For the best experience on the Abcam website please upgrade to a modern browser such as Google Chrome
Collagen Type II from human knee cartilage and bovine nasal cartilage.
Our Abpromise guarantee covers the use of ab34712 in the following tested applications.
|WB||1/5000 - 1/10000. Predicted molecular weight: 142 kDa. This product is not recommended for use under denaturing conditions in WB, IP, and ELISA. We would suggest testing it under native conditions.|
|ELISA||1/4000 - 1/8000.|
|IHC-P||1/50 - 1/400.|
|ICC/IF||Use at an assay dependent concentration.|
ab34712 (1:400, 45 min RT) showed moderate staining in FFPE human bronchiolar cartilage. Slides were steamed in 0.01 M sodium citrate buffer, pH 6.0 at 99-100°C - 20 minutes for antigen retrieval.
Immunofluorescence analysis of Human articular chondrocytes, staining Collagen II with ab34712.
Cells were fixed with paraformaldehyde and blocked for 1 hour with 1% BSA. Cells were incubated with primary antibody overnight at 4°C. An Alexa Fluor® 594-conjugated goat anti-rabbit polyclonal IgG (ab150080) was used as the secondary antibody.
ab34712 staining Collagen II in Chicken embryonic sternal chondrocyte P17 cells by ICC/IF (Immunocytochemistry/immunofluorescence). Cells were fixed with paraformaldehyde, permeabilzed with PBS + Tween 20 and blocked with 6% serum for 1 hour at 4°C. Samples were incubated with primary antibody (1/200) for 8 hours at 4°C. An Alexa Fluor®594-conjugated goat anti-rabbit IgG (ab150080) polyclonal (1/1000) was used as the secondary antibody.