Anti-Collagen I antibody (ab59435)

Overview

• Product nameAnti-Collagen I antibodySee all Collagen I primary antibodies ...
• Description
  Rabbit polyclonal to Collagen I
• SpecificityDetects endogenous levels of total Collagen I protein.
• Tested applicationsELISA, ICC/IF, IHC-Fr, WB more details
• Species reactivity
  Reacts with: Mouse, Rat, Human
  
• Immunogen

  Synthetic peptide (Human) from the N-terminal of Collagen I.

• Positive controlNIH/3T3 cells

Properties

• FormLiquid
• Storage instructionsStore at -20°C. Stable for 12 months at -20°C
• Storage bufferPreservative: 0.02% Sodium Azide
  Constituents: 50% Glycerol, PBS (without Mg²⁺ and Ca²⁺), 150mM Sodium chloride, pH 7.4
• Concentration information loading...
• PurityImmunogen affinity purified
• Purification notesAffinity purified from rabbit antiserum by affinity chromatography using epitope specific immunogen.
• Clonality Polyclonal
• IsotypeIgG
• Research Areas
  • Stem Cells
  • Mesenchymal Stem Cells
  • Osteogenesis

  • Signal Transduction
  • Cytoskeleton / ECM
  • Extracellular Matrix
  • ECM Proteins
  • Collagen

Applications

Target

• FunctionType I collagen is a member of group I collagen (fibrillar forming collagen).
• Tissue specificityForms the fibrils of tendon, ligaments and bones. In bones the fibrils are mineralized with calcium hydroxyapatite.
• Involvement in diseaseDefects in COL1A1 are the cause of Caffey disease (CAFFD) [MIM:114000]; also known as infantile cortical hyperostosis. Caffey disease is characterized by an infantile episode of massive subperiosteal new bone formation that typically involves the diaphyses of the long bones, mandible, and clavicles. The involved bones may also appear inflamed, with painful swelling and systemic fever often accompanying the illness. The bone changes usually begin before 5 months of age and resolve before 2 years of age.
  Defects in COL1A1 are a cause of Ehlers-Danlos syndrome type 1 (EDS1) [MIM:130000]; also known as Ehlers-Danlos syndrome gravis. EDS is a connective tissue disorder characterized by hyperextensible skin, atrophic cutaneous scars due to tissue fragility and joint hyperlaxity. EDS1 is the severe form of classic Ehlers-Danlos syndrome.
  Defects in COL1A1 are the cause of Ehlers-Danlos syndrome type 7A (EDS7A) [MIM:130060]; also known as autosomal dominant Ehlers-Danlos syndrome type VII. EDS is a connective tissue disorder characterized by hyperextensible skin, atrophic cutaneous scars due to tissue fragility and joint hyperlaxity. EDS7A is marked by bilateral congenital hip dislocation, hyperlaxity of the joints, and recurrent partial dislocations.
  Defects in COL1A1 are a cause of osteogenesis imperfecta type 1 (OI1) [MIM:166200]. A dominantly inherited connective tissue disorder characterized by bone fragility and blue sclerae. Osteogenesis imperfecta type 1 is non-deforming with normal height or mild short stature, and no dentinogenesis imperfecta.
  Defects in COL1A1 are a cause of osteogenesis imperfecta type 2A (OI2A) [MIM:166210]; also known as osteogenesis imperfecta congenita. A connective tissue disorder characterized by bone fragility, with many perinatal fractures, severe bowing of long bones, undermineralization, and death in the perinatal period due to respiratory insufficiency.
  Defects in COL1A1 are a cause of osteogenesis imperfecta type 3 (OI3) [MIM:259420]. A connective tissue disorder characterized by progressively deforming bones, very short stature, a triangular face, severe scoliosis, grayish sclera, and dentinogenesis imperfecta.
  Defects in COL1A1 are a cause of osteogenesis imperfecta type 4 (OI4) [MIM:166220]; also known as osteogenesis imperfecta with normal sclerae. A connective tissue disorder characterized by moderately short stature, mild to moderate scoliosis, grayish or white sclera and dentinogenesis imperfecta.
  Genetic variations in COL1A1 are a cause of susceptibility to osteoporosis (OSTEOP) [MIM:166710]; also known as involutional or senile osteoporosis or postmenopausal osteoporosis. Osteoporosis is characterized by reduced bone mass, disruption of bone microarchitecture without alteration in the composition of bone. Osteoporotic bones are more at risk of fracture.
  Note=A chromosomal aberration involving COL1A1 is found in dermatofibrosarcoma protuberans. Translocation t(17;22)(q22;q13) with PDGF.
• Sequence similaritiesBelongs to the fibrillar collagen family.
  Contains 1 fibrillar collagen NC1 domain.
  Contains 1 VWFC domain.
• Post-translational
  modificationsProline residues at the third position of the tripeptide repeating unit (G-X-Y) are hydroxylated in some or all of the chains. Proline residues at the second position of the tripeptide repeating unit (G-X-Y) are hydroxylated in some of the chains.
  O-linked glycan consists of a Glc-Gal disaccharide bound to the oxygen atom of a post-translationally added hydroxyl group.
• Cellular localizationSecreted > extracellular space > extracellular matrix.

