Overview

  • Product nameAnti-Collagen I antibodySee all Collagen I primary antibodies ...
  • Description
    Rabbit polyclonal to Collagen I
  • SpecificityCross-reactivity (% @ RIA 1:200 dilution): Mouse Collagen Type I 100%, Mouse Collagen Type III <1.0%, Mouse Collagen Type II, IV <0.1%. Human, Chicken, Rat Collagen Type I <0.1%
  • Tested applicationsFlow Cyt, IHC-Fr, RIA, ELISA, WB, ICC/IF, IHC-P more details
  • Species reactivity
    Reacts with: Mouse
  • Immunogen

    Collagen type I extracted and purified from mouse skin.

  • Positive control
    • Frozen mouse liver for IF.

Properties

Applications

Our Abpromise guarantee covers the use of ab21286 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Notes
Flow Cyt Use at an assay dependent concentration.
IHC-Fr 1/40. Tested using a FITC-labeled anti-rabbit IgG secondary.
RIA 1/200.
ELISA Use at an assay dependent concentration.
WB Use at an assay dependent concentration.
ICC/IF Use at an assay dependent concentration.
IHC-P 1/250. Tested using an HRP-labeled anti-rabbit IgG secondary.

Target

  • FunctionType I collagen is a member of group I collagen (fibrillar forming collagen).
  • Tissue specificityForms the fibrils of tendon, ligaments and bones. In bones the fibrils are mineralized with calcium hydroxyapatite.
  • Involvement in diseaseDefects in COL1A1 are the cause of Caffey disease (CAFFD) [MIM:114000]; also known as infantile cortical hyperostosis. Caffey disease is characterized by an infantile episode of massive subperiosteal new bone formation that typically involves the diaphyses of the long bones, mandible, and clavicles. The involved bones may also appear inflamed, with painful swelling and systemic fever often accompanying the illness. The bone changes usually begin before 5 months of age and resolve before 2 years of age.
    Defects in COL1A1 are a cause of Ehlers-Danlos syndrome type 1 (EDS1) [MIM:130000]; also known as Ehlers-Danlos syndrome gravis. EDS is a connective tissue disorder characterized by hyperextensible skin, atrophic cutaneous scars due to tissue fragility and joint hyperlaxity. EDS1 is the severe form of classic Ehlers-Danlos syndrome.
    Defects in COL1A1 are the cause of Ehlers-Danlos syndrome type 7A (EDS7A) [MIM:130060]; also known as autosomal dominant Ehlers-Danlos syndrome type VII. EDS is a connective tissue disorder characterized by hyperextensible skin, atrophic cutaneous scars due to tissue fragility and joint hyperlaxity. EDS7A is marked by bilateral congenital hip dislocation, hyperlaxity of the joints, and recurrent partial dislocations.
    Defects in COL1A1 are a cause of osteogenesis imperfecta type 1 (OI1) [MIM:166200]. A dominantly inherited connective tissue disorder characterized by bone fragility and blue sclerae. Osteogenesis imperfecta type 1 is non-deforming with normal height or mild short stature, and no dentinogenesis imperfecta.
    Defects in COL1A1 are a cause of osteogenesis imperfecta type 2A (OI2A) [MIM:166210]; also known as osteogenesis imperfecta congenita. A connective tissue disorder characterized by bone fragility, with many perinatal fractures, severe bowing of long bones, undermineralization, and death in the perinatal period due to respiratory insufficiency.
    Defects in COL1A1 are a cause of osteogenesis imperfecta type 3 (OI3) [MIM:259420]. A connective tissue disorder characterized by progressively deforming bones, very short stature, a triangular face, severe scoliosis, grayish sclera, and dentinogenesis imperfecta.
    Defects in COL1A1 are a cause of osteogenesis imperfecta type 4 (OI4) [MIM:166220]; also known as osteogenesis imperfecta with normal sclerae. A connective tissue disorder characterized by moderately short stature, mild to moderate scoliosis, grayish or white sclera and dentinogenesis imperfecta.
    Genetic variations in COL1A1 are a cause of susceptibility to osteoporosis (OSTEOP) [MIM:166710]; also known as involutional or senile osteoporosis or postmenopausal osteoporosis. Osteoporosis is characterized by reduced bone mass, disruption of bone microarchitecture without alteration in the composition of bone. Osteoporotic bones are more at risk of fracture.
    Note=A chromosomal aberration involving COL1A1 is found in dermatofibrosarcoma protuberans. Translocation t(17;22)(q22;q13) with PDGF.
  • Sequence similaritiesBelongs to the fibrillar collagen family.
    Contains 1 fibrillar collagen NC1 domain.
    Contains 1 VWFC domain.
  • Post-translational
    modifications
    Proline residues at the third position of the tripeptide repeating unit (G-X-Y) are hydroxylated in some or all of the chains. Proline residues at the second position of the tripeptide repeating unit (G-X-Y) are hydroxylated in some of the chains.
    O-linked glycan consists of a Glc-Gal disaccharide bound to the oxygen atom of a post-translationally added hydroxyl group.
  • Cellular localizationSecreted > extracellular space > extracellular matrix.
  • Target information above from: UniProt accession P02452 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Database links
  • Alternative names
    • Alpha 1 type I collagen antibody
    • Alpha 2 type I collagen antibody
    • Alpha-1 type I collagen antibody
    • CO1A1_HUMAN antibody
    • COL1A1 antibody
    • COL1A2 antibody
    • Collagen alpha-1(I) chain antibody
    • Collagen I alpha 1 polypeptide antibody
    • Collagen I alpha 2 polypeptide antibody
    • Collagen Of Skin Tendon And Bone antibody
    • Collagen Type 1 antibody
    • Collagen type I alpha 1 antibody
    • Collagen type I alpha 2 antibody
    • OI4 antibody
    • Osteogenesis Imperfecta Type IV antibody
    • Pro alpha 1(I) collagen antibody
    • Type I procollagen antibody
    see all

