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A purified preparation of lathyritic type II collagen from embryonic chicken sternum.
Our Abpromise guarantee covers the use of ab54236 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IHC-P||Use at an assay dependent dilution. Perform enzymatic antigen retrieval before commencing with IHC staining protocol. Deparaffinization: Deparaffinize slides using xylene or xylene alternative and graded alcohols. Antigen Retrieval: Digest tissue sections with pepsin at 1mg/ml Tris-HCl, pH 2.0 for 15 min at RT or 10 min at 37°C. Primary Antibody Incubation: Incubate for 30 minutes at RT. Slide Washing: Slides must be washed in between steps. Rinse slides with PBS/0.05% Tween. Visualization: Detect the antibody as instructed by the instructions provided with the visualization system.|
ab54236 has not yet been referenced specifically in any publications.
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