Target information above from: UniProt accession P02452 The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010) .

Information by UniProt
• Database links
  • Entrez Gene: 1278 Human
  • Entrez Gene: 1277 Human
  • Entrez Gene: 12843 Mouse
  • Entrez Gene: 12842 Mouse
  • Entrez Gene: 84352 Rat
  • Entrez Gene: 29393 Rat
  • Omim: 120150 Human
  • Omim: 120160 Human

  • SwissProt: P08123 Human
  • SwissProt: P02452 Human
  • SwissProt: Q01149 Mouse
  • SwissProt: P11087 Mouse
  • SwissProt: P02466 Rat
  • SwissProt: P02454 Rat
  • Unigene: 489142 Human
  • Unigene: 172928 Human
  • Unigene: 681002 Human
  • Unigene: 277735 Mouse
  • Unigene: 458212 Mouse
  • Unigene: 107239 Rat
  • Unigene: 2953 Rat

  see all

• Alternative names
  Alpha 1 type I collagen antibodyAlpha 2 type I collagen antibodyAlpha-1 type I collagen antibody

  CO1A1_HUMAN antibodyCOL1A1 antibodyCOL1A2 antibodyCollagen alpha-1(I) chain antibodyCollagen I alpha 1 polypeptide antibodyCollagen I alpha 2 polypeptide antibodyCollagen Of Skin Tendon And Bone antibodyCollagen Type 1 antibodyCollagen type I alpha 1 antibodyCollagen type I alpha 2 antibodyOI4 antibodyOsteogenesis Imperfecta Type IV antibodyPro alpha 1(I) collagen antibodyType I procollagen antibody

  see all

Anti-Collagen I antibody images

• 

  Immunofluorescence - Collagen I antibody (ab59435)
  Immunofluorescence analysis of NIH/3T3 cells using ab59435 at 1/500 dilution. Samples were treated -/+ phosphopeptide.
• 

  Immunohistochemistry (Frozen sections) - Collagen I antibody (ab59435)This image is courtesy of an anonymous Abreview
  ab59435 at 1/200 dilution staining Collagen I in mouse dermis tissue by immunohistochemistry (frozen sections). Sections were paraformaldeyde fixed, permeabilized in 0.1% Triton prior to blocking in 20% serum for 1 hour at RT and then incubated with ab59435 for 16 hours at 4°C. Alexa fluor® 555 donkey polyclonal, diluted 1/200, was used as the secondary antibody.

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• 

  Western blot - Anti-Collagen I antibody (ab59435)
  All lanes : Anti-Collagen I antibody (ab59435) at 1/200 dilution
  
  Lane 1 : Whole tissue lysate prepared from untreated rat liver fibrosis.
  Lane 2 : Whole tissue lysate prepared from rat liver fibrosis treated with a low dose of TFO.
  Lane 3 : Whole tissue lysate prepared from rat liver fibrosis treated with a high dose of TFO.
  
  Lysates/proteins at 20 µg per lane.
  
  Secondary
  HRP conjugated goat anti-rabbit polyclonal at 1/10000 dilution
  developed using the ECL technique
  
  Predicted band size : 130 kDa
  
  
  Exposure time : 1 minute

  Image courtesy of an anonymous Abreview.

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