Anti-Collagen I antibody images

  • ab21286 staining Collagen I in mouse embryonic fibroblasts by Flow Cytometry. Cells were fixed with paraformaldehyde and permeabilized with 0.1% Triton X-100 in PBS. The sample was incubated with the primary antibody (1/300 in 0.01M PBS + 1% BSA + 0.5% Triton X-100) for 8 hours at 4°C. ab150097, an Alexa Fluor® 488-conjugated goat anti-rabbit IgG polyclonal (1/1500) was used as the secondary antibody.
    Gating Strategy: Isotype control (black line in histogram).

    See Abreview

  • ab21286 staining collagen 1 in Mouse small intestine tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with paraformaldehyde and blocked with 10% serum for 20 minutes at 23°C; antigen retrieval was by heat mediation. Samples were incubated with primary antibody (1/250 in TBS (1% Tween) + 10% Goat Serum) for 24 hours at 4°C. An Alexa Fluor®488-conjugated Goat anti-rabbit IgG (ab150077) polyclonal was used as the secondary antibody.

    See Abreview

  • ab21286 at 1/200 staining mouse embryonic lung tissue by IHC-P. The tissue was paraformaldehyde fixed and blocked with serum. A heat mediated antigen retrieval step was performed and the tissue was then stained with the antibody for 15 hours. A biotinylated goat anti-rabbit IgG antibody was used as the secondary.

    See Abreview

  • ab21286 at a 1/200 dilution staining Collagen I in mouse parietal tissue by Immunohistochemistry (PFA fixed, frozen sections), incubated for 16 hours at 4°C. Permeabilized with 0.1% Triton. Blocked using 20% serum for 1 hour at room temperature. Secondary used polyclonal donkey conjugated to Alexa Fluor 555 (green).

    See Abreview

  • ab21286 staining Collagen I (red) in Mouse colon tumor cells by ICC/IF (Immunocytochemistry/immunofluorescence). Cells were fixed with paraformaldehyde, permeabilized boiling in citrate buffer and blocked with 1% BSA for 30 minutes at 25°C. Samples were incubated with primary antibody (1/250 in PBS + 1% BSA) for 12 hours at 4°C. ab96897, DyLight®594-conjugated Goat anti-rabbit IgG polyclonal (1/1000) was used as the secondary antibody. Costained with ab53033, Rabbit anti-E Cadherin (green).

