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Products:Signal Transduction >> Cytoskeleton / ECM >> Extracellular Matrix >> ECM Proteins >> Collagen
Overview
Product name
Anti-Collagen III antibody [1E7-D7/Col3]
See all Collagen III products (13) ...
Description
Mouse monoclonal [1E7-D7/Col3] to Collagen III
Specificity
ab23445 is highly specific for type III Collagen. It has been shown to have no cross ractivity with type I and V Collagens. There is no evidence for cross reactivity with other connective tissue proteins (laminin, fibronectin, elastin).
Tested applications
ELISA, IHC-Fr, WBmore details
Cross reactivity
Reacts with
Rat, Dog, Human, Kangaroo
Does not react with
Sheep, Cow
Immunogen
Full length native protein (purified): Acid digested pepsin soluble human type III collagen
Epitope
The epitope recognised by ab23445 is located approximately 100nm from the N terminal of the molecule.
Positive control
Frozen and unfixed human or kangaroo skin tissue slides.
Properties
Form
Liquid
Storage instructions
Store at +4°C in the dark.
Storage buffer
Preservative: 15mM Sodium Azide
Constituents: 500mM Sodium chloride, 10mM PBS, pH 7.4
Concentration
Concentration information loading...
Purity
Protein A purified
Clonality
Monoclonal
Clone number
1E7-D7/Col3
Myeloma
NS1/1-Ag4-1
Isotype
IgG1
Light chain type
kappa
Research areas
Stem Cells >> Mesenchymal Stem Cells >> Osteogenesis
Stem Cells >> Mesenchymal Stem Cells >> Chondrogenesis
Signal Transduction >> Cytoskeleton / ECM >> Extracellular Matrix >> ECM Proteins >> Collagen
Applications
Show applications key
Our Abpromise guarantee covers the use of ab23445 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
ELISA
ELISA: 1/500ELISA: 1/500
IHC-Fr: Use at an assay dependent dilution. (Note: Usually ab23445 is used in unfixed tissue slides. If fixation of tissue is required, acetone or ethanol is recommended.)
WB: Use at an assay dependent dilution. Predicted molecular weight: 134 kDa.
Target
Relevance
Collagen III is a fibrillar collagen that is found in extensible connective tissues such as skin, lung, and the vascular system, frequently in association with type I Collagen. Mutations in the gene are associated with Ehlers Danlos syndrome type IV, and with aortic and arterial aneurysms. Although alternate transcripts have been detected for this gene, they are the result of mutations; these mutations alter splicing, often leading to the exclusion of multiple exons.
Cellular localization
Secreted; extracellular space, extracellular matrix
Alternative names
- COL 3A1 antibody
- COL3A1 antibody
- Collagen alpha 1(III) chain antibody
see all
Database links
- Entrez Gene: 1281 Human
- Entrez Gene: 84032 Rat
- Omim: 120180 Human
- SwissProt: P02461 Human
- SwissProt: P13941 Rat
- Unigene: 443625 Human
- Unigene: 3247 Rat
References for Anti-Collagen III antibody [1E7-D7/Col3] (ab23445)
This product has been referenced in:
- Werkmeister JA & Ramshaw JA Multiple antigenic determinants on type III collagen. Biochem J 274 ( Pt 3):895-8 (1991).Read more (PubMed: 1707272) »
- Werkmeister JAet al. Structural stability of long-term implants of a collagen-based vascular prosthesis. J Long Term Eff Med Implants 1:107-19 (1991).Read more (PubMed: 10149053) »
See all 4 publications for this product
Publishing research using ab23445? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"