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Does not react withRat, Chicken, Human
Collagen type I extracted and purified from mouse skin.
Our Abpromise guarantee covers the use of ab21286 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|Flow Cyt||Use at an assay dependent concentration.|
|IHC-Fr||1/40. Tested using a FITC-labeled anti-rabbit IgG secondary.|
|ELISA||Use at an assay dependent concentration.|
|WB||Use at an assay dependent concentration.|
|ICC/IF||Use at an assay dependent concentration.|
|IHC-P||1/250. Tested using an HRP-labeled anti-rabbit IgG secondary.|
ab21286 staining Collagen I in mouse embryonic fibroblasts by Flow Cytometry. Cells were fixed with paraformaldehyde and permeabilized with 0.1% Triton X-100 in PBS. The sample was incubated with the primary antibody (1/300 in 0.01M PBS + 1% BSA + 0.5% Triton X-100) for 8 hours at 4°C. ab150097, an Alexa Fluor® 488-conjugated goat anti-rabbit IgG polyclonal (1/1500) was used as the secondary antibody.
Gating Strategy: Isotype control (black line in histogram).
ab21286 staining collagen 1 in Mouse small intestine tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with paraformaldehyde and blocked with 10% serum for 20 minutes at 23°C; antigen retrieval was by heat mediation. Samples were incubated with primary antibody (1/250 in TBS (1% Tween) + 10% Goat Serum) for 24 hours at 4°C. An Alexa Fluor®488-conjugated Goat anti-rabbit IgG (ab150077) polyclonal was used as the secondary antibody.
ab21286 staining Collagen I (red) in Mouse colon tumor cells by ICC/IF (Immunocytochemistry/immunofluorescence). Cells were fixed with paraformaldehyde, permeabilized boiling in citrate buffer and blocked with 1% BSA for 30 minutes at 25°C. Samples were incubated with primary antibody (1/250 in PBS + 1% BSA) for 12 hours at 4°C. ab96897, DyLight®594-conjugated Goat anti-rabbit IgG polyclonal (1/1000) was used as the secondary antibody. Costained with ab53033, Rabbit anti-E Cadherin (green).
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