    See Abreview

  • Anti-Collagen I antibody (ab21286) at 1/250 dilution + Mouse tail tendon collagen at 4 µg

    Secondary
    goat anti-rabbit IgG-IRDye 800 conjugated at 1/2500 dilution

    Performed under non-reducing conditions.

    Observed band size : 115,120 kDa (why is the actual band size different from the predicted?)


    Exposure time : 3 minutes

    See Abreview

References for Anti-Collagen I antibody (ab21286)

This product has been referenced in:
  • Breynaert C  et al. Unique gene expression and MR T2 relaxometry patterns define chronic murine dextran sodium sulphate colitis as a model for connective tissue changes in human Crohn's disease. PLoS One 8:e68876 (2013). IHC ; Mouse . Read more (PubMed: 23894361) »
  • Perry SW  et al. Stromal matrix metalloprotease-13 knockout alters Collagen I structure at the tumor-host interface and increases lung metastasis of C57BL/6 syngeneic E0771 mammary tumor cells. BMC Cancer 13:411 (2013). IHC ; Mouse . Read more (PubMed: 24010522) »

See all 10 Publications for this product

Product Wall

Application Flow Cytometry
Fixation Paraformaldehyde
Permeabilization Yes - 0.1% Triton in PBS
Sample Mouse Cell (Mouse Embryonic Fibroblasts (MEFs))
Specification Mouse Embryonic Fibroblasts (MEFs)
Gating Strategy isotype control (black line in histogram)
Preparation Cell harvesting/tissue preparation method: trypsin/edta
Sample buffer: PBS + 10% FBS for washing
Username

Abcam user community

Verified customer

Submitted Mar 04 2014

The concentration of ab21286 is not determined. This product is sold by volume and not by concentration.

This product is sold by volume and not by concentration.

Application Immunocytochemistry/ Immunofluorescence
Blocking step BSA as blocking agent for 30 minute(s) · Concentration: 1% · Temperature: 25°C
Sample Mouse Cell (colon tumor)
Specification colon tumor
Permeabilization Yes - Boiling in citrate buffer
Fixative Paraformaldehyde
Username

Dr. Charles Pallangyo

Verified customer

Submitted Jun 25 2013

I tried to find the mouse and human specific skin cell markers, regrettably I couldn't find enough information about protein that are specific to human or mouse cells. I am sure further publication search will be helpful in answering this question. I w...

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Thank you for contacting us. You are correct, these samples were prepared without the use of B-mercaptoethanol or DTT. Often this is recommended when detecting Collagen.

I hope this information is helpful to you. Please do not hesitate to co...

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Thank you for taking time to complete our questionnaire. I am sorry to hear that you have been having some problems with the Anti-Collagen I antibody (ab21286).

The details provided will enable us to investigate this case and will provide us w...

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Thanks for your email.

Each of these 3 antibodies looks like it would be useful for your experiment. We will guarantee all of them to perform as described on the online datasheet. So the important thing is that you and your colleague are comf...

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Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample Mouse Tissue sections (Colon)
Specification Colon
Fixative Formaldehyde
Antigen retrieval step Heat mediated - Buffer/Enzyme Used: 0.01 M Citrate pH 6
Permeabilization No
Blocking step Serum as blocking agent for 45 minute(s) · Concentration: 5% · Temperature: 20°C
Username

Mr. Jonathan Cremer

Verified customer

Submitted May 05 2012

Application Western blot
Sample Mouse Purified protein (Mouse Tail Tendon Collagen)
Loading amount 4 µg
Specification Mouse Tail Tendon Collagen
Gel Running Conditions Non-reduced Denaturing (4-12% Bis-Tris Gel)
Blocking step Odyssey Blocking Buffer as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: 25°C
Username

Abcam user community

Verified customer

Submitted Nov 18 2011

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"