Anti-Collagen III antibody [1E7-D7/Col3] (ab23445)

Overview

• Product nameAnti-Collagen III antibody [1E7-D7/Col3]See all Collagen III primary antibodies ...
• Description
  Mouse monoclonal [1E7-D7/Col3] to Collagen III
• Specificityab23445 is highly specific for type III Collagen. It has been shown to have no cross ractivity with type I and V Collagens. There is no evidence for cross reactivity with other connective tissue proteins (laminin, fibronectin, elastin).
• Tested applicationsELISA, IHC-Fr, WB more details
• Species reactivity
  Reacts with: Rat, Dog, Human, Pig, Kangaroo
  

  Does not react with

  Sheep, Cow
• Immunogen

  Full length native protein (purified): Acid digested pepsin soluble human type III collagen

• EpitopeThe epitope recognised by ab23445 is located approximately 100nm from the N terminal of the molecule.
• Positive controlFrozen and unfixed human or kangaroo skin tissue slides.

Properties

• FormLiquid
• Storage instructionsStore at +4°C in the dark.
• Storage bufferPreservative: 15mM Sodium Azide
  Constituents: 500mM Sodium chloride, 10mM PBS, pH 7.4
• Concentration information loading...
• PurityProtein A purified
• Clonality Monoclonal
• Clone number1E7-D7/Col3
• MyelomaNS1/1-Ag4-1
• IsotypeIgG1
• Light chain typekappa
• Research Areas
  • Stem Cells
  • Mesenchymal Stem Cells
  • Chondrogenesis

  • Stem Cells
  • Mesenchymal Stem Cells
  • Osteogenesis

  • Signal Transduction
  • Cytoskeleton / ECM
  • Extracellular Matrix
  • ECM Proteins
  • Collagen

Applications

Target

• FunctionCollagen type III occurs in most soft connective tissues along with type I collagen.
• Involvement in diseaseDefects in COL3A1 are a cause of Ehlers-Danlos syndrome type 3 (EDS3) [MIM:130020]; also known as benign hypermobility syndrome. EDS is a connective tissue disorder characterized by hyperextensible skin, atrophic cutaneous scars due to tissue fragility and joint hyperlaxity. EDS3 is a form of Ehlers-Danlos syndrome characterized by marked joint hyperextensibility without skeletal deformity.
  Defects in COL3A1 are the cause of Ehlers-Danlos syndrome type 4 (EDS4) [MIM:130050]. EDS is a connective tissue disorder characterized by hyperextensible skin, atrophic cutaneous scars due to tissue fragility and joint hyperlaxity. EDS4 is the most severe form of the disease. It is characterized by the joint and dermal manifestations as in other forms of the syndrome, characteristic facial features (acrogeria) in most patients, and by proneness to spontaneous rupture of bowel and large arteries. The vascular complications may affect all anatomical areas.
  Defects in COL3A1 are a cause of susceptibility to aortic aneurysm abdominal (AAA) [MIM:100070]. AAA is a common multifactorial disorder characterized by permanent dilation of the abdominal aorta, usually due to degenerative changes in the aortic wall. Histologically, AAA is characterized by signs of chronic inflammation, destructive remodeling of the extracellular matrix, and depletion of vascular smooth muscle cells.
• Sequence similaritiesBelongs to the fibrillar collagen family.
  Contains 1 fibrillar collagen NC1 domain.
  Contains 1 VWFC domain.
• Post-translational
  modificationsProline residues at the third position of the tripeptide repeating unit (G-X-Y) are hydroxylated in some or all of the chains.
  O-linked glycan consists of a Glc-Gal disaccharide bound to the oxygen atom of a post-translationally added hydroxyl group.
• Cellular localizationSecreted > extracellular space > extracellular matrix.

Target information above from: UniProt accession P02461 The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010) .

Information by UniProt
• Database links
  • Entrez Gene: 1281 Human
  • Entrez Gene: 1281 Human
  • Entrez Gene: 1281 Human
  • Entrez Gene: 84032 Rat
  • Omim: 120180 Human
  • SwissProt: P02461 Human
  • SwissProt: P02461 Human
  • SwissProt: P02461 Human

  • SwissProt: P13941 Rat
  • Unigene: 443625 Human
  • Unigene: 3247 Rat

  see all

• Alternative names
  CO3A1_HUMAN antibodyCOL 3A1 antibodyCOL3A1 antibody

  Collagen alpha 1(III) chain antibodyCollagen alpha-1(III) chain antibodyCollagen III alpha 1 chain precursor antibodyCollagen III alpha 1 polypeptide antibodyCollagen type III alpha 1 (Ehlers Danlos syndrome type IV autosomal dominant) antibodyCollagen type III alpha 1 antibodyCollagen type III alpha antibodyEDS4A antibodyEhlers Danlos syndrome type IV, autosomal dominant antibodyFetal collagen antibodyType III collagen antibody

  see all

Anti-Collagen III antibody [1E7-D7/Col3] images

• 

  Immunohistochemistry (Frozen sections) - Anti-Collagen III antibody [1E7-D7/Col3] (ab23445)This image is courtesy of an anonymous Abreview
  Immunohistochemical analysis of frozen pig skin tissue, staining Collagen III with ab23445.
  
  Tissue was fixed with acetone and blocked with 10% serum for 10 minutes 20°C. Samples were incubated with primary antibody (1/500 in TBS, 0.02% Triton x-100, 2% BSA) for 16 hours at 4°C. An undiluted biotin-conjugated goat anti-mouse polyclonal IgG was used as the secondary antibody.

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