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Full length native protein (purified) corresponding to Cow Collagen I.
Database link: P02452
IHC-P application: We have received positive as well as negative customer feedback for IHC-P. The antibody is not batch-tested in this application, thus additional troubleshooting might be needed to obtain satisfactory results.
Our Abpromise guarantee covers the use of ab90395 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|Dot Blot||Use at an assay dependent concentration.
|IHC-R||Use at an assay dependent concentration.|
|Indirect ELISA||Use at an assay dependent concentration.|
|WB||1/500 - 1/3000. Use under non reducing condition. Predicted molecular weight: 139 kDa.
The antibody is reactive with the native (non-denaturing, helical) form of collagen type I and not reactive when tested on thermally denatured molecules. Use native (non-denaturing) conditions, as the antibody does not recognise denatured protein.
|ELISA||Use at an assay dependent concentration.
|Electron Microscopy||1/100 - 1/1000. PubMed: 17016762for Gold labeling|
|IHC-FoFr||1/100. PubMed: 17016762Fix in Zamboni's solution (2% paraformaldehyde, 0.2% picric acid in phosphate-buffered saline (PBS), pH 7.6) for 2 h at 4C, store in 20% sucrose in 0.5 mM PBS at 4C.|
|IHC-P||Use at an assay dependent concentration. Perform enzymatic antigen retrieval before commencing with IHC staining protocol.|
ab90395 staining Collagen I in Human muscle tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with paraformaldehyde and blocked with 1% Caseine for 1 hour at 20°C; antigen retrieval was by heat mediation in a citrate buffer. Samples were incubated with primary antibody (1/100 in PBS Casein 1%) for 1 hour at 20°C. An undiluted Biotin-conjugated Mouse polyclonal was used as the secondary antibody.
ab90395 staining Collagen I in Pig Ureter tissue sections by Immunohistochemistry (IHC-Fr - frozen sections). Tissue was fixed with paraformaldehyde and blocked with 5% BSA for 30 minutes at 20°C. Samples were incubated with primary antibody (1/2000) for 4 hours at 2°C. A Cy2®-conjugated Goat anti-mouse polyclonal (1/800) was used as the secondary antibody.
This image is courtesy of an anonymous AbreviewBlocked with 5% Milk for 1 hour at 20°C.
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