Anti-Collagen I antibody (ab292)
- Product nameAnti-Collagen I antibodySee all Collagen I primary antibodies ...
- DescriptionRabbit polyclonal to Collagen I
- SpecificityThere may be cross reactivity with other forms of collagen. Some class specific anti-collagens may be specific for three-dimensional epitopes which may result in diminished reactivity with denatured collagen or formalin-fixed, paraffin embedded tissues. This antibody reacts with most mammalian Type I collagens and has negligible cross-reactivity with Type II, III, IV, V or VI collagens. Non-specific cross-reaction of anti-collagen antibodies with other human serum proteins or non-collagen extracellular matrix proteins is negligible.
- Tested applicationsWB, ELISA, IHC-Fr, ICC/IF, IHC-P, ICC more details
- Species reactivityReacts with: Mouse, Rat, Horse, Cow, Human
Full length protein - Collagen Type I from human and bovine placenta
- Positive controlWistar Rat hepatic stellate cell lysate [GFP transduced], for Western blotting.
- General notesThe SDS-PAGE/ Coomassie stained gel of collagen I has two bands at 138K and 129K non-reduced.
It is often extremely difficult to generate antibodies with specificities to collagens due to the uninterrupted "Glycine-X-Y" triplet repeat that is a necessary part of the triple helical structure. The development of type specific antibodies is dependent on NON-DENATURED three-dimensional epitopes - this may result in diminished reactivity of some antibodies with denatured collagen or formalin-fixed, paraffin embedded tissues. Anti-Collagen antibodies have been used for indirect trapping ELISA for quantitation of antigen in serum using a standard curve, for immunoprecipitation and for native (non-denaturing, non-dissociating) PAGE and western blotting for highly sensitive qualitative analysis.
- Storage instructionsStore at +4°C short term (1-2 weeks). Aliquot and store at -20°C or -80°C (add glycerol to a final volume of 50% for extra stability). Avoid repeated freeze / thaw cycles.
- Storage bufferPreservative: 0.01% Sodium Azide
Constituents: 0.125M Sodium Borate, 0.075M Sodium Chloride, 0.005M EDTA. pH 8.0
- Concentration information loading...
- PurityImmunogen affinity purified
- Purification notesThis product was prepared by immunoaffinity chromatography on immobilized antigens followed by extensive cross-adsorption against other collagens, human serum proteins and non-collagen extracellular matrix proteins to remove unwanted specificities. This product has been sterile filtered.
- Primary antibody notes It is often extremely difficult to generate antibodies with specificities to collagens due to the uninterrupted "Glycine-X-Y" triplet repeat that is a necessary part of the triple helical structure. The development of type specific antibodies is dependent on NON-DENATURED three-dimensional epitopes - this may result in diminished reactivity of some antibodies with denatured collagen or formalin-fixed, paraffin embedded tissues. Anti-Collagen antibodies have been used for indirect trapping ELISA for quantitation of antigen in serum using a standard curve, for immunoprecipitation and for native (non-denaturing, non-dissociating) PAGE and western blotting for highly sensitive qualitative analysis.
- Clonality Polyclonal
- Research Areas
Our Abpromise guarantee covers the use of ab292 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||WB: 1/5000 - 1/50000. Detects a band of approximately 80-120 kDa. This product is not recommended for use under denaturing conditions in WB and ELISA. We would suggest testing it under native conditions.|
|ELISA||ELISA: 1/5000 - 1/50000.|
|IHC-Fr||IHC-Fr: 1/50 - 1/200.|
|IHC-P||IHC-P: 1/200. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.|
|ICC||ICC: Use at an assay dependent dilution.|
- FunctionType I collagen is a member of group I collagen (fibrillar forming collagen).
- Tissue specificityForms the fibrils of tendon, ligaments and bones. In bones the fibrils are mineralized with calcium hydroxyapatite.
- Involvement in diseaseDefects in COL1A1 are the cause of Caffey disease (CAFFD) [MIM:114000]; also known as infantile cortical hyperostosis. Caffey disease is characterized by an infantile episode of massive subperiosteal new bone formation that typically involves the diaphyses of the long bones, mandible, and clavicles. The involved bones may also appear inflamed, with painful swelling and systemic fever often accompanying the illness. The bone changes usually begin before 5 months of age and resolve before 2 years of age.
Defects in COL1A1 are a cause of Ehlers-Danlos syndrome type 1 (EDS1) [MIM:130000]; also known as Ehlers-Danlos syndrome gravis. EDS is a connective tissue disorder characterized by hyperextensible skin, atrophic cutaneous scars due to tissue fragility and joint hyperlaxity. EDS1 is the severe form of classic Ehlers-Danlos syndrome.
Defects in COL1A1 are the cause of Ehlers-Danlos syndrome type 7A (EDS7A) [MIM:130060]; also known as autosomal dominant Ehlers-Danlos syndrome type VII. EDS is a connective tissue disorder characterized by hyperextensible skin, atrophic cutaneous scars due to tissue fragility and joint hyperlaxity. EDS7A is marked by bilateral congenital hip dislocation, hyperlaxity of the joints, and recurrent partial dislocations.
Defects in COL1A1 are a cause of osteogenesis imperfecta type 1 (OI1) [MIM:166200]. A dominantly inherited connective tissue disorder characterized by bone fragility and blue sclerae. Osteogenesis imperfecta type 1 is non-deforming with normal height or mild short stature, and no dentinogenesis imperfecta.
