Anti-Collagen I antibody [COL-1] (ab90395)
- Product nameAnti-Collagen I antibody [COL-1]See all Collagen I primary antibodies ...
- DescriptionMouse monoclonal [COL-1] to Collagen I
- Specificityab90395 will not cross react with collagen 2-11, or thermally denatured collagen.
- Tested applicationsDot Blot, IHC-Glut, IHC-R, IP, Indirect ELISA, WB, ELISA, IHC-Fr, ICC/IF, Electron Microscopy, IHC-FoFr, IHC-P more details
- Species reactivityReacts with: Rat, Rabbit, Cow, Human, Pig, Deer
Full length native protein purified from cow
- Positive control
- Connective tissue fibres
- Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles.
- Storage bufferPreservative: None
Constituents: Ascites, PBS
- Concentration information loading...
- Clonality Monoclonal
- Clone numberCOL-1
- Research Areas
Our Abpromise guarantee covers the use of ab90395 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|Dot Blot||Dot: Use at an assay dependent dilution.|
|IHC-Glut||IHC-Glut: 1/100 - 1/800. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.|
|IHC-R||IHC-R: Use at an assay dependent dilution.|
|Indirect ELISA||I-ELISA: Use at an assay dependent dilution.|
|WB||WB: 1/500 - 1/3000. Use under non reducing condition. Detects a band of approximately 130 kDa (predicted molecular weight: 130 kDa). Use native (non-denaturing) conditions, as the antibody does not recognise denatured protein.|
|ELISA||ELISA: Use at an assay dependent dilution.|
|IHC-Fr||IHC-Fr: Use at an assay dependent dilution.|
|Electron Microscopy||EM: 1/100 - 1/1000. PubMed: 17016762for Gold labeling|
|IHC-FoFr||IHC-FoFr: 1/100. PubMed: 17016762Fix in Zamboni's solution (2% paraformaldehyde, 0.2% picric acid in phosphate-buffered saline (PBS), pH 7.6) for 2 h at 4C, store in 20% sucrose in 0.5 mM PBS at 4C.|
|IHC-P||IHC-P: Use at an assay dependent dilution. Perform enzymatic antigen retrieval before commencing with IHC staining protocol.|
- FunctionType I collagen is a member of group I collagen (fibrillar forming collagen).
- Tissue specificityForms the fibrils of tendon, ligaments and bones. In bones the fibrils are mineralized with calcium hydroxyapatite.
- Involvement in diseaseDefects in COL1A1 are the cause of Caffey disease (CAFFD) [MIM:114000]; also known as infantile cortical hyperostosis. Caffey disease is characterized by an infantile episode of massive subperiosteal new bone formation that typically involves the diaphyses of the long bones, mandible, and clavicles. The involved bones may also appear inflamed, with painful swelling and systemic fever often accompanying the illness. The bone changes usually begin before 5 months of age and resolve before 2 years of age.
Defects in COL1A1 are a cause of Ehlers-Danlos syndrome type 1 (EDS1) [MIM:130000]; also known as Ehlers-Danlos syndrome gravis. EDS is a connective tissue disorder characterized by hyperextensible skin, atrophic cutaneous scars due to tissue fragility and joint hyperlaxity. EDS1 is the severe form of classic Ehlers-Danlos syndrome.
Defects in COL1A1 are the cause of Ehlers-Danlos syndrome type 7A (EDS7A) [MIM:130060]; also known as autosomal dominant Ehlers-Danlos syndrome type VII. EDS is a connective tissue disorder characterized by hyperextensible skin, atrophic cutaneous scars due to tissue fragility and joint hyperlaxity. EDS7A is marked by bilateral congenital hip dislocation, hyperlaxity of the joints, and recurrent partial dislocations.
Defects in COL1A1 are a cause of osteogenesis imperfecta type 1 (OI1) [MIM:166200]. A dominantly inherited connective tissue disorder characterized by bone fragility and blue sclerae. Osteogenesis imperfecta type 1 is non-deforming with normal height or mild short stature, and no dentinogenesis imperfecta.
Defects in COL1A1 are a cause of osteogenesis imperfecta type 2A (OI2A) [MIM:166210]; also known as osteogenesis imperfecta congenita. A connective tissue disorder characterized by bone fragility, with many perinatal fractures, severe bowing of long bones, undermineralization, and death in the perinatal period due to respiratory insufficiency.
Defects in COL1A1 are a cause of osteogenesis imperfecta type 3 (OI3) [MIM:259420]. A connective tissue disorder characterized by progressively deforming bones, very short stature, a triangular face, severe scoliosis, grayish sclera, and dentinogenesis imperfecta.
