Collagen III antibody [1E7-D7/Col3] (ab23445)

Product Name 

Collagen III antibody [1E7-D7/Col3]

Product type 

Primary antibodies

Description 

Mouse monoclonal [1E7-D7/Col3] to Collagen III

Immunogen 

Full length native protein (purified): Acid digested pepsin soluble human type III collagen

Reacts with 

Hu, Rat, Dog, Kg

Does not react with 
(species key)

Cow, Shp

Specificity 

ab23445 is highly specific for type III Collagen. It has been shown to have no cross ractivity with type I and V Collagens. There is no evidence for cross reactivity with other connective tissue proteins (laminin, fibronectin, elastin).

Epitope 

The epitope recognised by ab23445 is located approximately 100nm from the N terminal of the molecule.

Tested applications 
(see key)

ELISA, IHC-Fr, WB

Abreviews 

Customer reviews (see key)

Read about Abreviews

Application notes 
(see key)

Recommended dilutions
ELISA: 1/500.
IHC-Fr: Use at an assay dependent dilution.
Note: Usually ab23445 is used in unfixed tissue slides. If fixation of tissue is required, acetone or ethanol is recommended.
WB: Use at an assay dependent dilution. Predicted molecular weight: 134 kDa.

Not tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.

Positive control 
(see definition)

Frozen and unfixed human or kangaroo skin tissue slides.

Cellular localization 

Secreted; extracellular space, extracellular matrix

Research areas 

Stem Cells >> Mesenchymal Stem Cells >> Osteogenesis
Stem Cells >> Mesenchymal Stem Cells >> Chondrogenesis
Signal Transduction >> Cytoskeleton / ECM >> Extracellular Matrix >> ECM Proteins >> Collagen

Relevance 

Collagen III is a fibrillar collagen that is found in extensible connective tissues such as skin, lung, and the vascular system, frequently in association with type I Collagen. Mutations in the gene are associated with Ehlers Danlos syndrome type IV, and with aortic and arterial aneurysms. Although alternate transcripts have been detected for this gene, they are the result of mutations; these mutations alter splicing, often leading to the exclusion of multiple exons.

Database links 

Raised in 

Mouse

Clonality 

Monoclonal

Clone number 

1E7-D7/Col3

Myeloma 

NS1/1-Ag4-1

Isotype 

IgG1

Light chain type 

kappa

Purity 

Protein A purified

Storage buffer 

Preservative: 15mM Sodium Azide
Constituents: 500mM Sodium chloride, 10mM PBS, pH 7.4

Material safety datasheet (MSDS) for this product:
Sodium Azide MSDS

Form 

Liquid

Concentration 

1.000 mg/ml

Storage instructions 

Store at +4°C in the dark.

References for Collagen III antibody [1E7-D7/Col3] (ab23445)

  This clone has been used in: (two most recent references)

Werkmeister JA & Ramshaw JA Multiple antigenic determinants on type III collagen. Biochem J 274 ( Pt 3):895-8 (1991). PubMed: 1707272

Werkmeister JA et al. Structural stability of long-term implants of a collagen-based vascular prosthesis. J Long Term Eff Med Implants 1:107-19 (1991). PubMed: 10149053

See all 4 publication references for this product.


If you publish research using ab23445 please let us know so that we can cite the reference on this datasheet.

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