Overview
- Product nameAnti-Collagen I antibodySee all Collagen I primary antibodies ...
- DescriptionRabbit polyclonal to Collagen I
- Tested applicationsIP, WB, IHC-P, ICC/IF more details
- Species reactivityReacts with: Mouse, Rat
Predicted to work with: Human - Immunogen
Synthetic peptide conjugated to KLH derived from within residues 1 - 100 of Human Collagen I.
(Peptide available as ab101220.)
- Positive controlThis antibody gave a positive signal in Mouse Skin and Rat Kidney This antibody gave a positive result in IF in the following Formaldehyde fixed cell line: MEF1
Properties
- FormLiquid
- Storage instructionsStore at +4°C short term (1-2 weeks). Aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
- Storage bufferpH: 7.40
Preservative: 0.02% Sodium azide
Constituent: PBS
Note: Batches of this product that have a concentration < 1mg/ml may have BSA added as a stabilising agent. If you would like information about the formulation of a specific lot, please contact our scientific support team who will be happy to help. -
Concentration information loading... - PurityImmunogen affinity purified
- Clonality Polyclonal
- IsotypeIgG
- Research Areas
Applications
Our Abpromise guarantee covers the use of ab84956 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Notes |
|---|---|
| IP | IP: Use a concentration of 5 µg/ml. |
| WB | WB: Use a concentration of 1 µg/ml. Detects a band of approximately 122 kDa (predicted molecular weight: 138 kDa).Can be blocked with Collagen I peptide (ab101220). |
| IHC-P | IHC-P: Use a concentration of 5 µg/ml. |
| ICC/IF | ICC/IF: Use a concentration of 5 µg/ml. |
Target
- FunctionType I collagen is a member of group I collagen (fibrillar forming collagen).
- Tissue specificityForms the fibrils of tendon, ligaments and bones. In bones the fibrils are mineralized with calcium hydroxyapatite.
- Involvement in diseaseDefects in COL1A1 are the cause of Caffey disease (CAFFD) [MIM:114000]; also known as infantile cortical hyperostosis. Caffey disease is characterized by an infantile episode of massive subperiosteal new bone formation that typically involves the diaphyses of the long bones, mandible, and clavicles. The involved bones may also appear inflamed, with painful swelling and systemic fever often accompanying the illness. The bone changes usually begin before 5 months of age and resolve before 2 years of age.
Defects in COL1A1 are a cause of Ehlers-Danlos syndrome type 1 (EDS1) [MIM:130000]; also known as Ehlers-Danlos syndrome gravis. EDS is a connective tissue disorder characterized by hyperextensible skin, atrophic cutaneous scars due to tissue fragility and joint hyperlaxity. EDS1 is the severe form of classic Ehlers-Danlos syndrome.
Defects in COL1A1 are the cause of Ehlers-Danlos syndrome type 7A (EDS7A) [MIM:130060]; also known as autosomal dominant Ehlers-Danlos syndrome type VII. EDS is a connective tissue disorder characterized by hyperextensible skin, atrophic cutaneous scars due to tissue fragility and joint hyperlaxity. EDS7A is marked by bilateral congenital hip dislocation, hyperlaxity of the joints, and recurrent partial dislocations.
Defects in COL1A1 are a cause of osteogenesis imperfecta type 1 (OI1) [MIM:166200]. A dominantly inherited connective tissue disorder characterized by bone fragility and blue sclerae. Osteogenesis imperfecta type 1 is non-deforming with normal height or mild short stature, and no dentinogenesis imperfecta.
Defects in COL1A1 are a cause of osteogenesis imperfecta type 2A (OI2A) [MIM:166210]; also known as osteogenesis imperfecta congenita. A connective tissue disorder characterized by bone fragility, with many perinatal fractures, severe bowing of long bones, undermineralization, and death in the perinatal period due to respiratory insufficiency.
Defects in COL1A1 are a cause of osteogenesis imperfecta type 3 (OI3) [MIM:259420]. A connective tissue disorder characterized by progressively deforming bones, very short stature, a triangular face, severe scoliosis, grayish sclera, and dentinogenesis imperfecta.
Defects in COL1A1 are a cause of osteogenesis imperfecta type 4 (OI4) [MIM:166220]; also known as osteogenesis imperfecta with normal sclerae. A connective tissue disorder characterized by moderately short stature, mild to moderate scoliosis, grayish or white sclera and dentinogenesis imperfecta.