Defects in COL1A1 are a cause of osteogenesis imperfecta type 4 (OI4) [MIM:166220]; also known as osteogenesis imperfecta with normal sclerae. A connective tissue disorder characterized by moderately short stature, mild to moderate scoliosis, grayish or white sclera and dentinogenesis imperfecta.
Genetic variations in COL1A1 are a cause of susceptibility to osteoporosis (OSTEOP) [MIM:166710]; also known as involutional or senile osteoporosis or postmenopausal osteoporosis. Osteoporosis is characterized by reduced bone mass, disruption of bone microarchitecture without alteration in the composition of bone. Osteoporotic bones are more at risk of fracture.
Note=A chromosomal aberration involving COL1A1 is found in dermatofibrosarcoma protuberans. Translocation t(17;22)(q22;q13) with PDGF.
- Sequence similaritiesBelongs to the fibrillar collagen family.
Contains 1 fibrillar collagen NC1 domain.
Contains 1 VWFC domain.
modificationsProline residues at the third position of the tripeptide repeating unit (G-X-Y) are hydroxylated in some or all of the chains. Proline residues at the second position of the tripeptide repeating unit (G-X-Y) are hydroxylated in some of the chains.
O-linked glycan consists of a Glc-Gal disaccharide bound to the oxygen atom of a post-translationally added hydroxyl group.
- Cellular localizationSecreted > extracellular space > extracellular matrix.
- Entrez Gene: 282187 Cow
- Entrez Gene: 282188 Cow
- Entrez Gene: 1277 Human
- Entrez Gene: 1278 Human
- Entrez Gene: 29393 Rat
- Entrez Gene: 84352 Rat
- Omim: 120150 Human
- Omim: 120160 Human
- SwissProt: P02453 Cow
- SwissProt: P02465 Cow
- SwissProt: P02452 Human
- SwissProt: P08123 Human
- SwissProt: P02454 Rat
- SwissProt: P02466 Rat
- Unigene: 172928 Human
- Unigene: 489142 Human
- Unigene: 681002 Human
- Unigene: 107239 Rat
- Unigene: 2953 Rat
- Alpha 1 type I collagen antibody
- Alpha 2 type I collagen antibody
- Alpha-1 type I collagen antibody
- CO1A1_HUMAN antibody
- COL1A1 antibody
- COL1A2 antibody
- Collagen alpha-1(I) chain antibody
- Collagen I alpha 1 polypeptide antibody
- Collagen I alpha 2 polypeptide antibody
- Collagen type I alpha 1 antibody
- Collagen type I alpha 2 antibody
- Type I procollagen antibody
Anti-Collagen I antibody [COL-1] images
ab90395 staining Collagen I in porcine ligament by Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections).
Tissue was fixed in paraformaldehyde and an enzymatic antigen retrieval step was performed. Samples were then blocked using 1% BSA for 30 minutes at 20°C, then incubated with ab90395 at a 1/400 dilution for 1 hour at 20°C. The secondary used was an anti-mouse polyclonal conjugated to biotin-maleimide at a 1/200 dilution.
ab90395 staining Collagen I in Pig skin tissue sections by Immunohistochemistry (IHC-Fr - frozen sections). Tissue was fixed with formaldehyde and blocked with 5% serum for 1 hour at 20°C. Samples were incubated with primary antibody (1/500 in PBS, Tween 0.01% + Donkey serum 1%) for 16 hours at 4°C. A Cy2® -conjugated Donkey anti-mouse IgG polyclonal (1/100) was used as the secondary antibody.
All lanes : Anti-Collagen I antibody [COL-1] (ab90395) at 1/1000 dilution
Lane 1 : Recombinant Human Collagen I at 3 µg
Lane 2 : Pig skin whole cell lysate extracted in Laemmli buffer at 20 µg
HRP-conjugated Goat anti-mouse monoclonal IgG at 1/3000 dilution
developed using the ECL technique
Performed under non-reducing conditions.
Predicted band size : 130 kDa
Observed band size : 130,140 kDa (why is the actual band size different from the predicted?)
Additional bands at : 250 kDa (possible dimer),400 kDa (possible multimer).
Exposure time : 30 seconds
This image is courtesy of an anonymous Abreview
References for Anti-Collagen I antibody [COL-1] (ab90395)
This product has been referenced in:
- Zong ming W et al. Bone formation in rabbit cancellous bone explant culture model is enhanced by mechanical load. Biomed Eng Online 12:35 (2013). WB ; Rabbit . Read more (PubMed: 23597232) »
- Song Z et al. Repair of abdominal wall defects in vitro and in vivo using VEGF sustained-release multi-walled carbon nanotubes (MWNT) composite scaffolds. PLoS One 8:e64358 (2013). IHC-P ; Rat . Read more (PubMed: 23717603) »