Defects in COL1A1 are a cause of osteogenesis imperfecta type 2A (OI2A) [MIM:166210]; also known as osteogenesis imperfecta congenita. A connective tissue disorder characterized by bone fragility, with many perinatal fractures, severe bowing of long bones, undermineralization, and death in the perinatal period due to respiratory insufficiency.
Defects in COL1A1 are a cause of osteogenesis imperfecta type 3 (OI3) [MIM:259420]. A connective tissue disorder characterized by progressively deforming bones, very short stature, a triangular face, severe scoliosis, grayish sclera, and dentinogenesis imperfecta.
Defects in COL1A1 are a cause of osteogenesis imperfecta type 4 (OI4) [MIM:166220]; also known as osteogenesis imperfecta with normal sclerae. A connective tissue disorder characterized by moderately short stature, mild to moderate scoliosis, grayish or white sclera and dentinogenesis imperfecta.
Genetic variations in COL1A1 are a cause of susceptibility to osteoporosis (OSTEOP) [MIM:166710]; also known as involutional or senile osteoporosis or postmenopausal osteoporosis. Osteoporosis is characterized by reduced bone mass, disruption of bone microarchitecture without alteration in the composition of bone. Osteoporotic bones are more at risk of fracture.
Note=A chromosomal aberration involving COL1A1 is found in dermatofibrosarcoma protuberans. Translocation t(17;22)(q22;q13) with PDGF.
- Sequence similaritiesBelongs to the fibrillar collagen family.
Contains 1 fibrillar collagen NC1 domain.
Contains 1 VWFC domain.
modificationsProline residues at the third position of the tripeptide repeating unit (G-X-Y) are hydroxylated in some or all of the chains. Proline residues at the second position of the tripeptide repeating unit (G-X-Y) are hydroxylated in some of the chains.
O-linked glycan consists of a Glc-Gal disaccharide bound to the oxygen atom of a post-translationally added hydroxyl group.
- Cellular localizationSecreted > extracellular space > extracellular matrix.
- Entrez Gene: 282187 Cow
- Entrez Gene: 282188 Cow
- Entrez Gene: 1278 Human
- Entrez Gene: 1277 Human
- Entrez Gene: 12843 Mouse
- Entrez Gene: 12842 Mouse
- Entrez Gene: 84352 Rat
- Entrez Gene: 29393 Rat
- Omim: 120150 Human
- Omim: 120160 Human
- SwissProt: P02465 Cow
- SwissProt: P02453 Cow
- SwissProt: P08123 Human
- SwissProt: P02452 Human
- SwissProt: Q01149 Mouse
- SwissProt: P11087 Mouse
- SwissProt: P02466 Rat
- SwissProt: P02454 Rat
- Unigene: 489142 Human
- Unigene: 172928 Human
- Unigene: 681002 Human
- Unigene: 277735 Mouse
- Unigene: 458212 Mouse
- Unigene: 107239 Rat
- Unigene: 2953 Rat
- Alpha 1 type I collagen antibodyAlpha 2 type I collagen antibodyAlpha-1 type I collagen antibody
- CO1A1_HUMAN antibodyCOL1A1 antibodyCOL1A2 antibodyCollagen alpha-1(I) chain antibodyCollagen I alpha 1 polypeptide antibodyCollagen I alpha 2 polypeptide antibodyCollagen Of Skin Tendon And Bone antibodyCollagen Type 1 antibodyCollagen type I alpha 1 antibodyCollagen type I alpha 2 antibodyOI4 antibodyOsteogenesis Imperfecta Type IV antibodyPro alpha 1(I) collagen antibodyType I procollagen antibody
Anti-Collagen I antibody images
ab292 at 1/200 staining rat testes tissue sections by Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections). The tissue was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed, before a blocking step in 1% serum. The tissue was incuabted with the primary antibody for 30 minutes at 22°C and then with the HRP conjugated goat anti-rabbit secondary.
ab292 staining Collagen I in horse bronchial fibroblast cells by Immunocytochemistry. Cells were fixed with acetone and blocking with 3% BSA was performed for 1 hour 30 minutes at 40C. Samples were incubated with primary antibody (1/500: in 3% BSA/ PBS) for 12 hours at 4°C. An FITC-conjugated goat polyclonal to rabbit IgG was used at dilution at 1/160 as secondary antibody.
ab292 staining Collagen I in murine endochondral bone (Rib) by Immunohistochemistry (Frozen sections). Tissue was fixed with paraformaldehyde and permeabilized using 0.1% Triton. Samples were then blocked with 10% serum for 1 hour at 19°C followed by incubation with the primary antibody at a 1/200 dilution for 16 hours at 4°C. An Alexa Fluor® conjugated donkey anti-rabbit polyclonal was used as secondary antibody at a 1/200 dilution. Counterstain DAPI (blue).
References for Anti-Collagen I antibody (ab292)
This product has been referenced in:
- Ulsamer A et al. Axin Pathway Activity Regulates in Vivo pY654-ß-catenin Accumulation and Pulmonary Fibrosis. J Biol Chem 287:5164-72 (2012). Read more (PubMed: 22203675) »
- Zeng M et al. Lymphoid tissue damage in HIV-1 infection depletes naïve T cells and limits T cell reconstitution after antiretroviral therapy. PLoS Pathog 8:e1002437 (2012). Read more (PubMed: 22241988) »