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Collagen Type II from human knee cartilage and bovine nasal cartilage.
Our Abpromise guarantee covers the use of ab34712 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/5000 - 1/10000. Predicted molecular weight: 142 kDa. This product is not recommended for use under denaturing conditions in WB, IP, and ELISA. We would suggest testing it under native conditions.|
|ELISA||1/4000 - 1/8000.|
|IHC-P||1/50 - 1/400.|
|ICC/IF||Use at an assay dependent concentration.|
ab34712 (1:400, 45 min RT) showed moderate staining in FFPE human bronchiolar cartilage. Slides were steamed in 0.01 M sodium citrate buffer, pH 6.0 at 99-100°C - 20 minutes for antigen retrieval.
Immunofluorescence analysis of Human articular chondrocytes, staining Collagen II with ab34712.
Cells were fixed with paraformaldehyde and blocked for 1 hour with 1% BSA. Cells were incubated with primary antibody overnight at 4°C. An Alexa Fluor® 594-conjugated goat anti-rabbit polyclonal IgG (ab150080) was used as the secondary antibody.
ab34712 staining Collagen II in Chicken embryonic sternal chondrocyte P17 cells by ICC/IF (Immunocytochemistry/immunofluorescence). Cells were fixed with paraformaldehyde, permeabilzed with PBS + Tween 20 and blocked with 6% serum for 1 hour at 4°C. Samples were incubated with primary antibody (1/200) for 8 hours at 4°C. An Alexa Fluor®594-conjugated goat anti-rabbit IgG (ab150080) polyclonal (1/1000) was used as the secondary antibody.