I alpha 2 polypeptide antibody
      • collagen of skin, tendon and bone, alpha-1 chain antibody
      • collagen of skin, tendon and bone, alpha-2 chain antibody
      • Collagen type I alpha 1 antibody
      • Collagen type I alpha 2 antibody
      • EDSC antibody
      • OI1 antibody
      • OI2 antibody
      • OI3 antibody
      • OI4 antibody
      • pro-alpha-1 collagen type 1 antibody
      • type I proalpha 1 antibody
      • type I procollagen alpha 1 chain antibody
      • Type I procollagen antibody
      see all

    Anti-Collagen I antibody [COL-1] images

    • ab90395 staining Collagen I in Pig Ureter tissue sections by Immunohistochemistry (IHC-Fr - frozen sections). Tissue was fixed with paraformaldehyde and blocked with 5% BSA for 30 minutes at 20°C. Samples were incubated with primary antibody (1/2000) for 4 hours at 2°C. A Cy2®-conjugated Goat anti-mouse polyclonal (1/800) was used as the secondary antibody.

      See Abreview

    • All lanes : Anti-Collagen I antibody [COL-1] (ab90395) at 1/1000 dilution

      Lane 1 : Recombinant Human Collagen I at 3 µg
      Lane 2 : Pig skin whole cell lysate extracted in Laemmli buffer at 20 µg

      Secondary
      HRP-conjugated Goat anti-mouse monoclonal IgG at 1/3000 dilution
      developed using the ECL technique

      Performed under non-reducing conditions.

      Predicted band size : 139 kDa
      Observed band size : 130,140 kDa (why is the actual band size different from the predicted?)
      Additional bands at : 250 kDa (possible dimer),400 kDa (possible multimer).

      Exposure time : 30 seconds

      This image is courtesy of an anonymous Abreview

      Blocked with 5% Milk for 1 hour at 20°C.

      See Abreview

    • ab90395 staining Collagen I in Pig skin tissue sections by Immunohistochemistry (IHC-Fr - frozen sections). Tissue was fixed with formaldehyde and blocked with 5% serum for 1 hour at 20°C. Samples were incubated with primary antibody (1/500 in PBS, Tween 0.01% + Donkey serum 1%) for 16 hours at 4°C. A Cy2® -conjugated Donkey anti-mouse IgG polyclonal (1/100) was used as the secondary antibody.

      See Abreview

    References for Anti-Collagen I antibody [COL-1] (ab90395)

    This product has been referenced in:
    • Eltawil NM  et al. The use of hyperosmotic saline for chondroprotection: implications for orthopaedic surgery and cartilage repair. Osteoarthritis Cartilage 23:469-77 (2015). Read more (PubMed: 25497864) »
    • Mia MM  et al. Interleukin-1ß attenuates myofibroblast formation and extracellular matrix production in dermal and lung fibroblasts exposed to transforming growth factor-ß1. PLoS One 9:e91559 (2014). ICC/IF ; Human . Read more (PubMed: 24622053) »

    See all 13 Publications for this product

    Product Wall

    Thank you for taking the time to complete our questionnaire and contact us. I am sorry to hear you have had difficulty obtaining satisfactory results from these antibodies.

    The details you have kindly provided will enable us to investigate t...

    Read More
    Abcam guarantees this product to work in the species/application used in this Abreview.
    Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Sample Human Tissue sections (muscle)
    Antigen retrieval step Heat mediated - Buffer/Enzyme Used: citrate buffer
    Specification muscle
    Blocking step (agent) for 1 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: 20°C
    Fixative Paraformaldehyde
    Username

    Abcam user community

    Verified customer

    Submitted Mar 16 2016

    Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Blocking step Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 37°C
    Antigen retrieval step Heat mediated - Buffer/Enzyme Used: 0.05% typsin
    Sample Human Tissue sections (human whole fat tissue)
    Specification human whole fat tissue
    Permeabilization No
    Fixative Formaldehyde
    Username

    Abcam user community

    Verified customer

    Submitted Mar 13 2015

    Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Blocking step BSA as blocking agent for 10 minute(s) · Concentration: 10% · Temperature: 25°C
    Antigen retrieval step Heat mediated - Buffer/Enzyme Used: sodium citrate buffer at pH=6.05
    Sample Sheep Tissue sections (tendon)
    Specification tendon
    Permeabilization No
    Fixative Formaldehyde
    Username

    Abcam user community

    Verified customer

    Submitted Dec 13 2013

    Abcam guarantees this product to work in the species/application used in this Abreview.
    Application Immunohistochemistry (Frozen sections)
    Sample Pig Tissue sections (Ureter from Minipig)
    Specification Ureter from Minipig
    Fixative Paraformaldehyde
    Permeabilization No
    Blocking step BSA as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 20°C
    Username

    Ms. Beate Thode

    Verified customer

    Submitted Feb 20 2013

    Abcam guarantees this product to work in the species/application used in this Abreview.
    Application Immunohistochemistry (Frozen sections)
    Sample Human Tissue sections (Ureter)
    Specification Ureter
    Fixative Paraformaldehyde
    Permeabilization No
    Blocking step BSA as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 20°C
    Username

    Ms. Beate Thode

    Verified customer

    Submitted Feb 19 2013

    Thank you very much for contacting us with your question.

    We don't have any data specifically against pro-collagen (recombinant testing in WB, for example), but since mature collagen is created by cleavage of the pro-collagen, I would expect ...

    Read More



    ab90395 is in form of ascites. The concentration is not determined.

    Thank you for contacting us.

    I have looked at the datasheet and indeed the information is confusing.

    Reducing and non-reducing conditions refers to whether or not there are reagents present that disrupt disulfide bonds between cyst...

    Read More

    Thank you very much for contacting us with your enquiry.

    The AMELX protein ab139212 can be diluted in any buffer. However, all of our products are intended for research use only and are not recommended for in vitro studies. This protein conta...

    Read More

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"