Anti-Collagen I antibody (ab88147)

Overview

• Product nameAnti-Collagen I antibodySee all Collagen I primary antibodies ...
• Description
  Mouse monoclonal to Collagen I
• Tested applicationsWB, ELISA, IHC-P more details
• Species reactivity
  Reacts with: Mouse, Human
  
• Immunogen

  Recombinant fragment: EGSPGRDGSP GAKGDRGETG PAGPPGAPGA PGAPGPVGPA GKSGDRGETG PAGPAGPVGP VGARGPAGPQ GPRGDKGETG EQGDRGIK, corresponding to amino acids 1021 - 1109 of Human Collagen I (NP_000079), with a proprietary tag

  Run BLAST with Run BLAST with
• Positive controlNIH 3T3 cell lysate IHC-P: human normal skin FFPE tissue sections

Properties

• FormLiquid
• Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
• Storage bufferPreservative: None
  Constituents: PBS, pH 7.2
• Concentration information loading...
• PurityProtein A purified
• Clonality Monoclonal
• IsotypeIgG3
• Light chain typekappa
• Research Areas
  • Stem Cells
  • Mesenchymal Stem Cells
  • Osteogenesis

  • Signal Transduction
  • Cytoskeleton / ECM
  • Extracellular Matrix
  • ECM Proteins
  • Collagen

Applications

Target

• FunctionType I collagen is a member of group I collagen (fibrillar forming collagen).
• Tissue specificityForms the fibrils of tendon, ligaments and bones. In bones the fibrils are mineralized with calcium hydroxyapatite.
• Involvement in diseaseDefects in COL1A1 are the cause of Caffey disease (CAFFD) [MIM:114000]; also known as infantile cortical hyperostosis. Caffey disease is characterized by an infantile episode of massive subperiosteal new bone formation that typically involves the diaphyses of the long bones, mandible, and clavicles. The involved bones may also appear inflamed, with painful swelling and systemic fever often accompanying the illness. The bone changes usually begin before 5 months of age and resolve before 2 years of age.
  Defects in COL1A1 are a cause of Ehlers-Danlos syndrome type 1 (EDS1) [MIM:130000]; also known as Ehlers-Danlos syndrome gravis. EDS is a connective tissue disorder characterized by hyperextensible skin, atrophic cutaneous scars due to tissue fragility and joint hyperlaxity. EDS1 is the severe form of classic Ehlers-Danlos syndrome.
  Defects in COL1A1 are the cause of Ehlers-Danlos syndrome type 7A (EDS7A) [MIM:130060]; also known as autosomal dominant Ehlers-Danlos syndrome type VII. EDS is a connective tissue disorder characterized by hyperextensible skin, atrophic cutaneous scars due to tissue fragility and joint hyperlaxity. EDS7A is marked by bilateral congenital hip dislocation, hyperlaxity of the joints, and recurrent partial dislocations.
  Defects in COL1A1 are a cause of osteogenesis imperfecta type 1 (OI1) [MIM:166200]. A dominantly inherited connective tissue disorder characterized by bone fragility and blue sclerae. Osteogenesis imperfecta type 1 is non-deforming with normal height or mild short stature, and no dentinogenesis imperfecta.
  Defects in COL1A1 are a cause of osteogenesis imperfecta type 2A (OI2A) [MIM:166210]; also known as osteogenesis imperfecta congenita. A connective tissue disorder characterized by bone fragility, with many perinatal fractures, severe bowing of long bones, undermineralization, and death in the perinatal period due to respiratory insufficiency.
  Defects in COL1A1 are a cause of osteogenesis imperfecta type 3 (OI3) [MIM:259420]. A connective tissue disorder characterized by progressively deforming bones, very short stature, a triangular face, severe scoliosis, grayish sclera, and dentinogenesis imperfecta.
  Defects in COL1A1 are a cause of osteogenesis imperfecta type 4 (OI4) [MIM:166220]; also known as osteogenesis imperfecta with normal sclerae. A connective tissue disorder characterized by moderately short stature, mild to moderate scoliosis, grayish or white sclera and dentinogenesis imperfecta.
  Genetic variations in COL1A1 are a cause of susceptibility to osteoporosis (OSTEOP) [MIM:166710]; also known as involutional or senile osteoporosis or postmenopausal osteoporosis. Osteoporosis is characterized by reduced bone mass, disruption of bone microarchitecture without alteration in the composition of bone. Osteoporotic bones are more at risk of fracture.
  Note=A chromosomal aberration involving COL1A1 is found in dermatofibrosarcoma protuberans. Translocation t(17;22)(q22;q13) with PDGF.
• Sequence similaritiesBelongs to the fibrillar collagen family.
  Contains 1 fibrillar collagen NC1 domain.
  Contains 1 VWFC domain.
• Post-translational
  modificationsProline residues at the third position of the tripeptide repeating unit (G-X-Y) are hydroxylated in some or all of the chains. Proline residues at the second position of the tripeptide repeating unit (G-X-Y) are hydroxylated in some of the chains.
  O-linked glycan consists of a Glc-Gal disaccharide bound to the oxygen atom of a post-translationally added hydroxyl group.
• Cellular localizationSecreted > extracellular space > extracellular matrix.

Target information above from: UniProt accession P02452 The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010) .

Information by UniProt
• Database links
  • Entrez Gene: 1278 Human
  • Entrez Gene: 1277 Human
  • Entrez Gene: 12843 Mouse
  • Entrez Gene: 12842 Mouse
  • Omim: 120150 Human
  • Omim: 120160 Human
  • SwissProt: P08123 Human
  • SwissProt: P02452 Human

  • SwissProt: Q01149 Mouse
  • SwissProt: P11087 Mouse
  • Unigene: 489142 Human
  • Unigene: 172928 Human
  • Unigene: 681002 Human
  • Unigene: 277735 Mouse
  • Unigene: 458212 Mouse

  see all

• Alternative names
  Alpha 1 type I collagen antibodyAlpha 2 type I collagen antibodyAlpha-1 type I collagen antibody

  CO1A1_HUMAN antibodyCOL1A1 antibodyCOL1A2 antibodyCollagen alpha-1(I) chain antibodyCollagen I alpha 1 polypeptide antibodyCollagen I alpha 2 polypeptide antibodyCollagen type I alpha 1 antibodyCollagen type I alpha 2 antibodyType I procollagen antibody

  see all

Anti-Collagen I antibody images

• 

  Western blot - Collagen I antibody (ab88147)
  Anti-Collagen I antibody (ab88147) at 1 µg/ml + immunogen (88 aa recombinant fragment with a proprietary tag of 26kDa) at 0.2 µg
  
  Secondary
  Goat anti-Mouse IgG at 1/5000 dilution
  
  Predicted band size : 139 kDa
  
• 

  Western blot - Collagen I antibody (ab88147)
  Anti-Collagen I antibody (ab88147) at 1 µg/ml + NIH 3T3 cell lysate at 25 µg
  
  Secondary
  Goat anti-Mouse IgG at 1/5000 dilution
  
  Predicted band size : 139 kDa
  
• 

  Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Collagen I antibody (ab88147)
  IHC image of Collagen I staining in human skin formalin fixed paraffin embedded tissue section, performed on a Leica Bond^TM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab88147, 5µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
  
  For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.