Overview

  • Product nameAnti-Collagen I antibody
    See all Collagen I primary antibodies
  • Description
    Rabbit polyclonal to Collagen I
  • SpecificitySpecificity was ascertained by competition ELISA. Complete inhibition was found if the antibody was preincubated with rat collagen type I. Inhibition by rat collagen type III is observed only at 10-20 times higher concentration. No inhibition is found with fibrinectin. fibrogen and laminin.
  • Tested applicationsIHC-Fr, ICC/IF, Dot Blot, ELISAmore details
  • Species reactivity
    Reacts with: Rat
  • Immunogen

    Antibodies to rat collagen type I are raised in rabbits which are numerously immunized with extensively purified native collagen type I extracted from rat tail tendon into dilute acidic buffer after mild pepsin digestion.

Properties

  • FormLiquid
  • Storage instructionsStore at +4°C short term (1-2 weeks). Aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
  • Storage bufferpH: 7.40
  • Concentration information loading...
  • PurityImmunogen affinity purified
  • Purification notesPooled antisera are passed over DEAE-cellulose to produce IgG-enriched fraction. Next, the antisera fraction is absorbed with immunobilized rat collagen types III to remove cross-reactive antibodies to antigenic determinants common for this type of collagen. The affinity purified antibody ab24133 is obtained by binding to immobilized native rat collagen type I (the antigen used for immunization), followed by elution with acidic buffer, neutralisation, dialysis, dispensing and lyophilization.
  • Clonality Polyclonal
  • IsotypeIgG
  • Research Areas

Applications

Our Abpromise guarantee covers the use of ab24133 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-Fr 1/20.
ICC/IF Use at an assay dependent dilution.
Dot Blot Use at an assay dependent dilution.
ELISA Use at an assay dependent dilution.

Target

  • FunctionType I collagen is a member of group I collagen (fibrillar forming collagen).
  • Tissue specificityForms the fibrils of tendon, ligaments and bones. In bones the fibrils are mineralized with calcium hydroxyapatite.
  • Involvement in diseaseDefects in COL1A1 are the cause of Caffey disease (CAFFD) [MIM:114000]; also known as infantile cortical hyperostosis. Caffey disease is characterized by an infantile episode of massive subperiosteal new bone formation that typically involves the diaphyses of the long bones, mandible, and clavicles. The involved bones may also appear inflamed, with painful swelling and systemic fever often accompanying the illness. The bone changes usually begin before 5 months of age and resolve before 2 years of age.
    Defects in COL1A1 are a cause of Ehlers-Danlos syndrome type 1 (EDS1) [MIM:130000]; also known as Ehlers-Danlos syndrome gravis. EDS is a connective tissue disorder characterized by hyperextensible skin, atrophic cutaneous scars due to tissue fragility and joint hyperlaxity. EDS1 is the severe form of classic Ehlers-Danlos syndrome.
    Defects in COL1A1 are the cause of Ehlers-Danlos syndrome type 7A (EDS7A) [MIM:130060]; also known as autosomal dominant Ehlers-Danlos syndrome type VII. EDS is a connective tissue disorder characterized by hyperextensible skin, atrophic cutaneous scars due to tissue fragility and joint hyperlaxity. EDS7A is marked by bilateral congenital hip dislocation, hyperlaxity of the joints, and recurrent partial dislocations.
    Defects in COL1A1 are a cause of osteogenesis imperfecta type 1 (OI1) [MIM:166200]. A dominantly inherited connective tissue disorder characterized by bone fragility and blue sclerae. Osteogenesis imperfecta type 1 is non-deforming with normal height or mild short stature, and no dentinogenesis imperfecta.
    Defects in COL1A1 are a cause of osteogenesis imperfecta type 2A (OI2A) [MIM:166210]; also known as osteogenesis imperfecta congenita. A connective tissue disorder characterized by bone fragility, with many perinatal fractures, severe bowing of long bones, undermineralization, and death in the perinatal period due to respiratory insufficiency.
    Defects in COL1A1 are a cause of osteogenesis imperfecta type 3 (OI3) [MIM:259420]. A connective tissue disorder characterized by progressively deforming bones, very short stature, a triangular face, severe scoliosis, grayish sclera, and dentinogenesis imperfecta.
    Defects in COL1A1 are a cause of osteogenesis imperfecta type 4 (OI4) [MIM:166220]; also known as osteogenesis imperfecta with normal sclerae. A connective tissue disorder characterized by moderately short stature, mild to moderate scoliosis, grayish or white sclera and dentinogenesis imperfecta.
    Genetic variations in COL1A1 are a cause of susceptibility to osteoporosis (OSTEOP) [MIM:166710]; also known as involutional or senile osteoporosis or postmenopausal osteoporosis. Osteoporosis is characterized by reduced bone mass, disruption of bone microarchitecture without alteration in the composition of bone. Osteoporotic bones are more at risk of fracture.
    Note=A chromosomal aberration involving COL1A1 is found in dermatofibrosarcoma protuberans. Translocation t(17;22)(q22;q13) with PDGF.
  • Sequence similaritiesBelongs to the fibrillar collagen family.
    Contains 1 fibrillar collagen NC1 domain.
    Contains 1 VWFC domain.
  • Post-translational
    modifications
    Proline residues at the third position of the tripeptide repeating unit (G-X-Y) are hydroxylated in some or all of the chains. Proline residues at the second position of the tripeptide repeating unit (G-X-Y) are hydroxylated in some of the chains.
    O-linked glycan consists of a Glc-Gal disaccharide bound to the oxygen atom of a post-translationally added hydroxyl group.
  • Cellular localizationSecreted > extracellular space > extracellular matrix.
  • Information by UniProt
  • Database links
  • Alternative names
    • Alpha 1 type I collagen antibody
    • Alpha 2 type I collagen antibody
    • Alpha-1 type I collagen antibody
    • CO1A1_HUMAN antibody
    • COL1A1 antibody
    • COL1A2 antibody
    • Collagen alpha-1(I) chain antibody
    • Collagen I alpha 1 polypeptide antibody
    • Collagen I alpha 2 polypeptide antibody
    • Collagen Of Skin Tendon And Bone antibody
    • Collagen Type 1 antibody
    • Collagen type I alpha 1 antibody
    • Collagen type I alpha 2 antibody
    • OI4 antibody
    • Osteogenesis Imperfecta Type IV antibody
    • Pro alpha 1(I) collagen antibody
    • Type I procollagen antibody
    see all

References for Anti-Collagen I antibody (ab24133)

This product has been referenced in:
  • Serpooshan V  et al. Hydraulic permeability of multilayered collagen gel scaffolds under plastic compression-induced unidirectional fluid flow. Acta Biomater 9:4673-80 (2013). ICC/IF . Read more (PubMed: 22947324) »
  • Tai BC  et al. Modified polyelectrolyte complex fibrous scaffold as a matrix for 3D cell culture. Biomaterials 31:5927-35 (2010). ICC/IF . Read more (PubMed: 20472284) »

See all 3 Publications for this product

Product Wall

Thank you for your calls today and for your questions about this antibody.

The assay sounds similar to an indirect ELISA, with the protocol below:

http://www.abcam.com/index.html?pageconfig=resource&rid=11389

I have em...

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Thank you for contacting us in regards to Anti-Collagen I antibodies to be used in rat samples. As discussed over the phone ab24133 has been used for immunofluorescence and IHC with frozen sections. It has also been tested for specificity with rat ...

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