Genetic variations in COL1A1 are a cause of susceptibility to osteoporosis (OSTEOP) [MIM:166710]; also known as involutional or senile osteoporosis or postmenopausal osteoporosis. Osteoporosis is characterized by reduced bone mass, disruption of bone microarchitecture without alteration in the composition of bone. Osteoporotic bones are more at risk of fracture.
Note=A chromosomal aberration involving COL1A1 is found in dermatofibrosarcoma protuberans. Translocation t(17;22)(q22;q13) with PDGF. - Sequence similaritiesBelongs to the fibrillar collagen family.
Contains 1 fibrillar collagen NC1 domain.
Contains 1 VWFC domain. - Post-translational
modificationsProline residues at the third position of the tripeptide repeating unit (G-X-Y) are hydroxylated in some or all of the chains. Proline residues at the second position of the tripeptide repeating unit (G-X-Y) are hydroxylated in some of the chains.
O-linked glycan consists of a Glc-Gal disaccharide bound to the oxygen atom of a post-translationally added hydroxyl group. - Cellular localizationSecreted > extracellular space > extracellular matrix.
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Database links
- Entrez Gene: 1278 Human
- Entrez Gene: 1277 Human
- Entrez Gene: 12843 Mouse
- Entrez Gene: 12842 Mouse
- Entrez Gene: 84352 Rat
- Entrez Gene: 29393 Rat
- Omim: 120150 Human
- Omim: 120160 Human
- SwissProt: P08123 Human
- SwissProt: P02452 Human
- SwissProt: Q01149 Mouse
- SwissProt: P11087 Mouse
- SwissProt: P02466 Rat
- SwissProt: P02454 Rat
- Unigene: 489142 Human
- Unigene: 172928 Human
- Unigene: 681002 Human
- Unigene: 277735 Mouse
- Unigene: 458212 Mouse
- Unigene: 107239 Rat
- Unigene: 2953 Rat
see all
Target information above from: UniProt accession
P02452
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010)
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Alternative names
- Alpha 1 type I collagen antibodyAlpha 2 type I collagen antibodyAlpha-1 type I collagen antibody
- CO1A1_HUMAN antibodyCOL1A1 antibodyCOL1A2 antibodyCollagen alpha-1(I) chain antibodyCollagen I alpha 1 polypeptide antibodyCollagen I alpha 2 polypeptide antibodyCollagen type I alpha 1 antibodyCollagen type I alpha 2 antibodyType I procollagen antibody
see all
Anti-Collagen I antibody images
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Western blot - Anti-Collagen I antibody (ab84956)All lanes : Anti-Collagen I antibody (ab84956) at 1 µg/ml
Lane 1 :Skin (Mouse) Whole Cell Lysate - normal tissue (ab4025)
Lane 2 : Kidney (Rat) Tissue Lysate
Lysates/proteins at 10 µg per lane.
Secondary
Goat polyclonal Secondary Antibody to Rabbit IgG - H&L (HRP), pre-adsorbed (ab97080) at 1/5000 dilution
developed using the ECL technique
Performed under reducing conditions.
Predicted band size : 138 kDa
Observed band size : 110 kDa (why is the actual band size different from the predicted?)
Additional bands at : 34 kDa. We are unsure as to the identity of these extra bands.
Exposure time : 30 minutes -
Immunoprecipitation - Anti-Collagen I antibody (ab84956)Collagen I was immunoprecipitated using 0.5mg Rat Kidney tissue lysate, 5µg of Rabbit polyclonal to Collagen I and 50µl of protein G magnetic beads (+). No antibody was added to the control (-).
The antibody was incubated under agitation with Protein G beads for 10min, Rat Kidney tissue lysate lysate diluted in RIPA buffer was added to each sample and incubated for a further 10min under agitation.
Proteins were eluted by addition of 40µl SDS loading buffer and incubated for 10min at 70oC; 10µl of each sample was separated on a SDS PAGE gel, transferred to a nitrocellulose membrane, blocked with 5% BSA and probed with ab84956.
Secondary: Mouse monoclonal [SB62a] Secondary Antibody to Rabbit IgG light chain (HRP) (ab99697).
Band: 110kDa; Collagen I -
Immunocytochemistry/ Immunofluorescence - Anti-Collagen I antibody (ab84956)ab84956 stained MEF1 cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody ab84956 at 5µg/ml overnight at +4°C. The secondary antibody (green) was DyLight® 488 goat anti- rabbit (ab96899) IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
References for Anti-Collagen I antibody (ab84956)
ab84956 has not yet been referenced specifically in any publications.
